Anti-GAD67/GAD1 抗体 [EPR20578]

• IP

Supplier Data

Immunoprecipitation - Anti-GAD67/GAD1 antibody [EPR20578] (AB213508)

GAD67 was immunoprecipitated from 0.35 mg of human brain lysate with ab213508 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213508 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : Human brain lysate 10 μg (Input).

Lane 2 : ab213508 IP in human brain lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab213508 in human brain lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-GAD67/GAD1 antibody [EPR20578] (ab213508)

Predicted band size: 66 kDa

false

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 antibody [EPR20578] (AB213508)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat midbrain neuron cells labelling GAD67 with ab213508 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing positive staining in rat midbrain neuron cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 μg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 μg/mL) (Red). The nuclear counterstain was DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [EPR20578] (AB213508)

Immunohistochemical analysis of paraffin-embedded rat cerebellar cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat cerebellar cortex (PMID : 17235515). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [EPR20578] (AB213508)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on islet of mouse pancreas (PMID : 8243826). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [EPR20578] (AB213508)

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cerebral cortex (PMID : 25904362). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [EPR20578] (AB213508)

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat cerebral cortex (PMID : 25904362). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [EPR20578] (AB213508)

Immunohistochemical analysis of paraffin-embedded mouse cerebellar cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cerebellar cortex (PMID : 17235515). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [EPR20578] (AB213508)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on islet of rat pancreas (PMID : 8243826). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-GAD67/GAD1 antibody [EPR20578] (AB213508)

Blocking/Dilution buffer : 5% NFDM/TBST.

Negative control : Human lung (PMID : 10671565).

All lanes:

Western blot - Anti-GAD67/GAD1 antibody [EPR20578] (ab213508) at 1/1000 dilution

Lane 1:

Human cerebellum lysate at 20 µg

Lane 2:

Human fetal brain lysate at 20 µg

Lane 3:

Human lung lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 66 kDa

Observed band size: 67 kDa

true

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-GAD67/GAD1 antibody [EPR20578] (AB213508)

Blocking/Dilution buffer : 5% NFDM/TBST.

Negative control : Mouse lung, rat lung (PMID : 10671565).

All lanes:

Western blot - Anti-GAD67/GAD1 antibody [EPR20578] (ab213508) at 1/1000 dilution

Lane 1:

Mouse cerebellum lysate at 20 µg

Lane 2:

Mouse lung lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Lane 4:

Rat cerebellum lysate at 20 µg

Lane 5:

Rat lung lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 66 kDa

Observed band size: 67 kDa

true

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-GAD67/GAD1 antibody [EPR20578] (AB213508)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GAD67/GAD1 antibody [EPR20578] (ab213508) at 1/1000 dilution

All lanes:

Mouse brain lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 66 kDa

Observed band size: 67 kDa

true

Exposure time: 30s