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AB175185

重组Anti-GAL4抗体[EPR12710(B)]

Anti-GAL4 antibody [EPR12710(B)]

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(3 Publications)

Rabbit Recombinant Monoclonal GAL4 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 3 publications.

查看别名

Galectin-4, Gal-4, Antigen NY-CO-27, L-36 lactose-binding protein, Lactose-binding lectin 4, L36LBP, LGALS4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (AB175185)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (AB175185)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling GAL4 with ab175185 at a 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (AB175185)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (AB175185)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling GAL4 with ab175185 at a 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-GAL4 antibody [EPR12710(B)] (AB175185)
  • WB

Supplier Data

Western blot - Anti-GAL4 antibody [EPR12710(B)] (AB175185)

All lanes:

Western blot - Anti-GAL4 antibody [EPR12710(B)] (ab175185) at 1/1000 dilution

Lane 1:

HT-29 cell lysates at 10 µg

Lane 2:

Human small intestine lysates at 10 µg

Lane 3:

Rat small intestine lysates at 10 µg

Predicted band size: 36 kDa

false

不同偶联物与剂型 (1)

  • Carrier free

    Anti-GAL4 antibody [EPR12710(B)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12710(B)

亚型

IgG

不含载体蛋白

No

反应种属

Rat, Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Conditional Ambient
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
See full target information Galectin-4

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Bioengineering & translational medicine 10:e70021 PubMed41030277

2025

Engineered lung cell targeting and SLC7A11 siRNA expressing bacterial extracellular vesicles impair the progression of none-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Dan Wan,Xue-Liang Zhou,Jin-Long Liu,Hua Xu

Nature communications 13:7578 PubMed36481721

2022

Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Chen,Chunyu Yu,Xinhua Liu,Beibei Liu,Xiaodi Wu,Jiajing Wu,Dong Yan,Lulu Han,Zifan Tang,Xinyi Yuan,Jianqiu Wang,Yue Wang,Shumeng Liu,Lin Shan,Yongfeng Shang

The European journal of neuroscience 44:2226-35 PubMed27306299

2016

Brain BDNF levels are dependent on cerebrovascular endothelium-derived nitric oxide.

Applications

Unspecified application

Species

Unspecified reactive species

Hayat Banoujaafar,Alice Monnier,Nicolas Pernet,Aurore Quirié,Philippe Garnier,Anne Prigent-Tessier,Christine Marie
View all publications

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