Anti-GCLC抗体(ab41463)
Key features and details
- Rabbit polyclonal to GCLC
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GCLC抗体
参阅全部 GCLC 一抗 -
描述
兔多克隆抗体to GCLC -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow
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免疫原
Synthetic peptide corresponding to Human GCLC aa 50-150 conjugated to keyhole limpet haemocyanin.
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阳性对照
- This antibody gave a positive signal in the following Human Tissue Lysates: Liver, Kidney and Lung (Adenocarcinoma). This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41463于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). |
靶标
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通路
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. -
疾病相关
Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450]. -
序列相似性
Belongs to the glutamate--cysteine ligase type 3 family. - Information by UniProt
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数据库链接
- Entrez Gene: 2729 Human
- Entrez Gene: 14629 Mouse
- Entrez Gene: 25283 Rat
- Omim: 606857 Human
- SwissProt: P48506 Human
- SwissProt: P97494 Mouse
- SwissProt: P19468 Rat
- Unigene: 654465 Human
see all -
别名
- EC 6.3.2.2 antibody
- Gamma ECS antibody
- Gamma glutamylcysteine synthetase antibody
see all
图片
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Western blot - Anti-GCLC antibody (ab41463)All lanes : Anti-GCLC antibody (ab41463) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Human lung tissue lysate (adenocarcinoma) - insoluble fraction (female, 80 years) (ab42923)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 73 kDa
Observed band size: 73 kDa
Additional bands at: 58 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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SDS download
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Datasheet download
文献 (36)
ab41463 被引用在 36 文献中.
- Shimura T et al. Radiation affects glutathione redox reaction by reduced glutathione peroxidase activity in human fibroblasts. J Radiat Res 63:183-191 (2022). PubMed: 34977941
- Cores Á et al. Enantioselective Synthesis and Pharmacological Evaluation of Aza-CGP37157-Lipoic Acid Hybrids for the Treatment of Alzheimer's Disease. Antioxidants (Basel) 11:N/A (2022). PubMed: 35052616
- Duarte P et al. Novel Series of Dual NRF2 Inducers and Selective MAO-B Inhibitors for the Treatment of Parkinson's Disease. Antioxidants (Basel) 11:N/A (2022). PubMed: 35204129
- Kageyama S et al. p62/SQSTM1-droplet serves as a platform for autophagosome formation and anti-oxidative stress response. Nat Commun 12:16 (2021). PubMed: 33397898
- Sitthideatphaiboon P et al. STK11/LKB1 Mutations in NSCLC Are Associated with KEAP1/NRF2-Dependent Radiotherapy Resistance Targetable by Glutaminase Inhibition. Clin Cancer Res 27:1720-1733 (2021). PubMed: 33323404