Anti-GAD65 + GAD67抗体(ab49832)

Thank you for contacting us. We recommend that you use this antibody for IHC-P at a dilution of 1/1000, incuabted overnight. You should first block the tissue with 10% normal serum plus 1% BSA for 1-2 hours to diminish background.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the choice will be personal preference of host. These products are below. Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Ab16284: https://www.abcam.com/Rabbit-IgG-secondary-antibody-ab16284.html Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Ab97051: https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-HRP-ab97051.html Abcam also provides a number of IHC reagents and kits such as DAB substrate kits, donkey or goat sera and antigen retrieval buffers. All of our products are covered by our six month Abpromise guarantee and are fully supported by our scientific support staff. I have provided links to a few of these products below. DAB substrate kit:https://www.abcam.com/DAB-substrate-kit-ab94665.html Donkey serum:https://www.abcam.com/Donkey-Serum-ab7475.html Goat serum:https://www.abcam.com/Goat-Serum-ab7481.html Antigen retrieval buffer:https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Tris-EDTA-Buffer-pH-9-0-ab93684.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

Thank you for contacting us.

We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests.

All three of ourAnti-GAD65 + GAD67 antibodies are guaranteed to work in mouse tissues. Ab49832 and Ab11070 are both tested and guaranteed in IHC. I would be very happy to help you to find the best secondary antibody for use with either of these products. In order to determine this could you please let me know the detection system you plan to use?

I am sorry that I could not be more helpful regarding the best marker for your needs, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

Thank you for contacting us.
I am sorry to hearthat you are encountering some problems with this antibody.

As discussed over the phone, I am sending the link to our antigen retrieval protocols:

https://www.abcam.com/index.html?pageconfig=resource&rid=11488

When using IHC-P, then heat mediated antigen retreival in 10mM citrate buffer was performed prior to incubation with ab49832.

Also, blocking with more BSA, e.g. 5% , can help reducing the non-specific background.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

I am happy you received the antibody already, and hope it works as expected.
If not, please do let us know and we will be happy to assist you further.

Thank you for contacting us.


The anti-GAD65 + GAD67 antibody (ab49832) has to our knowledge not been used with free floating sections as yet and your customer would therefore be eligible for a testing discount. I have generated the code below:


Discount code: xxxxxxxxxx


Expiration date: 28th June2012


Once your customer has purchasedab49832, in order to claim their free primary antibody, they need to test the antibodywith staining of mousefree floatingsections and let us know of the results through submitting an Abreview. The discount code then needs to be put into the "additional comments" section to be activated. It can then be used to redeem one free primaryantibody fromour catalogue.


The discount code is valid for 4 months.


Ifyou require any further information please do not hesitate to contact us again.

As requested, I have issued a free of charge replacement with the order number XXXXXX from lot XXXXX of ab45429.

To check the status of the order please contact our Customer Service team and reference this number.

I hope this new vial works as expected.

Please do not hesitate to contact us if you need any more advice or information.