Anti-GADD34抗体(ab9869)
Key features and details
- Goat polyclonal to GADD34
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-GADD34抗体
参阅全部 GADD34 一抗 -
描述
山羊多克隆抗体to GADD34 -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human GADD34 aa 650 to the C-terminus (C terminal) (Cysteine residue). NP_055145.3.
Database link: O75807-1 -
阳性对照
- WB: HepG2 cell lysate. IHC-P: Human liver tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB | (1) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. -
序列相似性
Belongs to the PPP1R15 family. -
翻译后修饰
Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity. -
细胞定位
Endoplasmic reticulum. - Information by UniProt
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数据库链接
- Entrez Gene: 23645 Human
- Entrez Gene: 17872 Mouse
- Omim: 611048 Human
- SwissProt: O75807 Human
- SwissProt: P17564 Mouse
- Unigene: 631593 Human
- Unigene: 4048 Mouse
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别名
- Apoptosis associated protein antibody
- GADD 34 antibody
- Growth arrest and DNA damage inducible 34 antibody
see all
图片
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Western blot - Anti-GADD34 antibody (ab9869)Anti-GADD34 antibody (ab9869) at 0.1 µg/ml + HEPG2 cell lysate at 35 µg
Predicted band size: 75 kDa
Primary incubation for 1 hour. Detection by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GADD34 antibody (ab9869)IHC image of ab9869 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9869, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GADD34 antibody (ab9869)ab9869 staining GADD34 in human liver sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
数据表及文件
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Datasheet download
文献 (10)
ab9869 被引用在 10 文献中.
- Li X et al. Oleandrin, a cardiac glycoside, induces immunogenic cell death via the PERK/elF2a/ATF4/CHOP pathway in breast cancer. Cell Death Dis 12:314 (2021). PubMed: 33762577
- Yuan T et al. miR-195 promotes LPS-mediated intestinal epithelial cell apoptosis via targeting SIRT1/eIF2a. Int J Mol Med 45:510-518 (2020). PubMed: 31894250
- Hou X et al. Galanthamine improves myocardial ischemia-reperfusion-induced cardiac dysfunction, endoplasmic reticulum stress-related apoptosis, and myocardial fibrosis by suppressing AMPK/Nrf2 pathway in rats. Ann Transl Med 7:634 (2019). PubMed: 31930035
- Li YH et al. Halofuginone protects against advanced glycation end products-induced injury of H9C2 cells via alleviating endoplasmic reticulum stress-associated apoptosis and inducing autophagy. Mol Med Rep 20:3131-3139 (2019). PubMed: 31432112
- Bahar E et al. Polyphenolic Extract of Euphorbia supina Attenuates Manganese-Induced Neurotoxicity by Enhancing Antioxidant Activity through Regulation of ER Stress and ER Stress-Mediated Apoptosis. Int J Mol Sci 18:N/A (2017). PubMed: 28146110
- Galindo I et al. The ATF6 branch of unfolded protein response and apoptosis are activated to promote African swine fever virus infection. Cell Death Dis 3:e341 (2012). WB . PubMed: 22764100
- Garrido JL et al. EBNA3C interacts with Gadd34 and counteracts the unfolded protein response. Virol J 6:231 (2009). PubMed: 20040105
- Kepp O et al. Disruption of the PP1/GADD34 complex induces calreticulin exposure. Cell Cycle 8:3971-7 (2009). PubMed: 19901557
- Panaretakis T et al. Mechanisms of pre-apoptotic calreticulin exposure in immunogenic cell death. EMBO J 28:578-90 (2009). WB ; Mouse . PubMed: 19165151
- Criollo A et al. Regulation of autophagy by the inositol trisphosphate receptor. Cell Death Differ 14:1029-39 (2007). WB ; Human . PubMed: 17256008
