Anti-Gastrin抗体(ab53085)
Key features and details
- Rabbit polyclonal to Gastrin
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Gastrin抗体
参阅全部 Gastrin 一抗 -
描述
兔多克隆抗体to Gastrin -
宿主
Rabbit -
特异性
ab53085 detects endogenous levels of total Gastrin protein. The immunogen for this antibody has high alignment to CKK. It is therefore predicted to detect CKK as well as Gastrin, though this has not been tested experimentally. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide derived from Human Gastrin
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53085于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
靶标
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功能
Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. -
序列相似性
Belongs to the gastrin/cholecystokinin family. -
翻译后修饰
Two different processing pathways probably exist in antral G-cells. In the dominant pathway progastrin is cleaved at three sites resulting in two major bioactive gastrins, gastrin-34 and gastrin-17. In the putative alternative pathway, progastrin may be processed only at the most C-terminal dibasic site resulting in the synthesis of gastrin-71.
Sulfation enhances proteolytic processing, and blocks peptide degradation. Levels of sulfation differ between proteolytically-cleaved gastrins. Thus, gastrin-6 is almost 73% sulfated, whereas the larger gastrins are less than 50% sulfated. Sulfation levels are also tissue-specific. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 14459 Mouse
- SwissProt: P48757 Mouse
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别名
- Component II antibody
- G14 antibody
- G17 antibody
see all
图片
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Western blot - Anti-Gastrin antibody (ab53085)All lanes : Anti-Gastrin antibody (ab53085) at 1/500 dilution
Lane 1 : NIH/3T3 cell extract
Lane 2 : NIH/3T3 cell extract with peptide
Predicted band size: 11 kDa
Observed band size: 11 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab53085 被引用在 3 文献中.
- Zhao Y et al. Gastrin/CCK-B Receptor Signaling Promotes Cell Invasion and Metastasis by Upregulating MMP-2 and VEGF Expression in Gastric Cancer. J Cancer 13:134-145 (2022). PubMed: 34976177
- Miao ZF et al. A Metformin-Responsive Metabolic Pathway Controls Distinct Steps in Gastric Progenitor Fate Decisions and Maturation. Cell Stem Cell 26:910-925.e6 (2020). PubMed: 32243780
- Lavoie EG et al. Ectonucleotidases in the digestive system: focus on NTPDase3 localization. Am J Physiol Gastrointest Liver Physiol 300:G608-20 (2011). IHC-Fr ; Mouse . PubMed: 21233276