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Anti-GCLC抗体(ab53179)

Reviews (2)Q&A (2)References (34)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLC antibody (ab53179)
  • Western blot - Anti-GCLC antibody (ab53179)

Key features and details

  • Rabbit polyclonal to GCLC
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-GCLC antibody [EP13475] (ab190685)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-GCLC抗体
    参阅全部 GCLC 一抗

  • 描述

    兔多克隆抗体to GCLC

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Rat GCLC aa 250-350 (internal sequence). The exact sequence is proprietary.
    Database link: P48506

  • 阳性对照

    • IHC-P: Human testis tissue. WB: Kidney lysate

  • 常规说明

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab53179于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB (1)
Use at an assay dependent concentration.
说明
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration.

靶标

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLC antibody (ab53179)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLC antibody (ab53179)

    Immunohistochemistry of Human testis stained with ab53179 at 1/100

  • Western blot - Anti-GCLC antibody (ab53179)
    Western blot - Anti-GCLC antibody (ab53179)
    Anti-GCLC antibody (ab53179) at 1/25 dilution + lysate prepared from kidney

文献 (34)

发表研究结果有使用 ab53179？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab53179 被引用在 34 文献中.

  • Lohakul J  et al. Mitochondria-Targeted Hydrogen Sulfide Delivery Molecules Protect Against UVA-Induced Photoaging in Human Dermal Fibroblasts, and in Mouse Skin In Vivo. Antioxid Redox Signal 36:1268-1288 (2022). PubMed: 34235951
  • Ma R  et al. Antitumor effects of Andrographis via ferroptosis-associated genes in gastric cancer. Oncol Lett 22:523 (2021). PubMed: 34025790
  • Wu DM  et al. Nrf2 overexpression rescues the RPE in mouse models of retinitis pigmentosa. JCI Insight 6:N/A (2021). PubMed: 33491671
  • Esparza-Moltó PB  et al. Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition. PLoS Biol 19:e3001252 (2021). PubMed: 33983919
  • Shimura T  et al. Enhanced anti-cancer activity of andrographis with oligomeric proanthocyanidins through activation of metabolic and ferroptosis pathways in colorectal cancer. Sci Rep 11:7548 (2021). PubMed: 33824419
View all Publications for this product

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-GCLC antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
20 µg
Specification
Liver
Treatment
Acetaminophen for 24 hrs
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 23 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-GCLC antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryo)
Specification
Embryo
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 11 2008

Question

Would we be able to have a replacement antibody? But instead of the ab53179, could we replace it with an ab41463 please? As I feel this would be more suited to our experiments. If you could help with this it would be highly appreciated.   Thank you,  

Read More
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab41463. Currently ab41463 is backordered. You should expect to receive this shipment around Dec. 6. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More

Question

LOT NUMBER GR40310-2 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Multiple bands -Strong bands around 45KDa and 95KDa, GCLC band at 73KDa is very weak SAMPLE Mouse Liver lysates PRIMARY ANTIBODY Abcam ab53179 - Multiple concentrations used from 1:5000 to 1:20000, 1hour-4hour incubations, up to 1 hour of washing. DETECTION METHOD Licor Odessey ANTIBODY STORAGE CONDITIONS -20 freezer SAMPLE PREPARATION laemmli (50%), diluted in dH20. Samples heated to 80degrees for 10mins. AMOUNT OF PROTEIN LOADED 20ug/10ul ELECTROPHORESIS/GEL CONDITIONS 10% gel TRANSFER AND BLOCKING CONDITIONS We have used both 10% Milk and 10% BSA, overnight and for 1hour SECONDARY ANTIBODY IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/20000 dilution HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 10 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Primary concentration, development method (ECLplus to Odessey), Incubation times, Wash times, Blocking buffer (BSA to Milk)

Read More
Answer

Thank you for submitting your completed complaint questionnaire. It does appear that this antibody is not detecting GCLC as expected. If you have purchased this antibody within the last 6 months or so I would be happy to offer you a free replacement, credit or refund. In your reply, please include the purchase order number or Abcam order reference number associated with the initial order of ab53179 so I can process your request. Thanks! I look forward to hearing from you.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com