Anti-GAPR-1抗体(ab122059)
Key features and details
- Rabbit polyclonal to GAPR-1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GAPR-1抗体 -
描述
兔多克隆抗体to GAPR-1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human GAPR-1 aa 2-73.
Sequence:GKSASKQFHNEVLKAHNEYRQKHGVPPLKLCKNLNREAQQYSEALASTRI LKHSPESSRGQCGENLAWASYD
Database link: Q9H4G4 -
阳性对照
- Transfected HEK293T cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122059于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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组织特异性
Highest expression in lung and peripheral leukocytes, and minor expression in liver and kidney. -
序列相似性
Belongs to the CRISP family.
Contains 1 SCP domain. -
细胞定位
Golgi apparatus membrane. Binds lipid-enriched microdomains of Golgi membranes not only by ionic interactions but also through the myristate. - Information by UniProt
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数据库链接
- Entrez Gene: 152007 Human
- Omim: 607141 Human
- SwissProt: Q9H4G4 Human
- Unigene: 493819 Human
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别名
- 5730414A08Rik antibody
- C77180 antibody
- C9orf19 antibody
see all
图片
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Western blot - Anti-GAPR-1 antibody (ab122059)All lanes : Anti-GAPR-1 antibody (ab122059) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : GAPR-1-transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique. -
Western blot - Anti-GAPR-1 antibody (ab122059)This image is courtesy of an anonymous AbreviewAll lanes : Anti-GAPR-1 antibody (ab122059) at 1/1000 dilution
Lane 1 : Mouse cell lysate
Lanes 2-3 : Human cell lysate
Lysates/proteins at 20000000 cells per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab122059 被引用在 1 文献中.
- Zhao D et al. miR-30e targets GLIPR-2 to modulate diabetic nephropathy: in vitro and in vivo experiments. J Mol Endocrinol 59:181-190 (2017). PubMed: 28733476