Anti-galectin 9/Gal-9抗体(ab69630)
Key features and details
- Rabbit polyclonal to galectin 9/Gal-9
- Suitable for: ELISA, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-galectin 9/Gal-9抗体
参阅全部 galectin 9/Gal-9 一抗 -
描述
兔多克隆抗体to galectin 9/Gal-9 -
宿主
Rabbit -
经测试应用
适用于: ELISA, IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human galectin 9/Gal-9 aa 51-100 (internal sequence).
Database link: O00182 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab69630于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
|
| ICC/IF | (1) |
1/500 - 1/1000.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
|
ICC/IF
1/500 - 1/1000. |
靶标
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功能
Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. -
组织特异性
Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue. -
序列相似性
Contains 2 galectin domains. -
结构域
Contains two homologous but distinct carbohydrate-binding domains. -
细胞定位
Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway. - Information by UniProt
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数据库链接
- Entrez Gene: 3965 Human
- Entrez Gene: 16859 Mouse
- Entrez Gene: 25476 Rat
- Omim: 601879 Human
- SwissProt: O00182 Human
- SwissProt: O08573 Mouse
- SwissProt: P97840 Rat
- Unigene: 81337 Human
see all -
别名
- 36 kDa beta-galactoside-binding lectin antibody
- Ecalectin antibody
- Gal-9 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-galectin 9/Gal-9 antibody (ab69630)Immunofluorescence analysis of NIH/3T3 cells using ab69630 at 1/500 dilution, with (right panel) or without (left panel) the immunising peptide.
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Western blot - Anti-galectin 9/Gal-9 antibody (ab69630)All lanes : Anti-galectin 9/Gal-9 antibody (ab69630) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa -
Western blot - Anti-galectin 9/Gal-9 antibody (ab69630)Ab69630 recognizes the partial tagged recombinant Human galectin 9/Gal-9 protein (galectin 1 domain only) which has an expected molecular weight of 19 kDa.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (31)
ab69630 被引用在 31 文献中.
- Wang Y et al. Gal-9/Tim-3 signaling pathway activation suppresses the generation of Th17 cells and promotes the induction of Foxp3+ regulatory T cells in renal ischemia-reperfusion injury. Mol Immunol 156:136-147 (2023). PubMed: 36921488
- Lee M et al. Obesity-induced galectin-9 is a therapeutic target in B-cell acute lymphoblastic leukemia. Nat Commun 13:1157 (2022). PubMed: 35241678
- Grabowska K et al. HIV-1 and methamphetamine alter galectins -1, -3, and -9 in human monocyte-derived macrophages. J Neurovirol 28:99-112 (2022). PubMed: 35175539
- Beyer S et al. Galectin-8 and -9 as prognostic factors for cervical cancer. Arch Gynecol Obstet 306:1211-1220 (2022). PubMed: 35377045
- Wu C et al. Single-cell characterization of malignant phenotypes and microenvironment alteration in retinoblastoma. Cell Death Dis 13:438 (2022). PubMed: 35523772