AB119236

Anti-GBP1抗体[OTI1B2]

Name: GBP1抗体[OTI1B2] (ab119236)| Abcam中文官网
Brand: Abcam Limited
SKU: AB119236
Price: 4864 CNY
Availability: InStock

Anti-GBP1 antibody [OTI1B2]

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(4 Publications)

Mouse Monoclonal GBP1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Transfected cell lysate, Transfected cell line samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GBP1.

查看别名

Guanylate-binding protein 1, GTP-binding protein 1, Guanine nucleotide-binding protein 1, Interferon-induced guanylate-binding protein 1, GBP-1, HuGBP-1, hGBP1, GBP1

6 Images

Flow Cytometry (Intracellular) - Anti-GBP1 antibody [OTI1B2] (AB119236)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-GBP1 antibody [OTI1B2] (AB119236)

Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY GBP1 (red) or empty vector control plasmid (blue) labeling GBP1 with ab119236 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP1 antibody [OTI1B2] (AB119236)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP1 antibody [OTI1B2] (AB119236)

Paraffin-embedded human lung carcinoma tissue stained for GBP1 using ab119236 at 1/150 in immunohistochemical analysis.

Supplier Data

Western blot - Anti-GBP1 antibody [OTI1B2] (AB119236)

All lanes:

Western blot - Anti-GBP1 antibody [OTI1B2] (ab119236) at 1/200 dilution

Lane 1:

Human testis extract at 10 µg

Lane 2:

Human omentum extract at 10 µg

Lane 3:

Human uterus extract at 10 µg

Lane 4:

Human breast extract at 10 µg

Lane 5:

Human brain extract at 10 µg

Lane 6:

Human liver extract at 10 µg

Lane 7:

Human ovary extract at 10 µg

Lane 8:

Human thyroid gland extract at 10 µg

Lane 9:

Human colon extract at 10 µg

Lane 10:

Human spleen extract at 10 µg

Predicted band size: 68 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-GBP1 antibody [OTI1B2] (AB119236)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GBP1 antibody [OTI1B2] (AB119236)

COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY GBP1 cDNA, stained for GBP1 using ab119236 at 1/100 in ICC/IF.

Unknown

Western blot - Anti-GBP1 antibody [OTI1B2] (AB119236)

All lanes:

Western blot - Anti-GBP1 antibody [OTI1B2] (ab119236) at 1/2000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY GBP1 cDNA at 5 µg

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-GBP1 antibody [OTI1B2] (AB119236)

All lanes:

Western blot - Anti-GBP1 antibody [OTI1B2] (ab119236) at 1/200 dilution

All lanes:

Mouse spleen extract at 10 µg

Predicted band size: 68 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

OTI1B2

亚型

IgG1

不含载体蛋白

反应种属

Mouse, Human

应用

IHC-P, WB, ICC/IF, Flow Cyt (Intra)

applications

免疫原

Recombinant Full Length Protein corresponding to Human GBP1.

P32455

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Clone OTI1B2 (formerly 1B2).

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

纯化说明

Purified from cell culture supernatant

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The target known commonly as GBP1 or guanylate-binding protein 1 plays a mechanical role as a large GTPase with a molecular mass of approximately 67 kDa. This protein is expressed in various tissues most notably in interferon-gamma-induced human cells. GBP1 localizes mainly in the cytoplasm and associates with cellular membranes. Alternate designations for the GBP1 protein include GBP 1. Its role as a GTPase involves hydrolyzing GTP which is critical for its cellular functions.

Biological function summary

The GBP1 protein supports cellular defense mechanisms against pathogens such as viruses and bacteria. GBP1 participates in the hydrolysis of GTP to GMP an action that influences its anti-pathogenic effects. It is a member of the interferon-inducible large GTPase family and can form oligomeric complexes enhancing its antiviral and antimicrobial activity. It interacts with various proteins linked to innate immunity and contributes to modulating the immune response.

Pathways

GBP1 is involved in pathways linked to interferon signaling and innate immunity. The protein plays a significant role in the JAK-STAT signaling pathway where it exerts its antiviral effects. GBP1 interacts closely with other proteins such as STAT1 and is responsive to interferon signals influencing gene expression and immune responses. Its activity in these pathways is critical for mounting an effective defense against infections.

GBP1 has been associated with inflammatory diseases and cancer. The protein's expression levels often increase in response to inflammatory signals implicating it in the pathogenesis of diseases like Crohn's disease. Moreover GBP1 has a connection with cancer-related mechanisms where it might affect tumor cell proliferation. In these contexts proteins such as STAT1 and other interferon-responsive proteins relate closely to the function and regulatory mechanisms of GBP1.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens (PubMed : 16511497, PubMed : 22106366, PubMed : 29144452, PubMed : 31268602, PubMed : 32510692, PubMed : 32581219, PubMed : 37797010, PubMed : 7512561). Hydrolyzes GTP to GMP in two consecutive cleavage reactions : GTP is first hydrolyzed to GDP and then to GMP in a processive manner (PubMed : 16511497, PubMed : 32510692, PubMed : 7512561). Following infection, recruited to the pathogen-containing vacuoles or vacuole-escaped bacteria and promotes both inflammasome assembly and autophagy (PubMed : 29144452, PubMed : 31268602). Acts as a positive regulator of inflammasome assembly by facilitating the detection of inflammasome ligands from pathogens (PubMed : 31268602, PubMed : 32510692, PubMed : 32581219). Involved in the lysis of pathogen-containing vacuoles, releasing pathogens into the cytosol (By similarity). Following pathogen release in the cytosol, forms a protein coat in a GTPase-dependent manner that encapsulates pathogens and promotes the detection of ligands by pattern recognition receptors (PubMed : 32510692, PubMed : 32581219). Plays a key role in inflammasome assembly in response to infection by Gram-negative bacteria : following pathogen release in the cytosol, forms a protein coat that encapsulates Gram-negative bacteria and directly binds to lipopolysaccharide (LPS), disrupting the O-antigen barrier and unmasking lipid A that is that detected by the non-canonical inflammasome effector CASP4/CASP11 (PubMed : 32510692, PubMed : 32581219). Also promotes recruitment of proteins that mediate bacterial cytolysis, leading to release double-stranded DNA (dsDNA) that activates the AIM2 inflammasome (PubMed : 31268602). Involved in autophagy by regulating bacteriolytic peptide generation via its interaction with ubiquitin-binding protein SQSTM1, which delivers monoubiquitinated proteins to autolysosomes for the generation of bacteriolytic peptides (By similarity). Confers protection to several pathogens, including the bacterial pathogens L.monocytogenes and M.bovis BCG as well as the protozoan pathogen T.gondii (PubMed : 31268602). Exhibits antiviral activity against influenza virus (PubMed : 22106366).

See full target information GBP1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40254393

2025

Reversible downregulation of HLA class I in adenoid cystic carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Annie Li,Bianca L Gonda,Elizabeth M Codd,Adam von Paternos,Dawn R Mitchell,Markus D Herrmann,Prinjali Kalyan,Samantha E Flynn,Thuc Q Dzu,Chengzhuo Gao,Edwin Zhang,Julia J Mendel,Julia C Thierauf,Peter M Sadow,Thomas Denize,Diane Yang,Florian J Fintelmann,Jo Anne Fordham,Ross D Merkin,Atul K Bhan,Yu-Chung Huang,Jeffrey Raizer,William C Faquin,Daniel L Faden,Xin Gao,Jong Chul Park,Lori J Wirth,Stefan T Kaluziak,A John Iafrate

Science immunology 8:eabo4767 PubMed37478192

2023

NLRP11 is a pattern recognition receptor for bacterial lipopolysaccharide in the cytosol of human macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Maricarmen Rojas-Lopez,María Luisa Gil-Marqués,Vritti Kharbanda,Amanda S Zajac,Kelly A Miller,Thomas E Wood,Austin C Hachey,Keith T Egger,Marcia B Goldberg

International immunopharmacology 114:109478 PubMed36462334

2022

IFIH1/IRF1/STAT1 promotes sepsis associated inflammatory lung injury via activating macrophage M1 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Ailing Wang,Xueli Kang,Jing Wang,Shi Zhang

PloS one 10:e0140036 PubMed26474164

2015

In-Depth, Label-Free Analysis of the Erythrocyte Cytoplasmic Proteome in Diamond Blackfan Anemia Identifies a Unique Inflammatory Signature.

Applications