AB9869

Anti-GADD34抗体

Name: GADD34抗体 (ab9869)| Abcam中文官网
Brand: Abcam Limited
SKU: AB9869
Price: 4807 CNY
Availability: InStock
Rating: 3 (1 reviews)

Anti-GADD34 antibody

BOND RX™ Validated
了解详情

(1 Review)

(12 Publications)

Goat Polyclonal GADD34 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human PPP1R15A aa 650 to C-terminus.

查看别名

GADD34, PPP1R15A, Protein phosphatase 1 regulatory subunit 15A, Growth arrest and DNA damage-inducible protein GADD34, Myeloid differentiation primary response protein MyD116 homolog

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GADD34 antibody (AB9869)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GADD34 antibody (AB9869)

IHC image of ab9869 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9869, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GADD34 antibody (AB9869)

ab9869 staining GADD34 in human liver sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Supplier Data

Western blot - Anti-GADD34 antibody (AB9869)

Primary incubation for 1 hour. Detection by chemiluminescence.

All lanes:

Western blot - Anti-GADD34 antibody (ab9869) at 0.1 µg/mL

All lanes:

HEPG2 cell lysate at 35 µg

Predicted band size: 73 kDa

false

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human, Mouse

应用

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human PPP1R15A aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75807

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Recruits the serine/threonine-protein phosphatase PPP1CA to prevents excessive phosphorylation of the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress (PubMed : 26095357, PubMed : 26742780). Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1 (PubMed : 14718519). May promote apoptosis by inducing p53/TP53 phosphorylation on 'Ser-15' (PubMed : 14635196). Plays an essential role in autophagy by tuning translation during starvation, thus enabling lysosomal biogenesis and a sustained autophagic flux (PubMed : 32978159). Also acts a viral restriction factor by attenuating HIV-1 replication (PubMed : 31778897). Mechanistically, mediates the inhibition of HIV-1 TAR RNA-mediated translation (PubMed : 31778897).. (Microbial infection) Promotes enterovirus 71 replication by mediating the internal ribosome entry site (IRES) activity of viral 5'-UTR.

See full target information PPP1R15A

style

left-column

文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:410 PubMed38866787

2024

Human gastric cancer progression and stabilization of ATG2B through RNF5 binding facilitated by autophagy-associated CircDHX8.

Applications

Unspecified application

Species

Unspecified reactive species

Guanxin Wei,Xiang Chen,Tuo Ruan,Xianxiong Ma,Xiuxian Zhu,Wenhao Wen,Danzeng He,Kaixiong Tao,Chuanqing Wu

Scientific reports 11:11893 PubMed34088951

2021

Microarray analysis reveals ONC201 mediated differential mechanisms of CHOP gene regulation in metastatic and nonmetastatic colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ashraf Al Madhoun,Dania Haddad,Mustafa Al Tarrah,Sindhu Jacob,Waleed Al-Ali,Rasheeba Nizam,Lavina Miranda,Fatema Al-Rashed,Sardar Sindhu,Rasheed Ahmad,Milad S Bitar,Fahd Al-Mulla

Cell death & disease 12:314 PubMed33762577

2021

Oleandrin, a cardiac glycoside, induces immunogenic cell death via the PERK/elF2α/ATF4/CHOP pathway in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxi Li,Jian Zheng,Shi Chen,Fan-Dong Meng,Jing Ning,Shu-Lan Sun

Annals of translational medicine 7:634 PubMed31930035

2020

Galanthamine improves myocardial ischemia-reperfusion-induced cardiac dysfunction, endoplasmic reticulum stress-related apoptosis, and myocardial fibrosis by suppressing AMPK/Nrf2 pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolin Hou,Minhuan Fu,Biao Cheng,Yu Kang,Dili Xie

International journal of molecular medicine 45:510-518 PubMed31894250

2020

miR‑195 promotes LPS‑mediated intestinal epithelial cell apoptosis via targeting SIRT1/eIF2a.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Yuan,Li Zhang,Shuo Yao,Shuang-Ya Deng,Ji-Qiang Liu

Molecular medicine reports 20:3131-3139 PubMed31432112

2019

Halofuginone protects against advanced glycation end products‑induced injury of H9C2 cells via alleviating endoplasmic reticulum stress‑associated apoptosis and inducing autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hui Li,Wei-Li Zhang,Hao-Ying Zhou,Da-Wei Yu,Xiao-Ning Sun,Qin Hu

International journal of molecular sciences 18: PubMed28146110

2017

Polyphenolic Extract of Euphorbia supina Attenuates Manganese-Induced Neurotoxicity by Enhancing Antioxidant Activity through Regulation of ER Stress and ER Stress-Mediated Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Entaz Bahar,Geum-Hwa Lee,Kashi Raj Bhattarai,Hwa-Young Lee,Min-Kyung Choi,Harun-Or Rashid,Ji-Ye Kim,Han-Jung Chae,Hyonok Yoon

Cell death & disease 3:e341 PubMed22764100

2012

The ATF6 branch of unfolded protein response and apoptosis are activated to promote African swine fever virus infection.

Applications

Species

Unspecified reactive species

I Galindo,B Hernáez,R Muñoz-Moreno,M A Cuesta-Geijo,I Dalmau-Mena,C Alonso

Virology journal 6:231 PubMed20040105

2009

EBNA3C interacts with Gadd34 and counteracts the unfolded protein response.

Applications

Unspecified application

Species

Unspecified reactive species

Jose L Garrido,Seijii Maruo,Kenzo Takada,Adam Rosendorff

Cell cycle (Georgetown, Tex.) 8:3971-7 PubMed19901557

2009

Disruption of the PP1/GADD34 complex induces calreticulin exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Oliver Kepp,Lorenzo Galluzzi,Fabrizio Giordanetto,Antoine Tesniere,Ilio Vitale,Isabelle Martins,Frederic Schlemmer,Sandy Adjemian,Laurence Zitvogel,Guido Kroemer

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GADD34抗体

Anti-GADD34 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GADD34 antibody (AB9869)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GADD34 antibody (AB9869)

Western blot - Anti-GADD34 antibody (AB9869)