Anti-GAPDH抗体- Loading Control (ab70700)
Key features and details
- Rabbit polyclonal to GAPDH - Loading Control
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-GAPDH抗体- Loading Control
参阅全部 GAPDH 一抗 -
描述
兔多克隆抗体to GAPDH - Loading Control -
宿主
Rabbit -
特异性
We have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Gerbil, Chinese hamster
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免疫原
Synthetic peptide within Mouse GAPDH aa 300-335 (C terminal). The exact sequence is proprietary.
Database link: P16858 -
阳性对照
- Whole cell lysate from mouse NIH3T3 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70700于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
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| 说明 |
|---|
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IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/2000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). |
靶标
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功能
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
序列相似性
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
翻译后修饰
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
细胞定位
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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数据库链接
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 24383 Rat
- Entrez Gene: 685186 Rat
- SwissProt: P16858 Mouse
- SwissProt: P04797 Rat
- Unigene: 304088 Mouse
- Unigene: 309092 Mouse
see all -
别名
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (ab70700)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse squamous cell carcinoma tissue labelling GAPDH with ab70700 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-GAPDH antibody - Loading Control (ab70700)All lanes : Anti-GAPDH antibody - Loading Control (ab70700) at 0.04 µg/ml
Lane 1 : Whole cell lysate from mouse NIH3T3 cells. at 50 µg
Lane 2 : Whole cell lysate from mouse NIH3T3 cells. at 15 µg
Lane 3 : Whole cell lysate from mouse NIH3T3 cells. at 5 µg
Predicted band size: 36 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab70700 被引用在 1 文献中.
- Liu L et al. Identification of microRNAs enriched in exosomes in human pericardial fluid of patients with atrial fibrillation based on bioinformatic analysis. J Thorac Dis 12:5617-5627 (2020). PubMed: 33209394