Anti-GAPDH抗体- Loading Control (ab37168)
Key features and details
- Rabbit polyclonal to GAPDH - Loading Control
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GAPDH抗体- Loading Control
参阅全部 GAPDH 一抗 -
描述
兔多克隆抗体to GAPDH - Loading Control -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human GAPDH aa 60-110 (N terminal). Synthetic peptide, corresponding to 16 N terminal amino acids of Human GAPDH
Database link: P04406 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
GAPDH Antibody is affinity chromatography purified via peptide column. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab37168于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
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| IHC-P |
Use a concentration of 10 µg/ml.
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| ICC/IF |
Use a concentration of 0.1 - 1 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
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IHC-P
Use a concentration of 10 µg/ml. |
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ICC/IF
Use a concentration of 0.1 - 1 µg/ml. |
靶标
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功能
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
序列相似性
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
翻译后修饰
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
细胞定位
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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数据库链接
- Entrez Gene: 2597 Human
- Omim: 138400 Human
- SwissProt: P04406 Human
- Unigene: 544577 Human
- Unigene: 592355 Human
- Unigene: 598320 Human
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别名
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
图片
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Western blot - Anti-GAPDH antibody - Loading Control (ab37168)Lane 1 : Anti-GAPDH antibody - Loading Control (ab37168) at 0.125 µg/ml
Lane 2 : Anti-GAPDH antibody - Loading Control (ab37168) at 0.25 µg/ml
Lane 3 : Anti-GAPDH antibody - Loading Control (ab37168) at 0.5 µg/ml
All lanes : HeLa cell lysate with GAPDH peptide
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab37168)ICC/IF image of ab37168 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37168, 1mg/ml) overnight at +4øC. The secondary antibody (green) was anti-rabbit Alexa Fluor® 488 (ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
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Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab37168)ICC/IF image of ab37168 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37168, 1µg/ml) overnight at +4°C. The secondary antibody (green) was anti-Rabbit DyLight® 488 pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab37168)Immunofluorescence of GAPDH in HeLa cells using ab37168 at 10 ug/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (ab37168)ab37168 at 10µg/ml staining GAPDH in human liver tissue by IHC
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (472)
ab37168 被引用在 472 文献中.
- Zhu M et al. The P38MAPK/ATF2 signaling pathway is involved in PND in mice. Exp Brain Res 242:109-121 (2024). PubMed: 37973625
- You L et al. GLP-1 rescued gestational diabetes mellitus-induced suppression of fetal thalamus development. J Biochem Mol Toxicol 37:e23258 (2023). PubMed: 36424357
- Chai X et al. Inhibition of HIST1H2AB suppresses the proliferation of lung adenocarcinoma. J Gene Med 25:e3469 (2023). PubMed: 36511295
- Ren F et al. Proliferation, apoptosis and invasion of human lung cancer cells are associated with NFATc1. Exp Ther Med 25:49 (2023). PubMed: 36588811
- Li C et al. MicroRNA‑194‑5p attenuates hypoxia/reoxygenation‑induced apoptosis in H9C2 cardiomyocytes by inhibiting the over‑activation of RAC1 protein. Mol Med Rep 27:N/A (2023). PubMed: 36562344