重组Anti-GAMT抗体[EPR6925] (ab126736)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody [EPR6925] (ab126736)](https://www.abcam.cn/ps/products/126/ab126736/Images/ab126736-140719-anti-gamt-antibody-epr6925-immunohistochemistry.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody [EPR6925] (ab126736)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6925] to GAMT
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GAMT抗体[EPR6925]
参阅全部 GAMT 一抗 -
描述
兔单克隆抗体[EPR6925] to GAMT -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal liver, HepG2 and SH-SY5Y lysates; Human liver tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 7.40 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6925 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126736于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.
靶标
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组织特异性
Expressed in liver. -
通路
Amine and polyamine biosynthesis; creatine biosynthesis; creatine from L-arginine and glycine: step 2/2. -
疾病相关
Defects in GAMT are the cause of guanidinoacetate methyltransferase deficiency (GAMT deficiency) [MIM:612736]. GAMT deficiency is an autosomal recessive disorder characterized by developmental delay/regression, mental retardation, severe disturbance of expressive and cognitive speech, intractable seizures and movement disturbances, severe depletion of creatine/phosphocreatine in the brain, and accumulation of guanidinoacetic acid (GAA) in brain and body fluids. -
序列相似性
Belongs to the RMT2 methyltransferase family. - Information by UniProt
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数据库链接
- Entrez Gene: 2593 Human
- Entrez Gene: 25257 Rat
- Omim: 601240 Human
- SwissProt: Q14353 Human
- SwissProt: P10868 Rat
- Unigene: 81131 Human
- Unigene: 33890 Rat
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别名
- CCDS2 antibody
- Epididymis secretory protein Li 20 antibody
- GAMT antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody [EPR6925] (ab126736)
ab126736 at 1/100 dilution, staining GAMT in Formalin-fixed, Paraffin-embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-GAMT antibody [EPR6925] (ab126736)](https://www.abcam.cn/ps/products/126/ab126736/Images/ab126736-140720-anti-gamt-antibody-epr6925-western-blot.jpg)
Western blot - Anti-GAMT antibody [EPR6925] (ab126736)All lanes : Anti-GAMT antibody [EPR6925] (ab126736) at 1/1000 dilution
Lane 1 : Human fetal liver Lysate
Lane 2 : HepG2 lysate
Lane 3 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa -
![OI-RD Scanning - Anti-GAMT antibody [EPR6925] (ab126736)](https://www.abcam.cn/ps/products/126/ab126736/Images/ab126736-140718-anti-gamt-antibody-epr6925.jpg)
OI-RD Scanning - Anti-GAMT antibody [EPR6925] (ab126736) -
![Anti-GAMT antibody [EPR6925] (ab126736)](https://www.abcam.cn/ps/products/126/ab126736/Images/ab126736-4-benefits-of-recombinant-antibodies.png)
Anti-GAMT antibody [EPR6925] (ab126736)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab126736 被引用在 1 文献中.
- Ryan CR et al. Creatine Monohydrate Supplementation Increases White Adipose Tissue Mitochondrial Markers in Male and Female Rats in a Depot Specific Manner. Nutrients 13:N/A (2021). PubMed: 34371916