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Anti-Gastrin抗体(ab16035)

Submit a review Q&A (2)References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrin antibody (ab16035)

    Key features and details

    • Rabbit polyclonal to Gastrin
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Gastrin antibody [SP318] (ab223501)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Gastrin抗体
      参阅全部 Gastrin 一抗

    • 描述

      兔多克隆抗体to Gastrin

    • 宿主

      Rabbit

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human
      预测可用于: Horse, Dog

    • 免疫原

      Synthetic peptide within Human Gastrin aa 50 to the C-terminus. The exact sequence is proprietary.
      Database link: P01350

    • 阳性对照

      • Pituitary or stomach

    • 常规说明

       

      This antibody may be used to study the differential tissue expression and intracellular and subcellular localization of gastrin in neuroendocrine cells of the gastrointestinal tract, and in the CNS. Antibodies to gastrin are also useful for the identificationand detection of gastrin in normal and neoplastic tissue.

      This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab16035于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/200.
    说明
    IHC-P
    1/200.

    靶标

    • 功能

      Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.

    • 序列相似性

      Belongs to the gastrin/cholecystokinin family.

    • 翻译后修饰

      Two different processing pathways probably exist in antral G-cells. In the dominant pathway progastrin is cleaved at three sites resulting in two major bioactive gastrins, gastrin-34 and gastrin-17. In the putative alternative pathway, progastrin may be processed only at the most C-terminal dibasic site resulting in the synthesis of gastrin-71.
      Sulfation enhances proteolytic processing, and blocks peptide degradation. Levels of sulfation differ between proteolytically-cleaved gastrins. Thus, gastrin-6 is almost 73% sulfated, whereas the larger gastrins are less than 50% sulfated. Sulfation levels are also tissue-specific.

    • 细胞定位

      Secreted.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Component II antibody
        • G14 antibody
        • G17 antibody
        • G34 antibody
        • G52 antibody
        • G6 antibody
        • GAS antibody
        • Gast antibody
        • GAST_HUMAN antibody
        • Gastrin antibody
        • Gastrin component I antibody
        • Gastrin component II antibody
        • Gastrin component III antibody
        • Gastrin precursor antibody
        • Gastrin-14 antibody
        • Gastrin-17 antibody
        • Gastrin-34 antibody
        • Gastrin-52 antibody
        • Gastrin-6 antibody
        • Gastrin-71 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrin antibody (ab16035)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrin antibody (ab16035)

        ab16035 staining human Gastrin at 1/200 dilution in stomach by Immunohistochemistry (FFPE-sections).

      文献 (2)

      发表研究结果有使用 ab16035？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab16035 被引用在 2 文献中.

      • Poradowski D & Chrószcz A Equine Stomach Development in the Foetal Period of Prenatal Life-An Immunohistochemical Study. Animals (Basel) 13:N/A (2022). PubMed: 36611768
      • Lennerz JK  et al. The Transcription Factor MIST1 Is a Novel Human Gastric Chief Cell Marker Whose Expression Is Lost in Metaplasia, Dysplasia, and Carcinoma. Am J Pathol 177:1514-33 (2010). IHC-P ; Human . PubMed: 20709804

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Question

      PLease let me know whether this product has cross reactivity to CCK or not.
      Thnks and regards.

      Read More
      Answer

      Thank you for contacting us.

      We do not have any experimental data regarding the cross-reactivity of ab16035 with CCK. I compared the immunogen sequence for ab16035 with that of the CCK sequence (P06307-Uniprot) and the percentage homology is 50%, thus it is unlikely that it will cross-react with CCK.

      Please do not hesitate to contact me with additional questions or concerns.

      Read More

      Question

       Thank you for your quick responce. Actually what we are concern
      is whether cholecystokinin (CCK) can give any faulty result with this product ab16035.
      As cholecystokinin (CCK) has the sequence homology with gastrin and in the data sheet
      it is also mentioned"Sequence similarities : Belongs to the gastrin/cholecystokinin family."
      And the customer requirement isantibody should be non reactive to CCK.So please reconfirm us
      whether we can offer him this product or not.

      Regards

      Read More
      Answer

      Thank you for contacting us.

      We have not experimentally tested the possibility of ab16035 cross reactivity with CCK. So I am sorry we cannot confirm it 100%. The recommendations we gave were based on the minimal sequence homology between CCK and Gastrin. There is no sequence homology between Gastrin and CCK in the N-terminal region, so it is more likely that antibodies raised to these regions will not be cross-reacting.

      The Gasrin sequence I was looking at in given at the following link; http://www.uniprot.org/uniprot/P01350 and the CCK link is http://www.uniprot.org/uniprot/P06307

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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