Anti-gamma Tubulin抗体(ab11321)
Key features and details
- Rabbit polyclonal to gamma Tubulin
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat, Dog, Human, Chinese hamster
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-gamma Tubulin抗体
参阅全部 gamma Tubulin 一抗 -
描述
兔多克隆抗体to gamma Tubulin -
宿主
Rabbit -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Mouse, Rat, Dog, Human, Chinese hamster -
免疫原
Synthetic peptide:
AATRPDYISWGTQEQ-CG
, with N-terminally added cysteine-glycine, conjugated to KLH, corresponding to amino acids 437-451 of Xenopus laevis gamma Tubulin. -
常规说明
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
纯度
IgG fraction -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11321于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
1/1000.
|
|
| WB | (1) |
1/1000. Predicted molecular weight: 51 kDa.
|
| 说明 |
|---|
|
ICC
1/1000. |
|
WB
1/1000. Predicted molecular weight: 51 kDa. |
靶标
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功能
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. -
序列相似性
Belongs to the tubulin family. -
翻译后修饰
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. -
细胞定位
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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数据库链接
- Entrez Gene: 403695 Dog
- Entrez Gene: 490962 Dog
- Entrez Gene: 27175 Human
- Entrez Gene: 7283 Human
- Entrez Gene: 103733 Mouse
- Entrez Gene: 103768 Mouse
- Entrez Gene: 252921 Rat
- Entrez Gene: 680991 Rat
see all -
别名
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
see all
图片
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Immunocytochemistry - Anti-gamma Tubulin antibody (ab11321)Immunocytochemistry analysis of Rat 1 cells labeling gamma Tubulin with ab11321 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were fixed and permeabilized with methanol followed by acetone.
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Western blot - Anti-gamma Tubulin antibody (ab11321)All lanes : Anti-gamma Tubulin antibody (ab11321) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : NIH-3T3 cell lysate
Lane 3 : MDCK cell lysate
Lane 4 : CHO cell lysate
Lane 5 : Rat-2 cell lysate
Lane 6 : A431 cell lysate
Lane 7 : Jurkat cell lysate
Lane 8 : U87 cell lysate
Lane 9 : C2C12 cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG-peroxidase
Predicted band size: 51 kDa -
Immunocytochemistry - Anti-gamma Tubulin antibody (ab11321)Immunocytochemistry analysis of HeLa cells labeling gamma Tubulin with ab11321 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, Atto-488 conjugate. Cells were fixed and permeabilized with methanol followed by acetone.
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Immunocytochemistry - Anti-gamma Tubulin antibody (ab11321)ICC/IF image of ab11321 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11321, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells. -
Western blot - Anti-gamma Tubulin antibody (ab11321)All lanes : Anti-gamma Tubulin antibody (ab11321) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse hepatocytes whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit (HRP) secondary antibody at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking buffer 5% milk.
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab11321 被引用在 26 文献中.
- Singh A et al. BAP1 loss induces mitotic defects in mesothelioma cells through BRCA1-dependent and independent mechanisms. Oncogene 42:572-585 (2023). PubMed: 36550359
- Cackett G et al. African Swine Fever Virus and Host Response: Transcriptome Profiling of the Georgia 2007/1 Strain and Porcine Macrophages. J Virol 96:e0193921 (2022). PubMed: 35019713
- Castillo P et al. Novel insights linking BRCA1-IRIS role in mammary gland development to formation of aggressive PABCs: the case for longer breastfeeding. Am J Cancer Res 12:396-426 (2022). PubMed: 35141026
- Brodsky IB et al. Divergent Contribution of the Golgi Apparatus to Microtubule Organization in Related Cell Lines. Int J Mol Sci 23:N/A (2022). PubMed: 36555819
- Solic I et al. Expression Pattern of a-Tubulin, Inversin and Its Target Dishevelled-1 and Morphology of Primary Cilia in Normal Human Kidney Development and Diseases. Int J Mol Sci 22:N/A (2021). PubMed: 33800671