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AB118033

Anti-GALE抗体[OTI1C4]

Anti-GALE antibody [OTI1C4]

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(2 Publications)

Mouse Monoclonal GALE antibody. Suitable for IHC-P, WB and reacts with Human, African green monkey, Dog, Rat, Transfected cell lysate samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GALE.

查看别名

UDP-glucose 4-epimerase, Galactowaldenase, UDP-N-acetylgalactosamine 4-epimerase, UDP-N-acetylglucosamine 4-epimerase, UDP-galactose 4-epimerase, UDP-GalNAc 4-epimerase, UDP-GlcNAc 4-epimerase, GALE

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human lymph node tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human bladder carcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human prostate carcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human prostate tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human breast adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human liver tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human kidney tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human ovary adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human colon adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

Western blot - Anti-GALE antibody [OTI1C4] (AB118033)
  • WB

Unknown

Western blot - Anti-GALE antibody [OTI1C4] (AB118033)

All lanes:

Western blot - Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

HT-29 (Human colorectal adenocarcinoma cell line) cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (african green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (Canine kidney cell line) cell extract at 35 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (Human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 38 kDa

false

Western blot - Anti-GALE antibody [OTI1C4] (AB118033)
  • WB

Supplier Data

Western blot - Anti-GALE antibody [OTI1C4] (AB118033)

All lanes:

Western blot - Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution

Lane 1:

Human testis extract at 10 µg

Lane 2:

Human omentum extract at 10 µg

Lane 3:

Human uterus extract at 10 µg

Lane 4:

Human breast extract at 10 µg

Lane 5:

Human brain extract at 10 µg

Lane 6:

Human liver extract at 10 µg

Lane 7:

Human ovary extract at 10 µg

Lane 8:

Human thyroid gland extract at 10 µg

Lane 9:

Human colon extract at 10 µg

Lane 10:

Human spleen extract at 10 µg

Predicted band size: 38 kDa

false

Western blot - Anti-GALE antibody [OTI1C4] (AB118033)
  • WB

Unknown

Western blot - Anti-GALE antibody [OTI1C4] (AB118033)

All lanes:

Western blot - Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY GALE cDNA at 5 µg

Predicted band size: 38 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

OTI1C4

亚型

IgG1

不含载体蛋白

No

反应种属

Rat, Dog, Human, African green monkey

应用

WB, IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human GALE.

Q14376

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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产品详情

The clone number has been updated from 1C4 to OTI1C4, both clone numbers name the same clone.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
纯化说明
Purified from cell culture supernatant.
存储溶液
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

'GALE' also known as UDP-galactose-4-epimerase is an enzyme that plays a critical role in carbohydrate metabolism. It weighs approximately 38 kDa and is expressed in various tissues across the body. This enzyme is responsible for the interconversion of UDP-galactose and UDP-glucose essential in the Leloir pathway of galactose metabolism. GALE's activity is necessary for maintaining balanced sugar levels and genetic mutations in this protein can lead to significant metabolic issues.
Biological function summary

UDP-galactose-4-epimerase facilitates the conversion of galactose-1-phosphate to UDP-galactose a reaction occurring in the utilization of galactose obtained from diet. This enzyme is not part of a larger protein complex but operates independently to mediate the transformation of sugars. Its activity helps in the synthesis of glycoproteins and glycolipids which are fundamental to various cellular functions including cell signaling and adhesion.

Pathways

GALE is an important component in the galactose metabolism pathway. It ensures the proper function of carbohydrate interconversion processes important for energy production and biomolecule synthesis. GALE interrelates with other enzymes like GALT (galactose-1-phosphate uridyltransferase) and GALK1 (galactokinase 1) which together facilitate the orderly breakdown and use of galactose in cells. Disruption in its function can affect these interconnected pathways leading to metabolic imbalances.

Deficiencies or mutations in GALE lead to type III galactosemia which is characterized by the body's inability to properly manage galactose. This genetic disorder can cause symptoms such as jaundice cataracts and developmental delays. GALE's relationship with GALT another enzyme implicated in classic galactosemia (type I) highlights an interconnected network where disruptions in similar pathways can result in metabolic dysfunctions. Careful monitoring of these enzymes is necessary in managing and understanding the metabolic disorders tied to galactose metabolism.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Catalyzes two distinct but analogous reactions : the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.
See full target information GALE
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 295:1225-1239 PubMed31819007

2019

Human UDP-galactose 4'-epimerase (GALE) is required for cell-surface glycome structure and function.

Applications

Unspecified application

Species

Unspecified reactive species

Alex Broussard,Alyssa Florwick,Chelsea Desbiens,Nicole Nischan,Corrina Robertson,Ziqiang Guan,Jennifer J Kohler,Lance Wells,Michael Boyce

Cell cycle (Georgetown, Tex.) 11:4174-80 PubMed23070475

2012

Mitochondrial biogenesis in epithelial cancer cells promotes breast cancer tumor growth and confers autophagy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed F Salem,Diana Whitaker-Menezes,Anthony Howell,Federica Sotgia,Michael P Lisanti
View all publications
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