重组Anti-GATA3 (phospho S308)抗体[EPR18118] (ab186371)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18118] to GATA3 (phospho S308)
- Suitable for: WB, IHC-P, Dot blot, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-GATA3 (phospho S308)抗体[EPR18118]
参阅全部 GATA3 一抗 -
描述
兔单克隆抗体[EPR18118] to GATA3 (phospho S308) -
宿主
Rabbit -
特异性
The immunogen sequence for this antibody is identical to the sequence around S340 of GATA2 (UniProt P23769); however the phosphorylation of GATA2 on S340 has not been reported.
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经测试应用
适用于: WB, IHC-P, Dot blot, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat whole cell lysate and EL4 cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours. IHC-P: Human placenta tissue and Human transitional cell carcinoma tissues; Mouse and rat stomach tissue. IP: Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR18118 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab186371于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Dot blot |
1/1000.
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IP |
1/70.
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说明 |
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WB
1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Dot blot
1/1000. |
IP
1/70. |
靶标
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功能
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. -
组织特异性
T-cells and endothelial cells. -
疾病相关
Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome. -
序列相似性
Contains 2 GATA-type zinc fingers. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2625 Human
- Entrez Gene: 14462 Mouse
- Entrez Gene: 85471 Rat
- Omim: 131320 Human
- SwissProt: P23771 Human
- SwissProt: P23772 Mouse
- Unigene: 524134 Human
- Unigene: 313866 Mouse
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形式
There are 2 isoforms produced by alternative splicing. -
别名
- GATA 3 antibody
- GATA binding factor 3 antibody
- GATA binding protein 3 antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling GATA3 (phospho S308) with ab186371 at 1/50 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.Nuclear staining on rat stomach without Lambda Protein Phosphatase treatment (image A). No signal was detected when tissues were treated with Lambda Protein Phosphatase treatment (image B).
The section was incubated with ab186371 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling GATA3 (phospho S308) with ab186371 at 1/50 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.Nuclear staining on mouse stomach without Lambda Protein Phosphatase treatment (image A). No signal was detected when tissues were treated with Lambda Protein Phosphatase treatment (image B).
The section was incubated with ab186371 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Dot blot analysis of GATA3 (phospho S308) using ab186371 at 1:1000 (1.121 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody (ab97051) at 1:100000 dilution.
Lane 1: Human GATA3 (phospho Ser316) peptide
Lane 2: Human GATA3 (phospho Thr315) peptide
Lane 3: Human GATA3 (phospho Ser308) peptideBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds
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All lanes : Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371) at 1/1000 dilution
Lane 1 : Untreated EL4 (mouse lymphoma T lymphocyte) whole cell lysate
Lane 2 : EL4 treated with 0.5 mM 8-Bromo-cAMP for 6 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 70 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371) at 1/10000 dilution
Lane 1 : Untreated Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
Lane 2 : Jurkat whole cell lysates treated with 1mM 8-Bromo-cAMP for 6 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 15 secondsBlocking/dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma tissue labeling GATA3 (phospho S308) with ab186371 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on tumor cells of Human transitional cell carcinoma of bladder is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling GATA3 (phospho S308) with ab186371 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on trophoblast cells of Human placenta is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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GATA3 (phospho S308) was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) cells treated with 1mM 8-Bromo-cAMP for 6 hours with ab186371 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab186371 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.
Lane 1: Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours, 10 µg (Input).
Lane 2: ab186371 IP in Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab186371 in Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 10 seconds.
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Dot blot analysis of GATA3 (phospho S308) peptide (Lane 1) and non-phospho peptide (Lane 2) labeled using ab186371 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST..
Exposure time: 3 minutes.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab186371 被引用在 1 文献中.
- Sereesongsaeng N et al. Cathepsin V suppresses GATA3 protein expression in luminal A breast cancer. Breast Cancer Res 22:139 (2020). PubMed: 33298139