Anti-GADD34抗体(ab175355)
Key features and details
- Rabbit polyclonal to GADD34
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-GADD34抗体
参阅全部 GADD34 一抗 -
描述
兔多克隆抗体to GADD34 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human GADD34 aa 595-612.
Sequence:RITQAQEELSPCLTPAAR
Database link: O75807 -
阳性对照
- WB: HeLa cell lysate. ICC: HeLa cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175355于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/250 - 1/1000. Predicted molecular weight: 73 kDa.
|
|
| ICC/IF |
1/50 - 1/100.
|
| 说明 |
|---|
|
WB
1/250 - 1/1000. Predicted molecular weight: 73 kDa. |
|
ICC/IF
1/50 - 1/100. |
靶标
-
功能
Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. -
序列相似性
Belongs to the PPP1R15 family. -
翻译后修饰
Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity. -
细胞定位
Endoplasmic reticulum. - Information by UniProt
-
数据库链接
- Entrez Gene: 23645 Human
- Omim: 611048 Human
- SwissProt: O75807 Human
- Unigene: 631593 Human
-
别名
- Apoptosis associated protein antibody
- GADD 34 antibody
- Growth arrest and DNA damage inducible 34 antibody
see all
图片
-
Western blot - Anti-GADD34 antibody (ab175355)Lane 2 : Anti-GADD34 antibody (ab175355) at 1/250 dilution
Lanes 3 & 5 : Anti-GADD34 antibody (ab175355) at 1/500 dilution
Lane 4 : Anti-GADD34 antibody (ab175355) at 1/1000 dilution
Lanes 2-4 : HeLa cell lysate
Lane 5 : HeLa cell lysate with neutralizing peptide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP secondary antibody
Developed using the ECL technique.
Predicted band size: 73 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-GADD34 antibody (ab175355)Immunofluorescent analysis of formalin-fixed HeLa cells labeling GADD34 with ab175355 at 1/50 and incubated with DyLight 488 Goat anti-rabbit IgG secondary antibody. F-actin (red) was stained with DyLight 650 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab175355 尚未被引用在任何文献中。