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重组RabMAb

重组Anti-gamma Sarcoglycan抗体[EPR17862-40] (ab203112)

Reviews (1) Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
  • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
  • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
  • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
  • Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
  • Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17862-40] to gamma Sarcoglycan
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-gamma Sarcoglycan抗体[EPR17862-40]
    参阅全部 gamma Sarcoglycan 一抗

  • 描述

    兔单克隆抗体[EPR17862-40] to gamma Sarcoglycan

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IP, IHC-P, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Human skeletal muscle, MCF-7, Human fetal brain, Human fetal heart, Human fetal spleen or Human fetal kidney whole cell lysate. IHC-P: Human skeletal muscle.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR17862-40

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab203112于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
1/50.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
说明
IP
1/50.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).

靶标

  • 功能

    Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

  • 组织特异性

    Expressed in skeletal and heart muscle.

  • 疾病相关

    Defects in SGCG are the cause of limb-girdle muscular dystrophy type 2C (LGMD2C) [MIM:253700]. LGMD2C is characterized by progressive muscle wasting from early childhood.

  • 序列相似性

    Belongs to the sarcoglycan beta/delta/gamma/zeta family.

  • 细胞定位

    Cell membrane > sarcolemma. Cytoplasm > cytoskeleton.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 35 kDa dystrophin associated glycoprotein antibody
      • 35 kDa dystrophin-associated glycoprotein antibody
      • 35DAG antibody
      • 35kD dystrophin associated glycoprotein antibody
      • 35kDa dystrophin-associated glycoprotein antibody
      • A4 antibody
      • DAGA4 antibody
      • DMDA antibody
      • DMDA1 antibody
      • Gamma SG antibody
      • Gamma-sarcoglycan antibody
      • Gamma-SG antibody
      • LGMD2C antibody
      • MAM antibody
      • MGC130048 antibody
      • Sarcoglycan gamma antibody
      • SCARMD2 antibody
      • SCG3 antibody
      • SGCG antibody
      • SGCG_HUMAN antibody
      • TYPE antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)

      Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling gamma Sarcoglycan with ab203112 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cell membrane and weakly cytoplasmic staining on Human skeletal muscle tissue is observed. Counter stained with Hematoxylin.

       

      Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
      Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
      Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112) at 1/10000 dilution + Human skeletal muscle lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 32 kDa
      Observed band size: 32 kDa


      Exposure time: 15 seconds


      Blocking/diluting buffer 5% NFDM/TBST

       

      This protein is expressed in skeletal and heart muscle.

    • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
      Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
      Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112) at 1/2000 dilution + MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µl

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 32 kDa
      Observed band size: 32 kDa


      Exposure time: 3 minutes


      Blcking/Dilution buffer 5% NFDM/TBST

       

      This protein is expressed in skeletal and heart muscle.

    • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
      Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
      All lanes : Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112) at 1/2000 dilution

      Lane 1 : Human fetal brain whole cell lysate
      Lane 2 : Human fetal heart whole cell lysate
      Lane 3 : Human fetal kidney whole cell lysate
      Lane 4 : Human fetal spleen whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 32 kDa
      Observed band size: 32 kDa



      Blocking/DIlution buffer 5% NFDM/TBST.

      ab181602 Anti-GAPDH antibody [EPR16891] used as a control.

      This protein is expressed in skeletal and heart muscle.

    • Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
      Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)

      gamma Sarcoglycan was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab203112 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203112 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Lane 1: Human fetal kidney whole cell lysate10 µg (Input). Lane 2: ab203112 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203112 in Human fetal kidney whole cell lysate.
      Exposure time: 3 seconds

      Blocking/Dilution buffer 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling gamma Sarcoglycan with ab203112 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Counter stained with Hematoxylin. No staining on Human colon tissue is observed.

       

      Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
      Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)

    文献 (1)

    发表研究结果有使用 ab203112？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab203112 被引用在 1 文献中.

    • Israeli D  et al. An AAV-SGCG Dose-Response Study in a ?-Sarcoglycanopathy Mouse Model in the Context of Mechanical Stress. Mol Ther Methods Clin Dev 13:494-502 (2019). PubMed: 31194043

    客户评价及客户问答

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    Immunohistochemistry (Frozen sections) abreview for Anti-gamma Sarcoglycan antibody [EPR17862-40]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Muscle)
    Permeabilization
    No
    Specification
    Muscle
    Blocking step
    (agent) for 30 minute(s) · Concentration: 0.5% · Temperature: 24°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Amir Dori

    Verified customer

    提交于 Mar 14 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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