AB155997

重组Anti-GALE抗体[EPR11088(B)]

Anti-GALE antibody [EPR11088(B)]

RabMAb
Recombinant
了解详情

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(3 Publications)

Rabbit Recombinant Monoclonal GALE antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

查看别名

UDP-glucose 4-epimerase, Galactowaldenase, UDP-N-acetylgalactosamine 4-epimerase, UDP-N-acetylglucosamine 4-epimerase, UDP-galactose 4-epimerase, UDP-GalNAc 4-epimerase, UDP-GlcNAc 4-epimerase, GALE

2 Images

Flow Cytometry (Intracellular) - Anti-GALE antibody [EPR11088(B)] (AB155997)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-GALE antibody [EPR11088(B)] (AB155997)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling GALE with ab155997 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

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Western blot - Anti-GALE antibody [EPR11088(B)] (AB155997)

All lanes:

Western blot - Anti-GALE antibody [EPR11088(B)] (ab155997) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 38 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-GALE antibody [EPR11088(B)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11088(B)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

'GALE' also known as UDP-galactose-4-epimerase is an enzyme that plays a critical role in carbohydrate metabolism. It weighs approximately 38 kDa and is expressed in various tissues across the body. This enzyme is responsible for the interconversion of UDP-galactose and UDP-glucose essential in the Leloir pathway of galactose metabolism. GALE's activity is necessary for maintaining balanced sugar levels and genetic mutations in this protein can lead to significant metabolic issues.

Biological function summary

UDP-galactose-4-epimerase facilitates the conversion of galactose-1-phosphate to UDP-galactose a reaction occurring in the utilization of galactose obtained from diet. This enzyme is not part of a larger protein complex but operates independently to mediate the transformation of sugars. Its activity helps in the synthesis of glycoproteins and glycolipids which are fundamental to various cellular functions including cell signaling and adhesion.

Pathways

GALE is an important component in the galactose metabolism pathway. It ensures the proper function of carbohydrate interconversion processes important for energy production and biomolecule synthesis. GALE interrelates with other enzymes like GALT (galactose-1-phosphate uridyltransferase) and GALK1 (galactokinase 1) which together facilitate the orderly breakdown and use of galactose in cells. Disruption in its function can affect these interconnected pathways leading to metabolic imbalances.

Deficiencies or mutations in GALE lead to type III galactosemia which is characterized by the body's inability to properly manage galactose. This genetic disorder can cause symptoms such as jaundice cataracts and developmental delays. GALE's relationship with GALT another enzyme implicated in classic galactosemia (type I) highlights an interconnected network where disruptions in similar pathways can result in metabolic dysfunctions. Careful monitoring of these enzymes is necessary in managing and understanding the metabolic disorders tied to galactose metabolism.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes two distinct but analogous reactions : the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.

See full target information GALE

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:1771 PubMed32286306

2020

Chronic activation of hexosamine biosynthesis in the heart triggers pathological cardiac remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Diem Hong Tran,Herman I May,Qinfeng Li,Xiang Luo,Jian Huang,Guangyu Zhang,Erica Niewold,Xiaoding Wang,Thomas G Gillette,Yingfeng Deng,Zhao V Wang

Diabetes 66:2789-2799 PubMed28877911

2017

Hepatic GALE Regulates Whole-Body Glucose Homeostasis by Modulating Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhu,Shangang Zhao,Yingfeng Deng,Ruth Gordillo,Alexandra L Ghaben,Mengle Shao,Fang Zhang,Ping Xu,Yang Li,Huachuan Cao,Olga Zagnitko,David A Scott,Rana K Gupta,Chao Xing,Bei B Zhang,Hua V Lin,Philipp E Scherer

ACS chemical biology 12:2030-2039 PubMed28541657

2017

Chemical Modulation of Protein O-GlcNAcylation via OGT Inhibition Promotes Human Neural Cell Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Lissette M Andres,Ian W Blong,Angela C Evans,Neil G Rumachik,Teppei Yamaguchi,Nam D Pham,Pamela Thompson,Jennifer J Kohler,Carolyn R Bertozzi

View all publications

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重组Anti-GALE抗体[EPR11088(B)]