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重组

重组Anti-GDF9抗体[53/1] - BSA and Azide free (ab273472)

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Western blot - Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)
  • Flow Cytometry (Intracellular) - Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)
  • Western blot - Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)
  • Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [53/1] to GDF9 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-GDF9抗体[53/1] - BSA and Azide free
    参阅全部 GDF9 一抗

  • 描述

    小鼠单克隆抗体[53/1] to GDF9 - BSA and Azide free

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details
    不适用于: ICC/IF or IHC-P

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Human ovary, ovary cancer, testis and epididymis tissue lysate. Human recombinant GDFP-9 protein. Flow Cyt (intra): NIH:OVCAR-3 cells.

  • 常规说明

    ab273472 is the carrier-free version of ab273455.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273472于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 57, 70 kDa (predicted molecular weight: 52 kDa).
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 57, 70 kDa (predicted molecular weight: 52 kDa).
应用说明
Is unsuitable for ICC/IF or IHC-P.

靶标

  • 相关性

    GDF9, growth/differentiation factor 9, is a secreted member of TGFB superfamily. GDF9 is expressed in the ovary (in the oocyte at the type 3a primary follicle stage) and involved in normal ovulation, fertilization and female reproduction.

  • 细胞定位

    Secreted

  • 数据库链接

    • 别名

      • GDF-9 antibody
      • Growth factor 9 antibody
      • Growth/differentiation factor 9 antibody

    图片

    • Western blot - Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)
      Western blot - Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)
      All lanes : Anti-GDF9 antibody [53/1] (ab273455) at 1/1000 dilution

      Lane 1 : Human ovary tissue lysate
      Lane 2 : Human ovary cancer tissue lysate
      Lane 3 : Human testis tissue lysate
      Lane 4 : Human epididymis tissue lysate
      Lane 5 : Human brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

      Predicted band size: 52 kDa
      Observed band size: 57,70 kDa why is the actual band size different from the predicted?


      Exposure time: 26 seconds


      This data was produced using the same clone but in a different formulation, ab273455.

      The doublet bands are the precursor form of GDF 9 and have been described in the previous literature. (PMID: 24438375, 32350111)
      Negative control: Brain (PMID: 22732078).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)
      Flow Cytometry (Intracellular) - Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)

      This data was produced using the same clone but in a different formulation, ab273455. 

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells labelling GDF 9 with ab273455 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor®488, ab150113) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Western blot - Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)
      Western blot - Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)
      Anti-GDF9 antibody [53/1] (ab273455) at 1/1000 dilution + Human recombinant GDF9 protein, 20ng

      Secondary
      Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

      Predicted band size: 52 kDa


      Exposure time: 1 second


      This data was produced using the same clone but in a different formulation, ab273455.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)
      Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab273472？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab273472 尚未被引用在任何文献中。

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