重组Anti-GANAB抗体[EPR12376] (ab176349)
![Western blot - Anti-GANAB antibody [EPR12376] (ab176349)](https://www.abcam.cn/ps/products/176/ab176349/Images/ab176349-266650-anti-alpha-glucosidase-ii-antibody-epr12376-western-blot.jpg "Western blot - Anti-GANAB antibody [EPR12376] (ab176349)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12376] to GANAB
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GANAB抗体[EPR12376]
参阅全部 GANAB 一抗 -
描述
兔单克隆抗体[EPR12376] to GANAB -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human GANAB aa 150-250 (Cysteine residue). The exact sequence is proprietary.
Database link: Q14697 -
阳性对照
- HeLa, Human placenta, A431 and HepG2 lysates. Paraffin-embedded Human gastric carcinoma tissue, paraffin-embedded Human placenta tissue, permeabilized Hela cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12376 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176349于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 107 kDa.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/50 - 1/100.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 107 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/50 - 1/100. |
靶标
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功能
Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. -
组织特异性
Detected in placenta. -
通路
Glycan metabolism; N-glycan metabolism. -
序列相似性
Belongs to the glycosyl hydrolase 31 family. -
细胞定位
Endoplasmic reticulum. Golgi apparatus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 23193 Human
- Omim: 104160 Human
- SwissProt: Q14697 Human
- Unigene: 595071 Human
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别名
- Alpha glucosidase II alpha subunit antibody
- Alpha-glucosidase 2 antibody
- G2AN antibody
see all
图片
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Western blot - Anti-GANAB antibody [EPR12376] (ab176349)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GANAB knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab176349 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab176349 was shown to specifically react with GANAB when GANAB knockout samples were used. Wild-type and GANAB knockout samples were subjected to SDS-PAGE. ab176349 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Flow Cytometry (Intracellular) - Anti-GANAB antibody [EPR12376] (ab176349)](https://www.abcam.cn/ps/products/176/ab176349/Images/ab176349-203790-anti-alpha-glucosidase-ii-antibody-epr12376-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-GANAB antibody [EPR12376] (ab176349)Intracellular flow cytometric analysis of permeabilized Hela cells labeling GANAB with ab176349at 1/10 dilution (red)or a rabbit IgG (negative) (green).
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![Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody [EPR12376] (ab176349)](https://www.abcam.cn/ps/products/176/ab176349/Images/ab176349-203789-anti-alpha-glucosidase-ii-antibody-epr12376-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody [EPR12376] (ab176349)Immunofluorescent staining of HeLa cells labeling GANAB with ab176349 at 1/50 dilution (red). DAPI nuclear staining (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody [EPR12376] (ab176349)](https://www.abcam.cn/ps/products/176/ab176349/Images/ab176349-203788-anti-alpha-glucosidase-ii-antibody-epr12376-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody [EPR12376] (ab176349)Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling GANAB with ab176349 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody [EPR12376] (ab176349)](https://www.abcam.cn/ps/products/176/ab176349/Images/ab176349-203787-anti-alpha-glucosidase-ii-antibody-epr12376-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody [EPR12376] (ab176349)Immunohistochemical analysis of paraffin-embedded Humangastric carcinoma tissue labeling GANAB with ab176349 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-GANAB antibody [EPR12376] (ab176349)](https://www.abcam.cn/ps/products/176/ab176349/Images/ab176349-203786-anti-alpha-glucosidase-ii-antibody-epr12376-western-blot.jpg)
Western blot - Anti-GANAB antibody [EPR12376] (ab176349)All lanes : Anti-GANAB antibody [EPR12376] (ab176349) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human placenta lysate
Lane 3 : A431 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 107 kDa -
![Anti-GANAB antibody [EPR12376] (ab176349)](https://www.abcam.cn/ps/products/176/ab176349/Images/ab176349-7-benefits-of-recombinant-antibodies.png)
Anti-GANAB antibody [EPR12376] (ab176349)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab176349 被引用在 2 文献中.
- Wang X et al. Low expression of m6A reader YTHDC1 promotes progression of ovarian cancer via PIK3R1/STAT3/GANAB axis. Int J Biol Sci 19:4672-4688 (2023). PubMed: 37781028
- Lin Q et al. Glycoprotein α-Subunit of Glucosidase II (GIIα) is a novel prognostic biomarker correlated with unfavorable outcome of urothelial carcinoma. BMC Cancer 22:817 (2022). PubMed: 35879690