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重组RabMAb

重组Anti-Galectin 13/PLAC8 + Galectin-16抗体[EPR23383-89] (ab272716)

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Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
  • Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
  • Immunoprecipitation - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
  • Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23383-89] to Galectin 13/PLAC8 + Galectin-16
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Galectin 13/PLAC8 + Galectin-16抗体[EPR23383-89]

  • 描述

    兔单克隆抗体[EPR23383-89] to Galectin 13/PLAC8 + Galectin-16

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, IPmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Human placenta tissue lysate; His-tagged human Galectin 13/PLAC8 recombinant protein; His-lif tagged human Galectin 16 recombinant protein (aa 1-141). IHC-P: Human placenta tissue. IP: Human placenta tissue lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR23383-89

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272716于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
说明
WB
1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.

图片

  • Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    All lanes : Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716) at 1/1000 dilution

    Lane 1 : Human placenta tissue lysate
    Lane 2 : Human skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 16 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 10527825, 19497882).

    Negative control: Human skeletal muscle (PMID: 10527825).

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

  • Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    All lanes : Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716) at 1/1000 dilution

    Lane 1 : His-tagged human Galectin 13/PLAC8 recombinant protein (aa 1-139, ab124309), 20 ng
    Lane 2 : His-lif tagged human Galectin 16 recombinant protein (aa 1-141) 5 µL

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 16 kDa
    Observed band size: 15,55 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This product has cross reaction with Galectin 16.

    Sample loaded onto lane 2 was extract of HEK293 expressing Galectin 16, which contains a lif tag (40 kDa). 

    Exposure time: 70 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Galectin 13+Galectin 16 with ab272716 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placenta (PMID: 25191322). The section was incubated with ab272716 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    Immunoprecipitation - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)

    Galectin 13+Galectin 16 was immunoprecipitated from 0.35 mg human placenta tissue lysate with ab272716 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272716 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

    Lane 1: Human placenta tissue lysate 10µg

    Lane 2: ab272716 IP in human placenta tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272716 in human placenta tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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