Anti-GAPDH抗体- Loading Control (ab83956)
Key features and details
- Chicken polyclonal to GAPDH - Loading Control
- Suitable for: WB
- Reacts with: Human
- Isotype: IgY
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GAPDH抗体- Loading Control
参阅全部 GAPDH 一抗 -
描述
鸡多克隆抗体to GAPDH - Loading Control -
宿主
Chicken -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
A 16 amino acid synthetic peptide from near the carboxy-terminus of Human GAPDH (Swiss-Prot P04406).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab83956于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 36 kDa.
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| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 36 kDa. |
靶标
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功能
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
序列相似性
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
翻译后修饰
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
细胞定位
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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数据库链接
- Entrez Gene: 2597 Human
- Omim: 138400 Human
- SwissProt: P04406 Human
- Unigene: 544577 Human
- Unigene: 592355 Human
- Unigene: 598320 Human
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别名
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
图片
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Western blot - Anti-GAPDH antibody - Loading Control (ab83956)Anti-GAPDH antibody - Loading Control (ab83956) at 1 µg/ml + HeLa Cell Lysate at 15 µg
Predicted band size: 36 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab83956 被引用在 8 文献中.
- Hasin N et al. Rare variants implicate NMDA receptor signaling and cerebellar gene networks in risk for bipolar disorder. Mol Psychiatry 27:3842-3856 (2022). PubMed: 35546635
- Burakov A et al. Persistent growth of microtubules at low density. Mol Biol Cell 32:435-445 (2021). PubMed: 33439670
- Wright CB et al. Chronic Dicer1 deficiency promotes atrophic and neovascular outer retinal pathologies in mice. Proc Natl Acad Sci U S A 117:2579-2587 (2020). PubMed: 31964819
- Syeda T et al. PhIP exposure in rodents produces neuropathology potentially relevant to Alzheimer's disease. Toxicology 437:152436 (2020). PubMed: 32169473
- Bachmann SO et al. Behavioral training rescues motor deficits in Cyfip1 haploinsufficiency mouse model of autism spectrum disorders. Transl Psychiatry 9:29 (2019). PubMed: 30664619
- Arulsamy A et al. Evaluation of early chronic functional outcomes and their relationship to pre-frontal cortex and hippocampal pathology following moderate-severe traumatic brain injury. Behav Brain Res 348:127-138 (2018). WB . PubMed: 29653127
- Osório C et al. Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Mol Cell Neurosci 59:24-36 (2014). WB ; Mouse . PubMed: 24444792
- McCoy DD et al. Enhanced insulin clearance in mice lacking TRPM8 channels. Am J Physiol Endocrinol Metab 305:E78-88 (2013). PubMed: 23651844