Anti-gamma Tubulin抗体(ab11317)
Key features and details
- Rabbit polyclonal to gamma Tubulin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human, Chinese hamster
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-gamma Tubulin抗体
参阅全部 gamma Tubulin 一抗 -
描述
兔多克隆抗体to gamma Tubulin -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human, Chinese hamster
预测可用于: Drosophila melanogaster, Schizosaccharomyces pombe, Sea urchin
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免疫原
Synthetic peptide:
EEFATEGTDRKDVFFY-C
conjugated to KLH, corresponding to N terminal amino acids 38-53 of Human gamma Tubulin -
阳性对照
- ICC/IF: Rat2 cells; NIH3T3 cells; HeLa cells. WB: Rat2 cell lysate; A431 cell lysate; HeLa cell lysate; Jurkat cell lysate; U87 cell lysate; C2C12 cell lysate; CHO cell lysate; NIH3T3 cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
IgG fraction -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11317于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (5) |
1/5000.
It is recommended to fix cells with methanol/acetone. |
| WB | (7) |
1/1000. Detects a band of approximately 48 kDa. This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen.
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| 说明 |
|---|
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ICC/IF
1/5000. It is recommended to fix cells with methanol/acetone. |
|
WB
1/1000. Detects a band of approximately 48 kDa. This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen. |
靶标
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功能
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. -
序列相似性
Belongs to the tubulin family. -
翻译后修饰
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. -
细胞定位
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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数据库链接
- Entrez Gene: 35199 Drosophila melanogaster
- Entrez Gene: 27175 Human
- Entrez Gene: 7283 Human
- Entrez Gene: 103733 Mouse
- Entrez Gene: 103768 Mouse
- Entrez Gene: 252921 Rat
- Entrez Gene: 680991 Rat
- Omim: 191135 Human
see all -
别名
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)
Immunocytochemistry/Immunofluorescence analysis of NIH3T3 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
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Western blot - Anti-gamma Tubulin antibody (ab11317)All lanes : Anti-gamma Tubulin antibody (ab11317) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : C2C12 cell lysate
Lane 5 : Rat2 cell lysate
Lane 6 : U87 cell lysate
Lane 7 : NIH3T3 cell lysate
Lane 8 : CHO cell lysate
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG -
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)Immunocytochemistry/Immunofluorescence analysis of Rat2 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (108)
ab11317 被引用在 108 文献中.
- Muys BR et al. Matrin3 regulates mitotic spindle dynamics by controlling alternative splicing of CDC14B. Cell Rep 42:112260 (2023). PubMed: 36924503
- He S et al. Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 regulate mitotic and male meiotic spindle bipolarity. Nat Commun 14:1715 (2023). PubMed: 36973253
- Chow SE et al. YAP co-localizes with the mitotic spindle and midbody to safeguard mitotic division in lung-cancer cells. FEBS J 290:5704-5719 (2023). PubMed: 37549045
- Esper ME et al. Immunofluorescence Labeling of Skeletal Muscle in Development, Regeneration, and Disease. Methods Mol Biol 2566:113-132 (2023). PubMed: 36152246
- Brand YM et al. A new host-targeted antiviral cyclolignan (SAU-22.107) for Dengue Virus infection in cell cultures. Potential action mechanisms based on cell imaging. Virus Res 323:198995 (2023). PubMed: 36336130