AB225864

重组Anti-GC-C抗体[EPR22056-278]

Anti-GC-C antibody [EPR22056-278]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal GC-C antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications.

查看别名

Guc2c, Gucy2c, Guanylyl cyclase C, GC-C, Heat-stable enterotoxin receptor, Intestinal guanylate cyclase, STA receptor

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (AB225864)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (AB225864)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling GC-C with ab225864 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelium of rat colon (PMID : 27246004) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (AB225864)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling GC-C with ab225864 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelium of mouse colon (PMID : 27246004) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (AB225864)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GC-C with ab225864 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin.

Negative control : no staining on mouse spleen (PMID : 26986842) is observed.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-GC-C antibody [EPR22056-278] (AB225864)

Blocking/Dilution buffer : 5% NFDM/TBST.

GC-C migrates as two differentially glycosylated proteins with molecular masses of 130 and 145 kDa (PMID : 24217248).

All lanes:

Western blot - Anti-GC-C antibody [EPR22056-278] (ab225864) at 1/1000 dilution

All lanes:

Rat colon lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 123 kDa

Observed band size: 130 kDa,145 kDa

false

Exposure time: 37s

Supplier Data

Western blot - Anti-GC-C antibody [EPR22056-278] (AB225864)

Exposure time : Lane 1 : 3 minutes; Lane 2 : 26 seconds.

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

GC-C migrates as a glycosylated 145 kDa protein and a 90 kDa cleavage product (PMID : 9344852; PMID : 26986842).

Negative control : mouse spleen.

All lanes:

Western blot - Anti-GC-C antibody [EPR22056-278] (ab225864) at 1/1000 dilution

Lane 1:

Mouse spleen lysate at 10 µg

Lane 2:

Mouse colon lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 123 kDa

Observed band size: 145 kDa,90 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-GC-C antibody [EPR22056-278] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22056-278

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GC-C also known as guanylate cyclase C is an enzyme with mass around 120 kDa expressed mainly in the intestinal epithelium. This membrane-bound receptor plays an important role in the regulation of fluid and electrolyte balance in the intestine. GC-C gets activated by its ligands including bacterial heat-stable enterotoxins as well as endogenous peptides like guanylin and uroguanylin. Upon activation GC-C catalyzes the conversion of GTP to cGMP thereby initiating a signaling cascade that affects ion transport in intestinal cells.

Biological function summary

GC-C is part of a signaling mechanism involved in maintaining fluid balance and gut homeostasis. The enzyme participates in the cGMP-mediated signaling pathway essential for modulating chloride and bicarbonate ion secretion leading to water loss into the intestinal lumen. GC-C does not work in isolation but cooperates with other molecules to facilitate these functions. Specifically it associates with ion transporters and channels influencing the enteric nervous system.

Pathways

GC-C functions within the cGMP pathway which is critical for intestinal fluid regulation. GC-C also connects with the Wnt signaling pathway an important metabolic relay influencing cell proliferation and differentiation. By producing cGMP GC-C influences down-stream proteins such as protein kinase G (PKG) and cystic fibrosis transmembrane conductance regulator (CFTR). These proteins play pivotal roles in maintaining the physiological processes in the intestine.

GC-C relates to irritable bowel syndrome (IBS) and colorectal cancer. Overactivation of GC-C by bacterial toxins can lead to enterotoxigenic diarrhea common in IBS. Its role in colorectal cancer arises from dysregulated signaling pathways affecting cellular growth. Mutations and alterations in GC-C expression may contribute to cancer progression. Furthermore GC-C-related dysregulation can involve other proteins like E-cadherin which modulates cell adhesion in tumor pathways complicating disease dynamics.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Guanylyl cyclase that catalyzes synthesis of cyclic GMP (cGMP) from GTP.

See full target information Gucy2c

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:1624 PubMed40855239

2025

DNA methylation regulates TREM1 expression to modulate immune responses and drive progression in colorectal neuroendocrine neoplasm as a potential therapeutic target.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Guo,Guiwen Zheng,Jia Li,Shuzhan Yao,Qiang Jia,Jian Tan,Zhaowei Meng

Nan fang yi ke da xue xue bao = Journal of Southern Medical University 41:376-383 PubMed33849828

2021

[Changes of guanylate cyclase C in colon tissues of rats with intestinal injury associated with severe acute pancreatitis].

Applications

Unspecified application

Species

Unspecified reactive species

B Wang,Q Huang,S Li,J Wu,X Yuan,H Sun,L Tang

View all publications

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重组Anti-GC-C抗体[EPR22056-278]