Anti-GAPDH 抗体 [6C5] - Loading Control

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

ab8245 staining GAPDH in SV40LT-SMC (Rat SV40-transfected aorta smooth cell line) cells.

The cells were fixed with 4% formaldehyde (10 minutes) permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1 hour. The cells were then incubated with ab8245 at 5μg/ml and ab202272 at 1/250 dilution overnight at +4°C followed by a further incubation at room temperature for 1h with Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150117) (shown in green). Nuclear DNA was labeled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• WB

Supplier Data

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Blocking and diluting buffer and concentration : Intercept® (TBS) Blocking Buffer diluted with an equal volume of TBS Lysates at 20 µg per lane. The samples were run on a Bis-Tris gel under reducing conditions. Western blot : Anti-NLRP3 antibody (ab283819) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody 6C5 (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab283819 was shown to bind specifically to NLRP3. Target of interest was observed at 118 kDa in untreated wild-type THP-1 cell lysates (lane 1) with no signal observed at this size in NLRP3 knockout cell line ab280063 (knockout cell lysate ab280122) (lane 2). To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.

All lanes:

Western blot - Anti-NLRP3 antibody [RM1021] (<a href='/products/primary-antibodies/nlrp3-antibody-rm1021-ab283819'>ab283819</a>) at 1/1000 dilution

Lane 1:

Wild-type THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 2:

NLRP3 knockout THP-1 whole cell lysate at 20 µg

Secondary

Lanes 1 - 2:

Goat Anti-Rabbit IgG H&L (800CW) at 1/20000 dilution

Lanes 1 - 2:

Goat anti-Mouse IgG H&L (680RD) at 1/20000 dilution

Observed band size: 118 kDa,37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Rabbit Polyclonal to SHMT2/SHMT ab224428 staining at 1/100 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 55 kDa in Wild-type A549 cell lysates with no signal observed at this size in SHMT2 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SHMT2/SHMT antibody (<a href='/products/primary-antibodies/shmt2-shmt-antibody-ab224428'>ab224428</a>) at 1/100 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

Western blot - Human SHMT2 knockout A549 cell line (<a href='/products/cell-lines/human-shmt2-knockout-a549-cell-line-ab301223'>ab301223</a>) at 20 µg

Lane 3:

HeLa at 20 µg

Secondary

Lanes 1 - 3:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 3:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 55 kDa,37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Anti-HK1 antibody staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to HK1. A band was observed at 110 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in HK1 knockout cell line ab262477 (knockout cell lysate ab263918). To generate this image, wild-type and HK1 knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Anti-HK1 antibody at 1/1000 dilution

Lane 1:

Wild-type HEK-293 cell lysate at 20 µg

Lane 2:

Western blot - Human HK1 (Hexokinase 1) knockout HEK-293 cell line (<a href='/products/cell-lines/human-hk1-hexokinase-1-knockout-hek-293-cell-line-ab262477'>ab262477</a>)

Lane 2:

HK1 (Hexokinase 1) knockout HEK-293 cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Predicted band size: 102 kDa

Observed band size: 110 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-TCF12 antibody ab245540 staining at 1/1000 dilution, shown in black; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 80 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in TCF12 knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit HRP (H+L) & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-TCF12 antibody (<a href='/products/primary-antibodies/tcf12-antibody-ab245540'>ab245540</a>) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

Western blot - Human TCF12 knockout U-87 MG cell line (ab306824) at 20 µg

Lane 3:

HeLa at 20 µg

Secondary

Lanes 1 - 3:

Goat anti-Rabbit HRP (H+L) at 1/20000 dilution

Lanes 1 - 3:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 80 kDa

true

Exposure time: 10s

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-Caspase-8 antibody [E6] ab32125 staining at 1/3000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 60 kDa in Wild-type A549 cell lysates with no signal observed at this size in CASP8 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Caspase-8 antibody [E6] (<a href='/products/primary-antibodies/caspase-8-antibody-e6-ab32125'>ab32125</a>) at 1/3000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

Western blot - Human CASP8 knockout A549 cell line (<a href='/products/cell-lines/human-casp8-knockout-a549-cell-line-ab286757'>ab286757</a>) at 20 µg

Lane 3:

Western blot - Human wild-type HCT116 cell line (ab288559) at 20 µg

Lane 4:

Western blot - Human CASP8 knockout HCT116 cell line (<a href='/products/cell-lines/human-casp8-knockout-hct116-cell-line-ab286576'>ab286576</a>) at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 60 kDa,37 kDa

Observed band size: 60 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-RSK1 p90 antibody ab131445 staining at 1/500 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 83 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in RPS6KA1 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-RSK1 p90 antibody (<a href='/products/primary-antibodies/rsk1-p90-antibody-ab131445'>ab131445</a>) at 1/500 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

Western blot - Human RPS6KA1 knockout MCF7 cell line (ab289311) at 20 µg

Lane 3:

PC-3 at 20 µg

Lane 4:

Calu-3 at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa,37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-Caspase-8 antibody [E7] ab32397 staining at 1/500 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 60 kDa in Wild-type A549 cell lysates with no signal observed at this size in CASP8 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Caspase-8 antibody [E7] (<a href='/products/primary-antibodies/caspase-8-antibody-e7-ab32397'>ab32397</a>) at 1/500 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

Western blot - Human CASP8 knockout A549 cell line (<a href='/products/cell-lines/human-casp8-knockout-a549-cell-line-ab286757'>ab286757</a>) at 20 µg

Lane 3:

Western blot - Human wild-type HCT116 cell line (ab288559) at 20 µg

Lane 4:

Western blot - Human CASP8 knockout HCT116 cell line (<a href='/products/cell-lines/human-casp8-knockout-hct116-cell-line-ab286576'>ab286576</a>) at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa,37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-CD58 antibody [EPR24012-147] ab275392 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 55-70 kDa in Wild-type Raji cell lysates with no signal observed at this size in CD58 knockout Raji cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CD58 antibody [EPR24012-147] (<a href='/products/primary-antibodies/cd58-antibody-epr24012-147-ab275392'>ab275392</a>) at 1/1000 dilution

Lane 1:

Wild-type Raji at 20 µg

Lane 2:

Western blot - Human CD58 knockout Raji cell line (<a href='/products/cell-lines/human-cd58-knockout-raji-cell-line-ab290422'>ab290422</a>) at 20 µg

Lane 3:

Wild-type HeLa at 20 µg

Lane 4:

CD58 knockout HeLa cells at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 28 kDa,52-60 kDa

Observed band size: 55-70 kDa,37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-RSK1 p90 antibody [Y81] ab32526 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 83 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in RPS6KA1 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-RSK1 p90 antibody [Y81] (<a href='/products/primary-antibodies/rsk1-p90-antibody-y81-ab32526'>ab32526</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

Western blot - Human RPS6KA1 knockout MCF7 cell line (ab289311) at 20 µg

Lane 3:

PC-3 at 20 µg

Lane 4:

Calu-3 at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa,37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-SHMT2/SHMT antibody [EPR28607-51] ab316328 staining at 1/5000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 55 kDa in Wild-type A549 cell lysates with no signal observed at this size in SHMT2 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SHMT2/SHMT antibody [EPR28607-51] (<a href='/products/primary-antibodies/shmt2-shmt-antibody-epr28607-51-ab316328'>ab316328</a>) at 1/5000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

Western blot - Human SHMT2 knockout A549 cell line (<a href='/products/cell-lines/human-shmt2-knockout-a549-cell-line-ab301223'>ab301223</a>) at 20 µg

Lane 3:

HeLa at 20 µg

Secondary

Lanes 1 - 3:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 3:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 55 kDa,37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-RSK1 p90 antibody [E4] ab32114 staining at 1/500 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 83 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in RPS6KA1 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-RSK1 p90 antibody [E4] (<a href='/products/primary-antibodies/rsk1-p90-antibody-e4-ab32114'>ab32114</a>) at 1/500 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

Western blot - Human RPS6KA1 knockout MCF7 cell line (ab289311) at 20 µg

Lane 3:

PC-3 at 20 µg

Lane 4:

Calu-3 at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa,37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker ab108595 staining at 1/10000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 70/75 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in LMNA knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (<a href='/products/primary-antibodies/lamin-a-lamin-c-antibody-epr4100-nuclear-envelope-marker-ab108595'>ab108595</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

Western blot - Human LMNA knockout MCF7 cell line (ab287603) at 20 µg

Lane 3:

Wild-type HAP1 at 20 µg

Lane 4:

Knockout HAP1 at 20 µg

Lane 5:

HeLa at 20 µg

Secondary

Lanes 1 - 5:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 5:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 74 kDa

Observed band size: 70 kDa,75 kDa,37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-KLF5 antibody [BLR243L] - BSA free ab314109 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 55 kDa in Wild-type HCT 116 cell lysates with no signal observed at this size in KLF5 knockout HCT 116 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (<a href='/products/primary-antibodies/klf5-antibody-blr243l-bsa-free-ab314109'>ab314109</a>) at 1/1000 dilution

Lane 1:

Wild-type HCT 116 at 20 µg

Lane 2:

Western blot - Human KLF5 knockout HCT116 cell line (<a href='/products/cell-lines/human-klf5-knockout-hct116-cell-line-ab301030'>ab301030</a>) at 20 µg

Lane 3:

Western blot - Human wild-type A549 cell line (ab288558) at 20 µg

Lane 4:

Western blot - Human KLF5 knockout A549 cell line (<a href='/products/cell-lines/human-klf5-knockout-a549-cell-line-ab301031'>ab301031</a>) at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 51 kDa,55 kDa

Observed band size: 55 kDa,37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-SLC20A1 antibody staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 70-100 kDa in Wild-type A549 (Phosphate Starved, 6 h) UNBOILED cell lysates with no signal observed at this size in SLC20A1 knockout A549 (Phosphate Starved, 6 h) cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Anti-SLC20A1 antibody at 1/1000 dilution

Lane 1:

Wild-type A549 (Phosphate Starved, 6 h) UNBOILED at 20 µg

Lane 2:

Wild-type A549 (Phosphate Starvation Vehicle Control) UNBOILED at 20 µg

Lane 3:

SLC20A1 knockout A549 (Phosphate Starved, 6 h) UNBOILED at 20 µg

Lane 4:

SLC20A1 knockout A549 (Phosphate Starvation Vehicle Control) UNBOILED at 20 µg

Lane 5:

HEK-293 at 20 µg

Secondary

Lanes 1 - 5:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 5:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 70-100 kDa,36 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Rabbit Monoclonal [EPR14515(2)] to CHD1L ab197019 staining at 1/5000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 110 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in CHD1L knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CHD1L antibody [EPR14515(2)] (<a href='/products/primary-antibodies/chd1l-antibody-epr145152-ab197019'>ab197019</a>) at 1/5000 dilution

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

CHD1L knockout U-87 MG at 20 µg

Lane 3:

Saos-2 at 20 µg

Secondary

Lanes 1 - 3:

Goat anti-Mouse 800CW at 1/20000 dilution

Lanes 1 - 3:

Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 101 kDa

Observed band size: 110 kDa,36 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Rabbit Monoclonal[EPR25059-12] to UBE3A ab290641 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 100 kDa in Wild-type U-87 MG cell lysates with a truncated band observed in UBE3A knockout U-87 MG ab306798 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-UBE3A antibody [EPR25059-12] (<a href='/products/primary-antibodies/ube3a-antibody-epr25059-12-ab290641'>ab290641</a>) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

Western blot - Human UBE3A knockout U-87 MG cell line (<a href='/products/cell-lines/human-ube3a-knockout-u-87-mg-cell-line-ab306798'>ab306798</a>) at 20 µg

Lane 2:

knockout U-87 MG at 20 µg

Lane 3:

Human Brain at 20 µg

Lane 4:

K562 at 20 µg

Lane 5:

T-47D at 20 µg

Secondary

Lanes 1 - 5:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 5:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 101 kDa

Observed band size: 100 kDa,36 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-KLF5 antibody ab137676 staining at 1/2000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 55 kDa in Wild-type HCT 116 cell lysates with no signal observed at this size in KLF5 knockout HCT 116 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-KLF5 antibody (<a href='/products/primary-antibodies/klf5-antibody-ab137676'>ab137676</a>) at 1/2000 dilution

Lane 1:

Wild-type HCT 116 at 20 µg

Lane 2:

Western blot - Human KLF5 knockout HCT116 cell line (<a href='/products/cell-lines/human-klf5-knockout-hct116-cell-line-ab301030'>ab301030</a>) at 20 µg

Lane 3:

Western blot - Human wild-type A549 cell line (ab288558) at 20 µg

Lane 4:

Western blot - Human KLF5 knockout A549 cell line (<a href='/products/cell-lines/human-klf5-knockout-a549-cell-line-ab301031'>ab301031</a>) at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 51 kDa,55 kDa

Observed band size: 55 kDa,37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Rabbit Polyclonal to SHMT2/SHMT ab180786 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 55 kDa in Wild-type A549 cell lysates with no signal observed at this size in SHMT2 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SHMT2/SHMT antibody - C-terminal (<a href='/products/primary-antibodies/shmt2-shmt-antibody-c-terminal-ab180786'>ab180786</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

Western blot - Human SHMT2 knockout A549 cell line (<a href='/products/cell-lines/human-shmt2-knockout-a549-cell-line-ab301223'>ab301223</a>) at 20 µg

Lane 3:

HeLa

Secondary

Lanes 1 - 3:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 3:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 55 kDa,37 kDa

false

• WB

Unknown

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Fluorescence detection of secondary antibody.

All lanes:

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) at 2.5 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) Nuclear at 20 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

A431 (Human epidermoid carcinoma cell line) cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 20 µg

Lane 5:

HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg

Secondary

All lanes:

Alexa Fluor anti-mouse at 1/5000 dilution

Predicted band size: 36 kDa

Observed band size: 37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Anti-LATS1 antibody [C66B5] staining at 1/1000 dilution, shown in black; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab3477S was shown to bind specifically to LATS1. A band was observed at 100-150 kDa in wild-type THP-1 cell lysates with no signal observed at this size in LATS1 knockout cell line. To generate this image, wild-type and LATS1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % BSA in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 20 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Anti-LATS1 antibody at 1/1000 dilution

Lane 1:

Wild-type THP-1 cell lysate at 20 µg

Lane 2:

Western blot - Human LATS1 (WARTS) knockout THP-1 cell line (<a href='/products/cell-lines/human-lats1-warts-knockout-thp-1-cell-line-ab277862'>ab277862</a>)

Lane 2:

LATS1 knockout THP-1 cell lysate at 20 µg

Lane 3:

HT-29 cell lysate at 20 µg

Lane 4:

PC-3 cell lysate at 20 µg

Lane 5:

HeLa cell lysate at 20 µg

Lane 6:

NIH/3T3 cell lysate at 20 µg

Predicted band size: 126 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Rabbit Monoclonal[EPR5108] to HINT1 ab124912 staining at 1/500 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 14 kDa in Wild-type HeLa cell lysates with no signal observed at this size in HINT1 knockout HeLa cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-HINT1 antibody [EPR5108] (<a href='/products/primary-antibodies/hint1-antibody-epr5108-ab124912'>ab124912</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa at 20 µg

Lane 2:

Western blot - Human HINT1 knockout HeLa cell line (<a href='/products/cell-lines/human-hint1-knockout-hela-cell-line-ab265776'>ab265776</a>) at 20 µg

Lane 3:

Jurkat at 20 µg

Lane 4:

HeLa Membrane at 20 µg

Lane 5:

EMPTY

Lane 6:

Western blot - Recombinant Human HINT1 protein (<a href='/products/proteins-peptides/recombinant-human-hint1-protein-ab87362'>ab87362</a>) at 0.1 µg

Secondary

Lanes 1 - 6:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 6:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa,37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Anti-PCAF/KAT2B antibody [EPR2670(N)] (ab176316) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab176316 was found to be non-specific. A band was observed at 75 kDa in wild-type U-2 OS cell lysates with no change observed in the PCAF/KAT2B knockout cell line ab289688 (knockout cell lysate None). To generate this image, wild-type and PCAF/KAT2B knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-KAT2B / PCAF antibody [EPR2670(N)] (<a href='/products/primary-antibodies/kat2b-pcaf-antibody-epr2670n-ab176316'>ab176316</a>) at 1/1000 dilution

Lane 1:

Wild-type U-2 OS cell lysate at 20 µg

Lane 2:

PCAF/KAT2B knockout U-2 OS cell lysate at 20 µg

Lane 3:

DLD-1 cell lysate at 20 µg

Lane 4:

C2C12 cell lysate at 20 µg

Lane 5:

Wild-type HAP1 cell lysate at 20 µg

Lane 6:

KAT2A knockout HAP1 cell lysate at 20 µg

Lane 7:

Human Skeletal Muscle cell lysate at 20 µg

Lane 8:

Human Heart cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 93 kDa

Observed band size: 75 kDa

false

• WB

Supplier Data

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

False colour image of Western blot : Anti-CXCL5 + CXCL6 antibody [EPR22310-196] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab243097 was shown to bind specifically to CXCL5 + CXCL6. A band was observed at 12 kDa in treated wild-type A549 cell lysates with no signal observed at this size in CXCL6 knockout cell line ab275838 (knockout cell lysate ab275812). To generate this image, wild-type and CXCL6 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (<a href='/products/primary-antibodies/cxcl5-cxcl6-antibody-epr22310-196-ab243097'>ab243097</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 Untreated Control cell lysate at 20 µg

Lane 2:

Wild-type A549 Treated TNFa (10 ng/mL, 24 h) + PMA (10 nM, 24 h) cell lysate at 20 µg

Lane 3:

Untreated control at 20 µg

Lane 4:

Treated TNFa (10 ng/mL, 24 h) + PMA (10 nM, 24 h) cell lysate at 20 µg

Lane 5:

Human Lung cell lysate at 20 µg

Lane 6:

MOLT-4 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

False colour image of Western blot : Anti-KPNA2 antibody [EPR25248-95] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab289858 was shown to bind specifically to KPNA2. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-KPNA2 antibody [EPR25248-95] (<a href='/products/primary-antibodies/kpna2-antibody-epr25248-95-ab289858'>ab289858</a>) at 1/1000 dilution

Lane 1:

Human Testis cell lysate at 20 µg

Lane 2:

Human Colon cell lysate at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Lanes 1 - 2:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 57 kDa

Observed band size: 55 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : HRP Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab314407) staining at 1/5000 dilution, shown in black; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab314407 was shown to bind specifically to ACTN2. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibody Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 2 minutes 30 seconds exposure time.

All lanes:

Western blot - HRP Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (<a href='/products/primary-antibodies/hrp-sarcomeric-alpha-actinin-antibody-ep2529y-ab314407'>ab314407</a>) at 1/5000 dilution

Lane 1:

Human skeletal muscle lysate at 20 µg

Lane 2:

Mouse heart lysate at 20 µg

Secondary

All lanes:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 103 kDa

false

• WB

Supplier Data

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

False colour image of Western blot : Anti-CXCL5 antibody [EPR4450(2)] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab126763 was shown to bind specifically to CXCL5. A band was observed at 7 kDa in treated wild-type A549 cell lysates with no signal observed at this size in CXCL5 knockout cell line ab275838 (knockout cell lysate ab275812). To generate this image, wild-type and CXCL5 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-CXCL5 antibody [EPR4450(2)] (<a href='/products/primary-antibodies/cxcl5-antibody-epr44502-ab126763'>ab126763</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 Untreated Control cell lysate at 20 µg

Lane 2:

Wild-type A549 Treated TNFa (10 ng/mL, 24 h) + PMA (10 nM, 24 h) cell lysate at 20 µg

Lane 3:

CXCL5 knockout A549 Untreated Control cell lysate at 20 µg

Lane 4:

CXCL5 knockout A549 Treated TNFa (10 ng/mL, 24 h) + PMA (10 nM, 24 h) cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 7 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Anti-LIFR antibody [EPR24651-109] (ab283651) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab283651 was shown to bind specifically to LIFR. A band was observed at 100-175 kDa in wild-type HeLa cell lysates with no signal observed at this size in LIFR knockout cell line (knockout cell lysate ab282984). To generate this image, wild-type and LIFR knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-LIFR antibody [EPR24651-109] (<a href='/products/primary-antibodies/lifr-antibody-epr24651-109-ab283651'>ab283651</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human LIFR knockout HeLa cell lysate (ab282984)

Lane 2:

LIFR knockout HeLa cell lysate at 20 µg

Lane 3:

PC-3 cell lysate at 20 µg

Lane 4:

T-47D cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 124 kDa

false

• WB

Supplier Data

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Blocking and diluting buffer and concentration : Intercept® (TBS) Blocking Buffer diluted with an equal volume of TBS.

ab315009 can detect both ATG14L and ATG14S isoforms (PMID : 36371383).

In lanes 2 and 4, the lysate was stored at -80℃ prior to Western Blotting. In lanes 1 and 3, lysate was freshly made and used for Western Blotting immediately to minimize protein degradation.

The samples were run on a Bis-Tris gel under reducing conditions.

Anti-ATG14 antibody (ab315009) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody 6C5 (ab8245) loading control staining at 1/20000 dilution, shown in red.

All lanes:

Western blot - Anti-ATG14 antibody [EPR26188-66] (<a href='/products/primary-antibodies/atg14-antibody-epr26188-66-ab315009'>ab315009</a>) at 1/1000 dilution

Lane 1:

Fresh HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Frozen HCT 116 whole cell lysate at 20 µg

Lane 3:

Fresh HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Frozen HepG2 whole cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat Anti-Rabbit IgG H&L (800CW) at 1/20000 dilution

Lanes 1 - 4:

Goat Anti-Mouse IgG H&L (680RD) at 1/20000 dilution

Observed band size: 58 kDa,65 kDa,36 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-LATS1 antibody [EPR23057-116] (ab243656) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab243656 was shown to bind specifically to LATS1. A band was observed at 127 kDa in wild-type THP-1 cell lysates with no signal observed at this size in LATS1 knockout cell line. To generate this image, wild-type and LATS1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-LATS1/WARTS antibody [EPR23057-116] (<a href='/products/primary-antibodies/lats1-warts-antibody-epr23057-116-ab243656'>ab243656</a>) at 1/2000 dilution

Lane 1:

Wild-type THP-1 cell lysate at 20 µg

Lane 2:

Western blot - Human LATS1 (WARTS) knockout THP-1 cell line (<a href='/products/cell-lines/human-lats1-warts-knockout-thp-1-cell-line-ab277862'>ab277862</a>)

Lane 2:

LATS1 knockout THP-1 cell lysate at 20 µg

Lane 3:

HT-29 cell lysate at 20 µg

Lane 4:

PC-3 cell lysate at 20 µg

Lane 5:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 126 kDa

Observed band size: 127 kDa

false

• WB

Supplier Data

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates at 20 µg per lane. The samples were run on a Bis-Tris gel under reducing conditions. Western blot : Anti-AMF antibody (ab313589) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody 6C5 (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab313589 was shown to bind specifically to AMF. Target of interest was observed at 62 kDa in wild-type HEK293T cell lysates (lane 1) with no signal observed at this size in GPI (AMF) knockout cell line ab266834 (knockout cell lysate ab257458) (lane 2). To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged. The identity of the lower MW band at approximately 20 kDa is unknown.

All lanes:

Western blot - Anti-AMF antibody [EPR28372-33] (<a href='/products/primary-antibodies/amf-antibody-epr28372-33-ab313589'>ab313589</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

GPI (AMF) knockout HEK293T whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat Anti-Rabbit IgG H&L (800CW) at 1/20000 dilution

Lanes 1 - 4:

Goat Anti-Mouse IgG H&L (680RD) at 1/20000 dilution

Observed band size: 62 kDa

false

• WB

Supplier Data

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

False colour image of Western blot : Anti-FTO antibody [EPR6894] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab126605 was shown to bind specifically to FTO. A band was observed at 58 kDa in wild-type MCF7 cell lysates with no signal observed at this size in FTO knockout cell line. To generate this image, wild-type and FTO knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-FTO antibody [EPR6894] (<a href='/products/primary-antibodies/fto-antibody-epr6894-ab126605'>ab126605</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 cell lysate at 20 µg

Lane 2:

Western blot - Human FTO knockout MCF7 cell line (<a href='/products/cell-lines/human-fto-knockout-mcf7-cell-line-ab282631'>ab282631</a>)

Lane 2:

FTO knockout MCF7 cell lysate at 20 µg

Lane 3:

HEK-293 cell lysate at 20 µg

Lane 4:

MOLT-4 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

false

• WB

Supplier Data

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

False colour image of Western blot : Anti-PAK2 antibody staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to PAK2. A band was observed at 65 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in PAK2 knockout cell line ab282648 (knockout cell lysate ab283047). The band observed in the knockout lysate lane below 65 kDa is likely to represent a truncated form of PAK2. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and PAK2 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Anti-PAK2 antibody at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human PAK2 knockout HEK-293T cell lysate (ab283047) at 20 µg

Lane 3:

Wild-type HeLa ab255552 cell lysate at 20 µg

Lane 4:

PAK2 knockout HeLa ab260287 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 58 kDa

Observed band size: 65 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Anti-CCL2 antibody [EPR21025] (ab214819) staining at 1/400 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab214819 was shown to bind specifically to CCL2. A band was observed at 13 kDa in wild-type A549 cell lysates with no signal observed at this size in CCL2 knockout cell line ab270478 (knockout cell lysate ab270501). To generate this image, wild-type and CCL2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution and Goat anti-Mouse IgG H&L 680RD at 1/80000 dilution

All lanes:

Western blot - Anti-MCP1 antibody [EPR21025] (<a href='/products/primary-antibodies/mcp1-antibody-epr21025-ab214819'>ab214819</a>)

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Wild-type A549 Treated BFA (1 ug/mL, 3 h) cell lysate, at 20 µg

Lane 3:

CCL2 knockout A549 Treated BFA (1 ug/mL, 3 h) cell lysate at 20 µg

Observed band size: 13 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

False colour image of Western blot : Anti-Daxx antibody [E94] staining at 1/5000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32140 was shown to bind specifically to Daxx. A band was observed at 100 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in DAXX knockout cell line. To generate this image, wild-type and DAXX knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Daxx antibody [E94] (<a href='/products/primary-antibodies/daxx-antibody-e94-ab32140'>ab32140</a>) at 1/5000 dilution

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

Western blot - Human DAXX knockout HCT116 cell line (<a href='/products/cell-lines/human-daxx-knockout-hct116-cell-line-ab287355'>ab287355</a>)

Lane 2:

DAXX knockout HCT 116 cell lysate at 20 µg

Lane 3:

THP-1 cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 81 kDa

Observed band size: 100 kDa

false

• WB

Supplier Data

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

False colour image of Western blot : Anti-RHEB antibody staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to RHEB . A band was observed at 18 kDa in wild-type C2C12 cell lysates with no signal observed at this size in RHEB knockout cell line ab284606. To generate this image, wild-type and RHEB knockout C2C12 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Anti-RHEB antibody at 1/1000 dilution

Lane 1:

Wild-type C2C12 cell lysate at 20 µg

Lane 2:

RHEB knockout C2C12 cell lysate at 20 µg

Lane 3:

Raji cell lysate at 20 µg

Lane 4:

U-2 OS cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 18 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Rabbit Monoclonal [EPR9067(B)] to C1s ab134928 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 80 kDa in Wild-type A549 cell lysates with no signal observed at this size in C1S knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-C1s antibody [EPR9067(B)] (<a href='/products/primary-antibodies/c1s-antibody-epr9067b-ab134928'>ab134928</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

C1S knockout A549 at 20 µg

Lane 3:

HepG2 at 20 µg

Lane 4:

HEK-293 at 20 µg

Lane 5:

Human Liver at 20 µg

Secondary

Lanes 1 - 5:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 5:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 77 kDa,80 kDa

Observed band size: 80 kDa,40 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-SPON1 antibody staining at 1 µg/mL, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 110 kDa in Wild-type OVCAR-3 cell lysates with no signal observed at this size in SPON1 knockout OVCAR-3 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Donkey anti-Goat 800CW & Donkey anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Anti-SPON1 antibody at 1 µg/mL

Lane 1:

Wild-type OVCAR-3 at 20 µg

Lane 2:

Western blot - Human SPON1 knockout NIH:OVCAR-3 cell line (<a href='/products/cell-lines/human-spon1-knockout-nihovcar-3-cell-line-ab308480'>ab308480</a>) at 20 µg

Secondary

Lanes 1 - 2:

Donkey anti-Goat 800CW at 1/20000 dilution

Lanes 1 - 2:

Donkey anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 91 kDa

Observed band size: 110 kDa,37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : PMS2 knockout HeLa cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab110638 observed at 120 kDa. Red - loading control ab8245 observed at 37 kDa.

ab110638 Anti-PMS2 antibody [EPR3947] was shown to specifically react with PMS2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261776 (knockout cell lysate ab257142) was used. Wild-type and PMS2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab110638 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PMS2 antibody [EPR3947] (<a href='/products/primary-antibodies/pms2-antibody-epr3947-ab110638'>ab110638</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PMS2 knockout HeLa cell lysate (<a href='/products/cell-lysates/human-pms2-knockout-hela-cell-lysate-ab257142'>ab257142</a>) at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Lanes 1 - 2:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Predicted band size: 96 kDa

Observed band size: 120 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Anti-PXN antibody [Y113] (ab32084) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32084 was shown to bind specifically to PXN. A band was observed at 70 kDa in wild-type A431 cell lysates with no signal observed at this size in PXN knockout cell line ab261892 (knockout cell lysate ab261701). To generate this image, wild-type and PXN knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

All lanes:

Western blot - Anti-Paxillin antibody [Y113] (<a href='/products/primary-antibodies/paxillin-antibody-y113-ab32084'>ab32084</a>) at 1/1000 dilution

Lane 2:

Western blot - Human PXN (Paxillin) knockout A-431 cell lysate (<a href='/products/cell-lysates/human-pxn-paxillin-knockout-a-431-cell-lysate-ab261701'>ab261701</a>)

Lane 2:

Western blot - Human PXN (Paxillin) knockout A-431 cell line (<a href='/products/cell-lines/human-pxn-paxillin-knockout-a-431-cell-line-ab261892'>ab261892</a>)

Predicted band size: 65 kDa

Observed band size: 70 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-CDH1 antibody [EP700Y] (ab40772) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab40772 was shown to bind specifically to CDH1. A band was observed at 130, 110, 80, 55, 40 kDa in wild-type A431 cell lysates with no signal observed at this size in CDH1 knockout cell line ab273747 (knockout cell lysate ab273781). To generate this image, wild-type and CDH1 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (<a href='/products/primary-antibodies/e-cadherin-antibody-ep700y-intercellular-junction-marker-ab40772'>ab40772</a>) at 1/1000 dilution

Lane 1:

Wild-type A431 cell lysate at 20 µg

Lane 2:

Western blot - Human CDH1 (E Cadherin) knockout A-431 cell lysate (ab273781)

Lane 2:

Western blot - Human CDH1 (E Cadherin) knockout A-431 cell line (<a href='/products/cell-lines/human-cdh1-e-cadherin-knockout-a-431-cell-line-ab273747'>ab273747</a>)

Lane 2:

CDH1 knockout A431 cell lysate at 20 µg

Lane 3:

Caco-2 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 97 kDa

false