重组Anti-GAPDH抗体[FF26A] (ab59164)
![Western blot - Anti-GAPDH antibody [FF26A] (ab59164)](https://www.abcam.cn/ps/products/59/ab59164/Images/ab59164-390015-AntiGAPDH-antibody-FF26A-F9-western-blot-human.jpg "Western blot - Anti-GAPDH antibody [FF26A] (ab59164)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [FF26A] to GAPDH
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GAPDH抗体[FF26A]
参阅全部 GAPDH 一抗 -
描述
小鼠单克隆抗体[FF26A] to GAPDH -
宿主
Mouse -
经测试应用
适用于: WB, IPmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IP: HepG2 and MDA-MB-231 whole cell lysate. WB: Human brain, heart, kidney and spleen tissue lysate. HeLa, HepG2, Jurkat, A549 and MDA-MB-231 whole cell lysate.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
FF26A -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59164于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 36 kDa.
|
|
| IP |
Use a concentration of 14.7 µg/ml.
|
| 说明 |
|---|
|
WB
1/2000. Predicted molecular weight: 36 kDa. |
|
IP
Use a concentration of 14.7 µg/ml. |
靶标
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功能
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
序列相似性
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
翻译后修饰
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
细胞定位
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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数据库链接
- Entrez Gene: 2597 Human
- Omim: 138400 Human
- SwissProt: P04406 Human
- Unigene: 544577 Human
- Unigene: 592355 Human
- Unigene: 598320 Human
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别名
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
图片
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Western blot - Anti-GAPDH antibody [FF26A] (ab59164)All lanes : Anti-GAPDH antibody [FF26A] (ab59164) at 1/2000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg
Lane 4 : A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 5 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 6 : Human brain tissue lysate at 10 µg
Lane 7 : Human heart tissue lysate at 10 µg
Lane 8 : Human kidney tissue lysate at 10 µg
Lane 9 : Human spleen tissue lysate at 10 µg
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-5: 7 secs; Lanes 6-9: 10 secs.
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![Immunoprecipitation - Anti-GAPDH antibody [FF26A] (ab59164)](https://www.abcam.cn/ps/products/59/ab59164/Images/ab59164-390014-AntiGAPDH-antibody-FF26A-F9-immunoprecipitation-hepg2-human.jpg)
Immunoprecipitation - Anti-GAPDH antibody [FF26A] (ab59164)GADPH was immunoprecipitated from HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab59164 at 14/7µg/ml. Western blot was performed from the immunoprecipitate using ab59164 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.
Lane 1: HepG2 whole cell lysate 10µg (Input).
Lane 2: ab59164 IP in HepG2 whole cell lysate.
Lane 3: Mouse monoclonal IgG instead of ab59164 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 secs. -
![Immunoprecipitation - Anti-GAPDH antibody [FF26A] (ab59164)](https://www.abcam.cn/ps/products/59/ab59164/Images/ab59164-390012-AntiGAPDH-antibody-FF26A-F9-immunoprecipitation-mdamb231-human.jpg)
Immunoprecipitation - Anti-GAPDH antibody [FF26A] (ab59164)GADPH was immunoprecipitated from MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab59164 at 14.7µg/ml. Western blot was performed from the immunoprecipitate using ab59164 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.
Lane 1: MDA-MB-231 whole cell lysate 10µg (Input).
Lane 2: ab59164 IP in MDA-MB-231 whole cell lysate.
Lane 3: Mouse monoclonal IgG instead of ab59164 in MDA-MB-231 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 secs. -
![Anti-GAPDH antibody [FF26A] (ab59164)](https://www.abcam.cn/ps/products/59/ab59164/Images/ab59164-4-benefits-of-recombinant-antibodies.png)
Anti-GAPDH antibody [FF26A] (ab59164)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab59164 被引用在 10 文献中.
- Basu S et al. Rational optimization of a transcription factor activation domain inhibitor. Nat Struct Mol Biol 30:1958-1969 (2023). PubMed: 38049566
- Xie Z et al. C2orf40 inhibits metastasis and regulates chemo-resistance and radio-resistance of nasopharyngeal carcinoma cells by influencing cell cycle and activating the PI3K/AKT/mTOR signaling pathway. J Transl Med 20:264 (2022). PubMed: 35676661
- Tang Y et al. Fatty acid binding protein 5 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by degradation of Krüppel-like factor 9 mediated by miR-889-5p via cAMP-response element binding protein. Cancer Biol Ther 23:424-438 (2022). PubMed: 35816613
- You Y et al. Trypsin is a coordinate regulator of N and P nutrients in marine phytoplankton. Nat Commun 13:4022 (2022). PubMed: 35821503
- Xu S et al. Modulating autophagic flux via ROS-responsive targeted micelles to restore neuronal proteostasis in Alzheimer's disease. Bioact Mater 11:300-316 (2022). PubMed: 34977433
- Dao TNT et al. Chimeric nanocomposites for the rapid and simple isolation of urinary extracellular vesicles. J Extracell Vesicles 11:e12195 (2022). PubMed: 35188341
- Chen C et al. A paclitaxel and microRNA-124 coloaded stepped cleavable nanosystem against triple negative breast cancer. J Nanobiotechnology 19:55 (2021). PubMed: 33632232
- Xu X et al. Bromodomain Containing Protein 4 (BRD4) Regulates Expression of its Interacting Coactivators in the Innate Response to Respiratory Syncytial Virus. Front Mol Biosci 8:728661 (2021). PubMed: 34765643
- Maestre L et al. Expression pattern of XBP1(S) in human B-cell lymphomas. Haematologica 94:419-22 (2009). PubMed: 19176362
- Aparicio T et al. The human GINS complex associates with Cdc45 and MCM and is essential for DNA replication. Nucleic Acids Res 37:2087-95 (2009). PubMed: 19223333