重组Anti-GDF9抗体[53/1] (ab273455)
![Western blot - Anti-GDF9 antibody [53/1] (ab273455)](https://www.abcam.cn/ps/products/273/ab273455/Images/ab273455-445367-anti-gdf-9-antibody-531-western-blot-human-ovary-tissue-lysate-20-ug.jpg "Western blot - Anti-GDF9 antibody [53/1] (ab273455)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [53/1] to GDF9
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GDF9抗体[53/1]
参阅全部 GDF9 一抗 -
描述
小鼠单克隆抗体[53/1] to GDF9 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human ovary, ovary cancer, testis and epididymis tissue lysate. Human recombinant GDFP-9 protein. Flow Cyt (intra): NIH:OVCAR-3 cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59.94% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
53/1 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273455于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/1000.
|
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| WB |
1/1000. Detects a band of approximately 57, 70 kDa (predicted molecular weight: 52 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/1000. |
|
WB
1/1000. Detects a band of approximately 57, 70 kDa (predicted molecular weight: 52 kDa). |
靶标
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相关性
GDF9, growth/differentiation factor 9, is a secreted member of TGFB superfamily. GDF9 is expressed in the ovary (in the oocyte at the type 3a primary follicle stage) and involved in normal ovulation, fertilization and female reproduction. -
细胞定位
Secreted -
数据库链接
- Entrez Gene: 2661 Human
- SwissProt: O60383 Human
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别名
- GDF-9 antibody
- Growth factor 9 antibody
- Growth/differentiation factor 9 antibody
图片
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Western blot - Anti-GDF9 antibody [53/1] (ab273455)All lanes : Anti-GDF9 antibody [53/1] (ab273455) at 1/1000 dilution
Lane 1 : Human ovary tissue lysate
Lane 2 : Human ovary cancer tissue lysate
Lane 3 : Human testis tissue lysate
Lane 4 : Human epididymis tissue lysate
Lane 5 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 52 kDa
Observed band size: 57,70 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsThe doublet bands are the precursor form of GDF 9 and have been described in the previous literature. (PMID: 24438375, 32350111)
Negative control: Brain (PMID: 22732078).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Flow Cytometry (Intracellular) - Anti-GDF9 antibody [53/1] (ab273455)](https://www.abcam.cn/ps/products/273/ab273455/Images/ab273455-445365-AntiGDF9-antibody-53-1-flow-cytometry-nih-ovcar3-human.jpg)
Flow Cytometry (Intracellular) - Anti-GDF9 antibody [53/1] (ab273455)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells labelling GDF 9 with ab273455 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-GDF9 antibody [53/1] (ab273455)](https://www.abcam.cn/ps/products/273/ab273455/Images/ab273455-445366-anti-gdf-9-antibody-531-western-blot-human-recombinant-gdfp9-protein.jpg)
Western blot - Anti-GDF9 antibody [53/1] (ab273455)Anti-GDF9 antibody [53/1] (ab273455) at 1/1000 dilution + Human recombinant GDF9 protein, 20ng
Secondary
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 52 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-GDF9 antibody [53/1] (ab273455)](https://www.abcam.cn/ps/products/273/ab273455/Images/ab273455-456256-benefitsofrecombinantantibodies.jpg)
Anti-GDF9 antibody [53/1] (ab273455)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab273455 尚未被引用在任何文献中。