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重组RabMAb

重组Anti-GCAT抗体[EPR13450] - N-terminal (ab181094)

Submit a review Submit a question References (1)
Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
  • Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
  • Immunocytochemistry/ Immunofluorescence - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
  • Immunoprecipitation - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
  • Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13450] to GCAT - N-terminal
  • Suitable for: WB, ICC/IF, IP, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-GCAT抗体[EPR13450] - N-terminal
    参阅全部 GCAT 一抗

  • 描述

    兔单克隆抗体[EPR13450] to GCAT - N-terminal

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IF, IP, IHC-Pmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • BxPC-3, HepG2 and U87-MG cell lysates. Human fetal liver tissue lysate. Human heart and pancreas tissue. HepG2 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab181094于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF
1/50 - 1/100.
IP
1/40.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF
1/50 - 1/100.
IP
1/40.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 组织特异性

    Strongly expressed in heart, brain, liver and pancreas. Also found in lung.

  • 通路

    Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 2/2.

  • 序列相似性

    Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

  • 细胞定位

    Mitochondrion.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 2 amino 3 ketobutyrate CoA ligase antibody
      • 2 amino 3 ketobutyrate coenzyme A ligase, mitochondrial antibody
      • 2-amino-3-ketobutyrate coenzyme A ligase antibody
      • AKB ligase antibody
      • Aminoacetone synthase antibody
      • GCAT antibody
      • Glycine acetyltransferase antibody
      • glycine C acetyltransferase antibody
      • glycine C acetyltransferase (2 amino 3 ketobutyrate coenzyme A ligase) antibody
      • KBL_HUMAN antibody
      • KLB antibody
      • mitochondrial antibody
      see all

    图片

    • Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
      Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
      All lanes : Anti-GCAT antibody [EPR13450] - N-terminal (ab181094) at 1/5000 dilution

      Lane 1 : BxPC-3 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : U87-MG cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa

    • Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
      Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
      Anti-GCAT antibody [EPR13450] - N-terminal (ab181094) at 1/5000 dilution + Human fetal liver tissue lysate

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

      Immunohistochemical analysis of Human heart tissue, staining GCAT with ab181094 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

      Immunohistochemical analysis of Human pancreas tissue, staining GCAT with ab181094 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
      Immunocytochemistry/ Immunofluorescence - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

      Immunofluorescence analysis of HepG2 cells, staining GCAT (green) with ab181094 at 1/100 dilution. An Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

    • Immunoprecipitation - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
      Immunoprecipitation - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

      Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate (lane 1) or negative control (lane 2), labeling GCAT immunoprecipitated using ab181094 at 1/40 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
      Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

    文献 (1)

    发表研究结果有使用 ab181094？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab181094 被引用在 1 文献中.

    • Abdollahi P  et al. Phosphatase of regenerating liver-3 regulates cancer cell metabolism in multiple myeloma. FASEB J 35:e21344 (2021). PubMed: 33566385

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