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Anti-Ganglioside GD3抗体[R24] (ab11779)

Submit a review Q&A (2)References (23)
Immunocytochemistry/ Immunofluorescence - Anti-Ganglioside GD3 antibody [R24] (ab11779)
  • Immunohistochemistry (Frozen sections) - Anti-Ganglioside GD3 antibody [R24] (ab11779)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ganglioside GD3 antibody [R24] (ab11779)

Key features and details

  • Mouse monoclonal [R24] to Ganglioside GD3
  • Suitable for: IHC-P, ICC/IF, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG3

概述

  • 产品名称

    Anti-Ganglioside GD3抗体[R24]

  • 描述

    小鼠单克隆抗体[R24] to Ganglioside GD3

  • 宿主

    Mouse

  • 经测试应用

    适用于: IHC-P, ICC/IF, IHC-Frmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human Ganglioside GD3. This antibody was raised against SK-MEL-28 melanoma cell line.

  • 阳性对照

    • ICC/IF: SH-SY5Y cells. IHC-P: Human melanoma tissue. IHC-Fr: Human melanoma tisue.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.09% Sodium azide
    Constituents: 98.9% PBS, 1% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 纯化说明

    Affinity purified from tissue culture supernatant.

  • 克隆

    单克隆

  • 克隆编号

    R24

  • 同种型

    IgG3

  • 轻链类型

    kappa

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab11779于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 5 µg/ml.
IHC-Fr
1/40.
说明
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 5 µg/ml.
IHC-Fr
1/40.

靶标

  • 相关性

    Almost all melanomas, astrocytomas, a proportion of sarcomas, a small number of carcinomas, some nevi, as well as normal melanocytes express GD3 antigen. It is one of the most important markers for malignant melanoma. Antibodies to the GD3 ganglioside can induce partial remission of tumor growth in animals as well as in Humans via enhancement of cytotoxic and proliferative response of lymphocytes.

  • 别名

    • GD3 antibody

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Ganglioside GD3 antibody [R24] (ab11779)
    Immunocytochemistry/ Immunofluorescence - Anti-Ganglioside GD3 antibody [R24] (ab11779)
    ICC/IF image of ab11779 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11779, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Frozen sections) - Anti-Ganglioside GD3 antibody [R24] (ab11779)
    Immunohistochemistry (Frozen sections) - Anti-Ganglioside GD3 antibody [R24] (ab11779)

    Frozen section of human melanoma tissue stained for Ganglioside GD3 with ab11779 at 1/40 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ganglioside GD3 antibody [R24] (ab11779)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ganglioside GD3 antibody [R24] (ab11779)
    IHC image of ab11779 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11779, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献 (23)

发表研究结果有使用 ab11779？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab11779 被引用在 23 文献中.

  • Galan A  et al. GD2 and GD3 gangliosides as diagnostic biomarkers for all stages and subtypes of epithelial ovarian cancer. Front Oncol 13:1134763 (2023). PubMed: 37124505
  • Abreu CA  et al. GD3 synthase deletion alters retinal structure and impairs visual function in mice. J Neurochem 158:694-709 (2021). PubMed: 34081777
  • Wang J  et al. Ganglioside GD3 is up-regulated in microglia and regulates phagocytosis following global cerebral ischemia. J Neurochem 158:737-752 (2021). PubMed: 34133773
  • Ramos RI  et al. Upregulation of cell surface GD3 ganglioside phenotype is associated with human melanoma brain metastasis. Mol Oncol 14:1760-1778 (2020). PubMed: 32358995
  • Rosenstock P  et al. Sialylation of Human Natural Killer (NK) Cells is Regulated by IL-2. J Clin Med 9:N/A (2020). PubMed: 32545211
View all Publications for this product

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Dear abcam technical support team: This customer would like to order ab11779 (Anti-Ganglioside GD3 antibody [R24]), she would likes to order this antibody and test in ICC assay with mouse sample, would you please help this customer to create a discount code, the information of this customer as follow:

Could you please help this customer to solve the problem? Thanks for your kindly help.
Best regards

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Answer

I am pleased to hear the customer would like to make use of our testing discount offer and test ab11779 in mouse. I have arranged the following code:

DISCOUNT CODE: #####
Expiration date: ######

This code will give you 1 free primary antibodybefore the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

The terms and conditions applicable to this offer can be found here:

https://www.abcam.com/collaborationdiscounthttps://www.abcam.com/collaborationdiscount.

Any feedback that the customer can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving the Abreview and wish the customer luck with their research.

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Question

What is the concentration of this antibody?

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Answer

Thank you for your enquiry. The concentration of this antibody is 400 ug/ml. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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