AB250794

重组Anti-GCAP2抗体[EPR14571] - BSA and Azide free

Anti-GCAP2 antibody [EPR14571] - BSA and Azide free

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal GCAP2 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples. Cited in 1 publication.

查看别名

GCAP2, GUCA1B, Guanylyl cyclase-activating protein 2, GCAP 2, Guanylate cyclase activator 1B

2 Images

Supplier Data

Western blot - Anti-GCAP2 antibody [EPR14571] - BSA and Azide free (AB250794)

This data was developed using ab184958, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GCAP2 antibody [EPR14571] (<a href='/products/primary-antibodies/gcap2-antibody-epr14571-ab184958'>ab184958</a>) at 1/1000 dilution

All lanes:

Mouse eyeball lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-GCAP2 antibody [EPR14571] - BSA and Azide free (AB250794)

This data was developed using ab184958, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GCAP2 antibody [EPR14571] (<a href='/products/primary-antibodies/gcap2-antibody-epr14571-ab184958'>ab184958</a>) at 1/2000 dilution

All lanes:

Rat eyeball lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-GCAP2 antibody [EPR14571]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14571

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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产品详情

ab250794 is the carrier-free version of ab184958.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Guanylyl cyclase-activating protein 2 (GCAP2) also known as GUCA1B is a calcium-binding protein with a molecular weight of approximately 24 kDa. GCAP2 plays an important role in the visual system's phototransduction process. It is primarily expressed in the retina especially in photoreceptor cells. GCAP2 is part of a family of neuronal calcium sensor proteins that adjust their activities based on calcium concentration changes. These adjustments help regulate the sensitivity of photoreceptors to light.

Biological function summary

This protein modulates the activity of retinal guanylyl cyclases. In photoreceptors it forms complexes with these enzymes to regulate the synthesis of cyclic GMP in response to light. The availability of cyclic GMP is essential for the recovery phase of phototransduction which allows visual cells to respond to light changes efficiently. GCAP2 switching between active and inactive states adjusts the production of cyclic GMP depending on calcium levels inside the cells.

Pathways

GCAP2 features in the phototransduction cascade an essential pathway for converting light into visual signals. This cascade involves several proteins including rhodopsin transducin and phosphodiesterase. GCAP2’s role focuses on regulating guanylyl cyclases allowing the restoration of cyclic GMP levels which is important for returning ion channels to their open state after photon absorption. It is tightly connected to GCAP1 another guanylyl cyclase-activating protein with similar functions but they operate under slightly different calcium concentrations.

GCAP2 mutations have been linked to certain forms of cone and cone-rod dystrophies retinal degenerative disorders that affect vision. Changes in GCAP2 function influence cyclic GMP levels disrupting normal photoreceptor activity and survival. Mutations can interact with the rhodopsin promoter influencing disease progression and severity. Understanding GCAP2’s role provides potential targets for treating these retinal disorders and improving photoreceptor health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Stimulates two retinal guanylyl cyclases (GCs) GUCY2D and GUCY2F when free calcium ions concentration is low, and inhibits GUCY2D and GUCY2F when free calcium ions concentration is elevated (By similarity). This Ca(2+)-sensitive regulation of GCs is a key event in recovery of the dark state of rod photoreceptors following light exposure (By similarity). May be involved in cone photoreceptor response and recovery of response in bright light (By similarity).

See full target information GUCA1B

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 19:1264 PubMed31888532

2019

TOPK promotes metastasis of esophageal squamous cell carcinoma by activating the Src/GSK3β/STAT3 signaling pathway via γ-catenin.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Jiang,Jing Zhang,Jimin Zhao,Zhenzhen Li,Hanyong Chen,Yan Qiao,Xinhuan Chen,Kangdong Liu,Ziming Dong

View all publications

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重组Anti-GCAP2抗体[EPR14571] - BSA and Azide free