Anti-GALE抗体[OTI1C4] (ab118033)
![Western blot - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.cn/ps/products/118/ab118033/Images/ab118033-130015-anti-gale-antibody-oti1c4-western-blot.png "Western blot - Anti-GALE antibody [OTI1C4] (ab118033)")
Key features and details
- Mouse monoclonal [OTI1C4] to GALE
- Suitable for: IHC-P, WB
- Reacts with: Rat, Dog, Human, African green monkey
- Isotype: IgG1
概述
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产品名称
Anti-GALE抗体[OTI1C4]
参阅全部 GALE 一抗 -
描述
小鼠单克隆抗体[OTI1C4] to GALE -
宿主
Mouse -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Rat, Dog, Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human GALE aa 1 to the C-terminus. Produced in HEK-293T cells (NP_000394).
Database link: Q14376 -
阳性对照
- WB: HEK-293T cell lysate transfected with the pCMV6-ENTRY GALE cDNA; HepG2, HeLa, HT-29, A549, COS-7, Jurkat, MDCK, PC-12 and MCF7 cell extracts; Human testis, omentum, uterus, breast, brain, liver, ovary, thyroid gland, colon and spleen extracts. IHC-P: Human breast adenocarcinoma, colon adenocarcinoma, kidney, liver, ovary adenocarcinoma, prostate, prostate carcinoma, bladder carcinoma and lymph node tissues.
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常规说明
The clone number has been updated from 1C4 to OTI1C4, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant. -
克隆
单克隆 -
克隆编号
OTI1C4 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118033于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/500 - 1/2000. Predicted molecular weight: 38 kDa.
|
| 说明 |
|---|
|
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/500 - 1/2000. Predicted molecular weight: 38 kDa. |
靶标
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功能
Catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. -
通路
Carbohydrate metabolism; galactose metabolism. -
疾病相关
Defects in GALE are the cause of epimerase-deficiency galactosemia (EDG) [MIM:230350]; also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation. There are two clinically distinct forms of EDG. (1) A benign, or 'peripheral' form with no detectable GALE activity in red blood cells and characterized by mild symptoms. Some patients may suffer no symptoms beyond raised levels of galactose-1-phosphate in the blood. (2) A much rarer 'generalized' form with undetectable levels of GALE activity in all tissues and resulting in severe features such as restricted growth and mental development. -
序列相似性
Belongs to the sugar epimerase family. - Information by UniProt
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数据库链接
- Entrez Gene: 2582 Human
- Entrez Gene: 114860 Rat
- Omim: 606953 Human
- SwissProt: Q14376 Human
- SwissProt: P18645 Rat
- Unigene: 632380 Human
- Unigene: 29908 Rat
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别名
- FLJ95174 antibody
- FLJ97302 antibody
- Galactose 4 epimerase UDP antibody
see all
图片
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Western blot - Anti-GALE antibody [OTI1C4] (ab118033)All lanes : Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY GALE cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 38 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.cn/ps/products/118/ab118033/Images/ab118033-314129-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human bladder carcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.cn/ps/products/118/ab118033/Images/ab118033-314130-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human lymph node tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Western blot - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.cn/ps/products/118/ab118033/Images/ab118033-314121-anti-gale-antibody-oti1c4-western-blot.jpg)
Western blot - Anti-GALE antibody [OTI1C4] (ab118033)All lanes : Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution
Lane 1 : Human testis extract
Lane 2 : Human omentum extract
Lane 3 : Human uterus extract
Lane 4 : Human breast extract
Lane 5 : Human brain extract
Lane 6 : Human liver extract
Lane 7 : Human ovary extract
Lane 8 : Human thyroid gland extract
Lane 9 : Human colon extract
Lane 10 : Human spleen extract
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.cn/ps/products/118/ab118033/Images/ab118033-314128-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human prostate carcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.cn/ps/products/118/ab118033/Images/ab118033-314127-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human prostate tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.cn/ps/products/118/ab118033/Images/ab118033-314126-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human ovary adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.cn/ps/products/118/ab118033/Images/ab118033-314125-ab1180333141250335.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human liver tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.cn/ps/products/118/ab118033/Images/ab118033-314124-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human kidney tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.cn/ps/products/118/ab118033/Images/ab118033-314123-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human colon adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.cn/ps/products/118/ab118033/Images/ab118033-314122-anti-gale-antibody-oti1c4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)Paraffin-embedded human breast adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.
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![Western blot - Anti-GALE antibody [OTI1C4] (ab118033)](https://www.abcam.cn/ps/products/118/ab118033/Images/ab118033-130014-anti-gale-antibody-oti1c4-western-blot.jpg)
Western blot - Anti-GALE antibody [OTI1C4] (ab118033)All lanes : Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (Canine kidney cell line) cell extract
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 38 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab118033 被引用在 1 文献中.
- Broussard A et al. Human UDP-galactose 4'-epimerase (GALE) is required for cell-surface glycome structure and function. J Biol Chem 295:1225-1239 (2020). PubMed: 31819007