Anti-GCNT3抗体(ab77728)
Key features and details
- Goat polyclonal to GCNT3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GCNT3抗体
参阅全部 GCNT3 一抗 -
描述
山羊多克隆抗体to GCNT3 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey
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免疫原
Synthetic peptide:
C-NKFDPKVDDNALQ
, corresponding to internal sequence amino acids 410-422 of Human GCNT3 (NP_004742.1). -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab77728 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77728于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
| 说明 |
|---|
应用说明
Peptide ELISA: Antibody detection limit dilution 1/64000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 51 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 51 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development. -
组织特异性
Primarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced. -
通路
Protein modification; protein glycosylation. -
序列相似性
Belongs to the glycosyltransferase 14 family. -
翻译后修饰
N-glycosylated. -
细胞定位
Golgi apparatus membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 487578 Dog
- Entrez Gene: 100068109 Horse
- Entrez Gene: 9245 Human
- Entrez Gene: 72077 Mouse
- Entrez Gene: 286976 Rat
- Entrez Gene: 495681 Xenopus laevis
- Omim: 606836 Human
- SwissProt: O95395 Human
see all -
别名
- 2010013H22Rik antibody
- 2210021I22Rik antibody
- 2210401J11Rik antibody
see all
图片
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Western blot - Anti-GCNT3 antibody (ab77728)Anti-GCNT3 antibody (ab77728) at 0.1 µg/ml + Human Duodenum lysate (in RIPA buffer) at 35 µg
Predicted band size: 51 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab77728 被引用在 2 文献中.
- Zhang Y et al. LINC00511 enhances LUAD malignancy by upregulating GCNT3 via miR-195-5p. BMC Cancer 22:389 (2022). PubMed: 35399076
- Li RW et al. The vitamin D receptor and inducible nitric oxide synthase associated pathways in acquired resistance to Cooperia oncophora infection in cattle. Vet Res 42:48 (2011). WB ; Cow . PubMed: 21414188