AB249500

重组Anti-GART抗体[EPR11622] - BSA and Azide free

Anti-GART antibody [EPR11622] - BSA and Azide free

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal GART antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

查看别名

PGFT, PRGS, GART, Trifunctional purine biosynthetic protein adenosine-3

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] - BSA and Azide free (AB249500)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] - BSA and Azide free (AB249500)

This data was developed using ab169550, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labeling GART with ab169550 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] - BSA and Azide free (AB249500)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] - BSA and Azide free (AB249500)

This data was developed using ab169550, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling GART with ab169550 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] - BSA and Azide free (AB249500)

This data was developed using ab169550, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling GART with ab169550 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-GART antibody [EPR11622] - BSA and Azide free (AB249500)

This data was developed using ab169550, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GART antibody [EPR11622] (<a href='/products/primary-antibodies/gart-antibody-epr11622-ab169550'>ab169550</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 107 kDa

false

Unknown

Western blot - Anti-GART antibody [EPR11622] - BSA and Azide free (AB249500)

This data was developed using ab169550, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GART antibody [EPR11622] (<a href='/products/primary-antibodies/gart-antibody-epr11622-ab169550'>ab169550</a>) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from HeLa cell lysate

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

Predicted band size: 107 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-GART antibody [EPR11622]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11622

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

ICC/IF, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

产品详情

ab249500 is the carrier-free version of ab169550.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GART also known as phosphoribosylglycinamide formyltransferase is a multifunctional enzyme with a mass of approximately 100 kDa. It plays a significant mechanical role in purine biosynthesis. GART is primarily expressed in rapidly dividing cells and tissues with high metabolic activity. It is one of three enzymes encoded by the GART gene contributing to the trifunctional protein complex that includes glycinamide ribonucleotide synthetase (GARS) and aminoimidazole ribonucleotide synthetase (AIRS). These enzyme functions are critical for the mechanistic steps in purine metabolism increasing their importance in synthesizing nucleotides for DNA and RNA production.

Biological function summary

GART is involved in synthesizing purine nucleotides through its role in converting phosphoribosylamine to phosphoribosylglycinamide. It does this by catalyzing the transfer of a formyl group highlighting its operation as part of a trifunctional protein. The complex formation with enzymes GARS and AIRS enables efficient handling of intermediates therefore fostering a streamlined biosynthetic process. This contributes directly to the cellular nucleotide pool which is fundamental for cell proliferation signaling and energy transfer.

Pathways

GART fits into the purine biosynthetic pathway which is essential for nucleotide synthesis. This function links it closely with proteins such as IMP dehydrogenase which further processes inosine monophosphate to produce different purine nucleotides. It is also involved in folate metabolism as it requires folate derivatives for its formyl transferase activity. The close association with these pathways illustrates GART's integral role in both cellular growth and maintenance of genetic material.

GART has implications in cancer and neurological disorders due to its role in nucleotide synthesis and cellular proliferation. Elevated expression levels of GART are observed in tumors indicating its potential involvement in oncogenesis. In addition abnormalities in purine metabolism or folate cycling can contribute to disorders like Down syndrome. Within this context proteins interacting with GART such as GARS and AIRS could potentially serve as associated targets for therapeutic intervention emphasizing the enzyme's relevance in disease pathology.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Trifunctional enzyme that catalyzes three distinct reactions as part of the 'de novo' inosine monophosphate biosynthetic pathway.

See full target information GART

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-GART抗体[EPR11622] - BSA and Azide free