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Anti-GAPDH抗体- Loading Control (ab22555)

Reviews (4)Q&A (1)References (115)
Western blot - Anti-GAPDH antibody - Loading Control (ab22555)
  • Western blot - Anti-GAPDH antibody - Loading Control (ab22555)

Key features and details

  • Rabbit polyclonal to GAPDH - Loading Control
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Dog, Human, African green monkey
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-GAPDH抗体- Loading Control
    参阅全部 GAPDH 一抗

  • 描述

    兔多克隆抗体to GAPDH - Loading Control

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Dog, Human, African green monkey
    预测可用于: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Turkey, Pig, Duck

  • 免疫原

    Synthetic peptide:

    DAPMFVMGVNHFKYA

    , corresponding to amino acids 126-140 of Human GAPDH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab22555于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (4)
1/1000 - 1/5000. Detects a band of approximately 36 kDa.
说明
WB
1/1000 - 1/5000. Detects a band of approximately 36 kDa.

靶标

图片

  • Western blot - Anti-GAPDH antibody - Loading Control (ab22555)
    Western blot - Anti-GAPDH antibody - Loading Control (ab22555)
    Lane 1 : Anti-GAPDH antibody - Loading Control (ab22555)
    Lanes 2-4 : Anti-GAPDH antibody - Loading Control (ab22555) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HUVEC cell lysate
    Lane 4 : PC12 cell lysate

    Lysates/proteins at 25 µg per lane.
  • Western blot - Anti-GAPDH antibody - Loading Control (ab22555)
    Western blot - Anti-GAPDH antibody - Loading Control (ab22555)
    All lanes : Anti-GAPDH antibody - Loading Control (ab22555) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : COS-7 cell lysate
    Lane 3 : MDCK cell lysate
    Lane 4 : PC-3 cell lysate
    Lane 5 : Mouse brain tissue lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ HRP conjugate at 1/4000 dilution

    Observed band size: 37 kDa why is the actual band size different from the predicted?

文献 (115)

发表研究结果有使用 ab22555？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab22555 被引用在 115 文献中.

  • Fu T  et al. LncRNA PVT1 induces apoptosis and inflammatory response of bronchial epithelial cells by regulating miR-30b-5p/BCL2L11 axis in COPD. Genes Environ 45:24 (2023). PubMed: 37817266
  • McHugh D  et al. COPI vesicle formation and N-myristoylation are targetable vulnerabilities of senescent cells. Nat Cell Biol 25:1804-1820 (2023). PubMed: 38012402
  • Pinheiro-de-Sousa I  et al. Integrated systems biology approach identifies gene targets for endothelial dysfunction. Mol Syst Biol 19:e11462 (2023). PubMed: 38031960
  • Su C  et al. HMGB1 Promotes Lymphangiogenesis through the Activation of RAGE on M2 Macrophages in Laryngeal Squamous Cell Carcinoma. Dis Markers 2022:4487435 (2022). PubMed: 35280439
  • Full Issue PDF. JACC Basic Transl Sci 7:I-CXVI (2022). PubMed: 35368970
View all Publications for this product

客户评价及客户问答

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-GAPDH antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (4-17%)
Loading amount
10 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 16 2018

Western blot abreview for Anti-GAPDH antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10%SDS gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 30 2010

Western blot abreview for Anti-GAPDH antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 15 2010

Western blot abreview for Anti-GAPDH antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Baboon Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 08 2010

Question

Customer kindly called to find replacement product for ab36840 in use on planaria.

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Answer

Thank you very much for your interest in anti-GAPDH antibodies.

I have reviewed the products in our catalog in effort to try an find for you a product which was most similar to ab36840. I believe that the following two products offer the best alternative.

AB75834 (https://www.abcam.com/gapdh-antibody-epr1977y-ab75834.html). This rabbit monoclonal was raised against a very similar region to ab36840. Therefore it may be likely that the same epitope site will be recogized by this product.

AB22555 (https://www.abcam.com/gapdh-antibody-ab22555.html). This rabbit polyclonal was raised against an area just before that which ab36840 was. While this product will not be recognizing the same epitopes it's polyclonal nature will mean that it will recognize more sites than a monoclonal antibody.

To our knowledge, these have not been tested in planaria. Therefore, I can offer a discount off a future purchase if you buy an GAPDH antibody now, test it in planaria and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test one of these in planaria. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

2. Purchase either by phone, fax, or online (www.abcam.com).

3. Test it in planaria.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the product turns out to be unsuitable for planaria, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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