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AB125247

Anti-GAPDH 抗体 [GA1R] - Loading Control

Anti-GAPDH antibody [GA1R] - Loading Control

5

(7 Reviews)

|

(132 Publications)

Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) is a mouse monoclonal antibody detecting GAPDH in Western Blot. Suitable for Chicken, Escherichia coli, Hamster, Human, Mouse, Rabbit, Rat, S.cerevisiae, Spodoptera frugiperda (SF9 cells).

- Over 130 publications

查看别名

GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

2 Images
Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (AB125247)
  • WB

Unknown

Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (AB125247)

All lanes:

Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution

Lane 1:

BL21 bacterial lysate at 5 µg

Lane 2:

Sf9 insect lysate at 5 µg

Lane 3:

Saccharomyces cerevisiae lysate at 5 µg

Predicted band size: 36 kDa

true

Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (AB125247)
  • WB

Unknown

Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (AB125247)

All lanes:

Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution

Lane 1:

Human tissue lysate at 5 µg

Lane 2:

Mouse tissue lysate at 5 µg

Lane 3:

Rat tissue lysate at 5 µg

Lane 4:

Rabbit tissue lysate at 5 µg

Lane 5:

Chicken tissue lysate at 5 µg

Lane 6:

Hamster tissue lysate at 5 µg

Predicted band size: 36 kDa

true

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

GA1R

亚型

IgG1

不含载体蛋白

No

反应种属

Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Escherichia coli, Fall armyworm

应用

WB

applications

免疫原

Recombinant Full Length Protein corresponding to Rabbit GAPDH.

P46406

特异性

ab125247 recognizes native and denatured forms of GAPDH.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>with ECL.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>with ECL.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>with ECL.</p>" }, "Chicken": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>with ECL.</p>" }, "Escherichia coli": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>with ECL.</p>" }, "Fall armyworm": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>with ECL.</p>" }, "Rabbit": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>with ECL.</p>" }, "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>with ECL.</p>" } } }
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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
纯化说明
Protein A affinity chromatography from TCS
存储溶液
pH: 7.2 Preservative: 0.05% Sodium azide Constituents: 99% PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed : 11724794, PubMed : 3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed : 11724794, PubMed : 3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed : 23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed : 23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed : 23332158, PubMed : 27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
See full target information GAPDH
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文献 (132)

Recent publications for all applications. Explore the full list and refine your search

Brain research 1822:148638 PubMed37858856

2023

Effects of acute Δ-tetrahydrocannabinol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Barkha J Yadav-Samudrala,Benjamin L Gorman,Hailey Dodson,Shreya Ramineni,E Diane Wallace,Michelle R Peace,Justin L Poklis,Wei Jiang,Sylvia Fitting

Pflugers Archiv : European journal of physiology 475:1225-1240 PubMed37566113

2023

The context-dependent role of the Na/Ca-exchanger (NCX) in pancreatic stellate cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Thorsten Loeck,Micol Rugi,Luca Matteo Todesca,Paulina Kalinowska,Benjamin Soret,Ilka Neumann,Sandra Schimmelpfennig,Karolina Najder,Zoltán Pethő,Valerio Farfariello,Natalia Prevarskaya,Albrecht Schwab

Journal of biochemical and molecular toxicology 37:e23419 PubMed37341014

2023

Cathepsin S inhibition in dendritic cells prevents Th17 cell differentiation in perivascular adipose tissues following vascular injury in diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyu Peng,Yuan Lv,Changjiang Li,Zichao Cheng,Songyuan He,Cong Wang,Jinghua Liu

Molecular carcinogenesis 62:907-919 PubMed37157950

2023

PCBP1 protects bladder cancer cells from mitochondria injury and ferroptosis by inducing LACTB mRNA degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Luo,Yunli Zhang,Shiyu Pang,Jingxian Min,Tao Wang,Dali Wu,Chun Lin,Zebin Xiao,Qi Xiang,Qing Li,Lili Ma

The Journal of biological chemistry 299:104744 PubMed37100290

2023

Protein-protein interactions in the Mla lipid transport system probed by computational structure prediction and deep mutational scanning.

Applications

Unspecified application

Species

Unspecified reactive species

Mark R MacRae,Dhenesh Puvanendran,Max A B Haase,Nicolas Coudray,Ljuvica Kolich,Cherry Lam,Minkyung Baek,Gira Bhabha,Damian C Ekiert

Clinical, cosmetic and investigational dermatology 16:1063-1070 PubMed37114034

2023

TET2 Promotes Keloid Hyperplasia by Regulating 5hmC Modification in the TGFβ Promoter Region.

Applications

Unspecified application

Species

Unspecified reactive species

Changying Niu,Shenxing Tan

Skeletal muscle 13:4 PubMed36859305

2023

An updated C. elegans nuclear body muscle transcriptome for studies in muscle formation and function.

Applications

Unspecified application

Species

Unspecified reactive species

Anna L Schorr,Alejandro Felix Mejia,Martina Y Miranda,Marco Mangone

Journal of cachexia, sarcopenia and muscle 14:1003-1018 PubMed36864250

2023

Nicotinamide Phosphoribosyltransferase-elevated NAD biosynthesis prevents muscle disuse atrophy by reversing mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Zhang,Yingming Wang,Shuai Lu,Rui Zhong,Zhilin Liu,Qichun Zhao,Chongyang Wang

Science advances 9:eadd6495 PubMed36662867

2023

ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Fäßler,Manjunath G Javoor,Julia Datler,Hermann Döring,Florian W Hofer,Georgi Dimchev,Victor-Valentin Hodirnau,Jan Faix,Klemens Rottner,Florian K M Schur

Clinical science (London, England : 1979) 137:195-217 PubMed36597894

2023

Increased β1-adrenergic receptor antibody confers a vulnerable substrate for atrial fibrillation via mediating Ca2+ mishandling and atrial fibrosis in active immunization rabbit models.

Applications

Unspecified application

Species

Unspecified reactive species

Huaxin Sun,Jie Song,Kai Li,Yao Li,Luxiang Shang,Qina Zhou,Yanmei Lu,Yazhen Zong,Xiuyuan He,Muzappar Kari,Hang Yang,Xianhui Zhou,Ling Zhang,Baopeng Tang
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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