Anti-GCN5p抗体(ab63810)
Key features and details
- Rabbit polyclonal to GCN5p
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
概述
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产品名称
Anti-GCN5p抗体 -
描述
兔多克隆抗体to GCN5p -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Saccharomyces cerevisiae -
免疫原
Recombinant fragment corresponding to the N-terminal domain (1-300aa) of Saccharomyces cerevisiae GCN5p.
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阳性对照
- Saccharomyces cerevisiae extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6
Preservative: 0.1% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63810于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
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| 说明 |
|---|
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WB
1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa). |
靶标
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相关性
GCN5p is a histone acetyltransferase that contains a bromo domain and an N-acetyltransferase domain. It is recruited by a number of DNA-binding transcriptional activators, such as GCN4p and HAP2/3/4. GCN5p is a component of a number of different histone acetylation (HAT) complexes, namely, SAGA, SALSA and ADA. The SAGA complex, which is around 1.8 MDa in size, is required for the recruitment of the basal transcriptional machinery to around 10% of RNA polymerase II gene promoters in yeast. The activity of GCN5 may be dependent on its interactions within these multisubunit complexes. GCN5p acetylates histone H2B on K11 and K16 and histone H3 on K9, K14, K18, K23, K27 and K36. It also contributes to acetylation of histone H4 (at K8 and K16) and histone H2A.Z. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 853167 Saccharomyces cerevisiae
- SwissProt: Q03330 Saccharomyces cerevisiae
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别名
- ADA4 antibody
- GCN 5p antibody
- GCN5 antibody
see all
图片
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Western blot - Anti-GCN5p antibody (ab63810)Anti-GCN5p antibody (ab63810) at 1/500 dilution + Lysate from S. cerevisiae(BY4741)
Secondary
HRP conjugated Goat anti-Rabbit IgG at 1/5000 dilution
Predicted band size: 51 kDa -
Western blot - Anti-GCN5p antibody (ab63810)All lanes : Anti-GCN5p antibody (ab63810) at 1/5000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Saccharomyces cerevisiae extract
Predicted band size: 51 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab63810 被引用在 1 文献中.
- Bian C et al. Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation. EMBO J 30:2829-42 (2011). WB ; Saccharomyces cerevisiae . PubMed: 21685874