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Neuroscience Cell Adhesion Proteins Nuclear Proteins

Anti-Galectin 3抗体[A3A12] (ab2785)

  • Datasheet
  • SDS
Reviews (16)Q&A (6)References (74)

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Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

Key features and details

  • Mouse monoclonal [A3A12] to Galectin 3
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Recombinant human Galectin 3 protein (Active) (ab50236)

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概述

  • 产品名称

    Anti-Galectin 3抗体[A3A12]
    参阅全部 Galectin 3 一抗
  • 描述

    小鼠单克隆抗体[A3A12] to Galectin 3
  • 宿主

    Mouse
  • 特异性

    By Western blot, this antibody detects an ~30 kDa protein representing Galectin 3 from Jurkat cells transfected with human Galectin 3. Immunohistochemical staining of Galectin 3 in rat olfactory bulb yields a pattern consistent with nuclear and plasma membrane staining.
  • 经测试应用

    适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein corresponding to Human Galectin 3.

  • 表位

    The epitope for this antibody has been mapped to the first 58 amino acids of Galectin 3.
  • 阳性对照

    • WB: HeLa, MCF7 whole cell lysates. IHC-P: Human ovarian, colon and mouse colon tissue. ICC: HeLa, MCF7 and NIH-3T3 cells.
  • 常规说明

     

    This antibody has been shown to potentiate the binding of Galectin 3 to IgG.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度

    Affinity purified
  • Primary antibody说明

    This antibody has been shown to potentiate the binding of Galectin 3 to IgG.
  • 克隆

    单克隆
  • 克隆编号

    A3A12
  • 同种型

    IgG1
  • 研究领域

    • Neuroscience
    • Cell Adhesion Proteins
    • Nuclear Proteins
    • Cell Biology
    • Cell Cycle
    • Cell differentiation

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human Galectin 3 protein (Active) (ab50236)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2785于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (3)
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
ICC/IF (5)
1/100 - 1/200.
IHC-P (3)
1/20 - 1/200.
说明
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
ICC/IF
1/100 - 1/200.
IHC-P
1/20 - 1/200.

靶标

  • 功能

    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • 组织特异性

    A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • 序列相似性

    Contains 1 galectin domain.
  • 细胞定位

    Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Target information above from: UniProt accession P17931 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3958 Human
    • Entrez Gene: 16854 Mouse
    • Omim: 153619 Human
    • SwissProt: P17931 Human
    • SwissProt: P16110 Mouse
    • Unigene: 531081 Human
    • Unigene: 248615 Mouse
    • 别名

      • 35 kDa lectin antibody
      • Carbohydrate binding protein 35 antibody
      • Carbohydrate-binding protein 35 antibody
      • CBP 35 antibody
      • CBP35 antibody
      • Gal-3 antibody
      • GAL3 antibody
      • Galactose-specific lectin 3 antibody
      • Galactoside-binding protein antibody
      • GALBP antibody
      • Galectin 3 internal gene,included antibody
      • Galectin-3 antibody
      • Galectin3 antibody
      • GALIG antibody
      • GBP antibody
      • IgE binding protein antibody
      • IgE-binding protein antibody
      • L 31 antibody
      • L 34 antibody
      • L-31 antibody
      • L-34 galactoside-binding lectin antibody
      • L31 antibody
      • Laminin-binding protein antibody
      • Lectin L-29 antibody
      • Lectin, galactose binding, soluble 3 antibody
      • LEG3_HUMAN antibody
      • LGALS2 antibody
      • LGALS3 antibody
      • MAC 2 antigen antibody
      • Mac-2 antibody
      • Mac-2 antigen antibody
      • MAC2 antibody
      • Macrophage galactose-specific lectin antibody
      • MGC105387 antibody
      see all

    图片

    • Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
      All lanes : Anti-Galectin 3 antibody [A3A12] (ab2785) at 1/1000 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
      Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
      Lane 4 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
      Lane 5 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
      Lane 6 : A431 (human epidermoid carcinoma cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-Mouse IgG (H+L) (HRP) at 1/4000 dilution

      Developed using the ECL technique.

      Predicted band size: 26 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?
      Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.



      Whole cell extracts were electrophoresed using a 4-12% Bis-Tris Protein Gel. Resolved proteins were then transferred onto a Nitrocellulose membrane using a dry blotting system.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunohistochemical analysis of human ovarian carcinoma tissue (right) labeling Galectin 3 in the nucleus and cytoplasm with ab2785, compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Galectin 3 (green) in the cytoplasm and nucleus with ab2785. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Galectin 3 monoclonal antibody (Product # MA1-940) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x

    • Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
      All lanes : Anti-Galectin 3 antibody [A3A12] (ab2785) at 1/1000 dilution

      Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Predicted band size: 26 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
      All lanes : Anti-Galectin 3 antibody [A3A12] (ab2785) at 1/1000 dilution

      Lane 1 : Wild-Type HeLa (human epithelial cell line from cervix adenocarcinoma) membrane extract
      Lane 2 : HeLa CAS9 membrane extract
      Lane 3 : HeLa LGALS3 knockout membrane extract

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-Mouse IgG (H+L) (HRP) at 1/4000 dilution

      Developed using the ECL technique.

      Predicted band size: 26 kDa



      Knockout of LGALS3 was achieved by CRISPR-Cas9 genome editing using Lentiviral sgRNA and Cas9 Lentivirus. The samples were electrophoresed using 4-12% Bis-Tris Protein Gel. Resolved proteins were then transferred onto a nitrocellulose membrane using a dry blotting system.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunohistochemical analysis (formalin/PFA-fixed paraffin-embedded sections) human colon tissue (right) labelling Galectin 3 in the nucleus and cytoplasm with ab2785, compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunofluorescent analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labelling Galectin 3 (green) in the cytoplasm and nucleus with ab2785. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunohistochemical analysis of (formalin/PFA-fixed paraffin-embedded sections) mouse colon tissue (right) labelling Galectin 3 in the nucleus and cytoplasm with ab2785, compared with a negative control (left) by . To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunofluorescent analysis of MCF7 (Human breast adenocarcinoma cell line) cells labelling Galectin 3 (green) in the cytoplasm and nucleus with ab2785. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (74)

    发表研究结果有使用 ab2785?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2785 被引用在 74 文献中.

    • Tan G  et al. Spleen tyrosine kinase facilitates the progression of papillary thyroid cancer regulated by the hsa_circ_0006417/miR-377-3p axis. Environ Toxicol 39:421-434 (2024). PubMed: 37792549
    • Zhang IY  et al. RAGE ablation attenuates glioma progression and enhances tumor immune responses by suppressing galectin-3 expression. Neuro Oncol 25:886-898 (2023). PubMed: 36394567
    • Zhu L  et al. STAT3/Mitophagy Axis Coordinates Macrophage NLRP3 Inflammasome Activation and Inflammatory Bone Loss. J Bone Miner Res 38:335-353 (2023). PubMed: 36502520
    • Festa LK  et al. Antiretroviral treatment reveals a novel role for lysosomes in oligodendrocyte maturation. J Neurochem 165:722-740 (2023). PubMed: 36718947
    • Deng Y  et al. Elevated galectin-3 levels detected in women with hyperglycemia during early and mid-pregnancy antagonizes high glucose - induced trophoblast cells apoptosis via galectin-3/foxc1 pathway. Mol Med 29:115 (2023). PubMed: 37626284
    View all Publications for this product

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    Western blot abreview for Anti-Galectin 3 antibody [A3A12]

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    Sample
    Rat Cell lysate - whole cell (Rat hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    40 µg
    Specification
    Rat hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Western blot abreview for Anti-Galectin 3 antibody [A3A12]

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    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mice Hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    35 µg
    Specification
    Mice Hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Feb 20 2019

    Western blot abreview for Anti-Galectin 3 antibody [A3A12]

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    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (DU145)
    Gel Running Conditions
    Reduced Denaturing (4-15% SDS PAGE)
    Loading amount
    25 µg
    Specification
    DU145
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Mar 31 2017

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