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重组RabMAb

重组Anti-GDF15抗体[EPR19939] - Low endotoxin，Azide free (ab223539)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody [EPR19939] - Low endotoxin, Azide free (ab223539)
  • Immunoprecipitation - Anti-GDF15 antibody [EPR19939] - Low endotoxin, Azide free (ab223539)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody [EPR19939] - Low endotoxin, Azide free (ab223539)
  • ELISA - Anti-GDF15 antibody [EPR19939] - Low endotoxin, Azide free (ab223539)
  • Anti-GDF15 antibody [EPR19939] - Low endotoxin, Azide free (ab223539)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19939] to GDF15 - Low endotoxin, Azide free
  • Suitable for: ELISA, IP, WB, IHC-P
  • Reacts with: Human

概述

  • 产品名称

    Anti-GDF15抗体[EPR19939] - Low endotoxin，Azide free
    参阅全部 GDF15 一抗

  • 描述

    兔单克隆抗体[EPR19939] to GDF15 - Low endotoxin，Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ELISA, IP, WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HepG2 and LNCaP whole cell lysates; human prostate cancer and placenta lysates; untreated HT1080 and treated with 200ng/ml Phorbol-12-myristate-13-acetate (TPA) for 24 hours whole cell lysates. IHC-P: Human placenta and prostate hyperplasia tissues. IP: HepG2 whole cell lysate.

  • 常规说明

    ab223539 is the carrier-free version of ab206414.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab223539于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 35, 12.5 kDa (predicted molecular weight: 34 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC tests show positive staining only on human placenta and prostate hyperplasia tissues, other tissues tested were negative.
说明
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 35, 12.5 kDa (predicted molecular weight: 34 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC tests show positive staining only on human placenta and prostate hyperplasia tissues, other tissues tested were negative.

靶标

  • 组织特异性

    Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.

  • 序列相似性

    Belongs to the TGF-beta family.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • GDF 15 antibody
      • GDF-15 antibody
      • Gdf15 antibody
      • GDF15_HUMAN antibody
      • Growth differentiation factor 15 antibody
      • Growth/differentiation factor 15 antibody
      • Macrophage inhibitory cytokine 1 antibody
      • MIC 1 antibody
      • Mic-1 antibody
      • MIC1 antibody
      • NAG 1 antibody
      • NAG-1 antibody
      • NAG1 antibody
      • NRG 1 antibody
      • NRG-1 antibody
      • NRG1 antibody
      • NSAID (nonsteroidal anti inflammatory drug) activated protein 1 antibody
      • NSAID antibody
      • NSAID regulated protein 1 antibody
      • NSAID-activated gene 1 protein antibody
      • NSAID-regulated gene 1 protein antibody
      • PDF antibody
      • PLAB antibody
      • Placental bone morphogenetic protein antibody
      • Placental bone morphogenic protein antibody
      • Placental TGF beta antibody
      • Placental TGF-beta antibody
      • Prostate differentiation factor antibody
      • PTGF beta antibody
      • PTGFB antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody [EPR19939] - Low endotoxin, Azide free (ab223539)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody [EPR19939] - Low endotoxin, Azide free (ab223539)

      Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling GDF15 with ab206414 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

      Cytoplasmic staining on human placenta is observed (PMID: 9593718). Positive staining on human placenta and prostate hyperplasia, other tissues tested were negative.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206414).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-GDF15 antibody [EPR19939] - Low endotoxin, Azide free (ab223539)
      Immunoprecipitation - Anti-GDF15 antibody [EPR19939] - Low endotoxin, Azide free (ab223539)

      GDF15 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab206414 at 1/30 dilution.

      Western blot was performed from the immunoprecipitate using ab206414 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: HepG2 whole cell lysate 10µg (Input).

      Lane 2: ab206414 IP in HepG2 whole cell lysate.

      Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab206414 in HepG2 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206414).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody [EPR19939] - Low endotoxin, Azide free (ab223539)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody [EPR19939] - Low endotoxin, Azide free (ab223539)

      This IHC data was generated using the same anti-GFD15 antibody clone [EPR19939] in a different buffer formulation (cat# ab206414).

      Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue labeling GDF15 with ab206414 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

      Cytoplasmic staining on human prostate hyperplasia is observed (PMID: 9593718). Positive staining on human placenta and prostate hyperplasia, other tissues tested were negative.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • ELISA - Anti-GDF15 antibody [EPR19939] - Low endotoxin, Azide free (ab223539)
      ELISA - Anti-GDF15 antibody [EPR19939] - Low endotoxin, Azide free (ab223539)

      ELISA analysis of ab206414 binding functionally active mature GDF15 (ab50077) at 1 µg/mL.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206414).

    • Anti-GDF15 antibody [EPR19939] - Low endotoxin, Azide free (ab223539)
      Anti-GDF15 antibody [EPR19939] - Low endotoxin, Azide free (ab223539)

    文献 (1)

    发表研究结果有使用 ab223539？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab223539 被引用在 1 文献中.

    • Dong G  et al. SCAP Mediated GDF15-Induced Invasion and EMT of Esophageal Cancer. Front Oncol 10:564785 (2020). PubMed: 33123476

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