重组Anti-GABARAP抗体[EPR4806] (ab109299)
![Western blot - Anti-GABARAP antibody [EPR4806] (ab109299)](https://www.abcam.cn/ps/products/109/ab109299/Images/ab109299-122054-anti-gabarap-antibody-epr4806-western-blot.jpg "Western blot - Anti-GABARAP antibody [EPR4806] (ab109299)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4806] to GABARAP
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GABARAP抗体[EPR4806]
参阅全部 GABARAP 一抗 -
描述
兔单克隆抗体[EPR4806] to GABARAP -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- SH-SY5Y cell lysate, Human fetal brain tissue lysate, Flag-GABARAP, Human heart tissue, Human brain tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4806 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109299于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
|
| IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. -
组织特异性
Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. -
序列相似性
Belongs to the ATG8 family. -
翻译后修饰
The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II. -
细胞定位
Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity). - Information by UniProt
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数据库链接
- Entrez Gene: 11337 Human
- Entrez Gene: 58974 Rat
- Omim: 605125 Human
- SwissProt: O95166 Human
- SwissProt: P60517 Rat
- Unigene: 647421 Human
- Unigene: 8411 Rat
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别名
- ATG8A antibody
- FLC 3B antibody
- FLC3B antibody
see all
图片
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Western blot - Anti-GABARAP antibody [EPR4806] (ab109299)All lanes : Anti-GABARAP antibody [EPR4806] (ab109299) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate at 10 µg
Lane 2 : SH-SY5Y cell lysate at 10 µg
Lane 3 : Flag-GABARAP
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP antibody [EPR4806] (ab109299)](https://www.abcam.cn/ps/products/109/ab109299/Images/ab109299-122053-anti-gabarap-antibody-epr4806-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP antibody [EPR4806] (ab109299)ab109299, at 1/100 dilution, staining GABARAP in Human heart tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP antibody [EPR4806] (ab109299)](https://www.abcam.cn/ps/products/109/ab109299/Images/ab109299-122052-anti-gabarap-antibody-epr4806-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP antibody [EPR4806] (ab109299)ab109299, at 1/100 dilution, staining GABARAP in Human brain tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-GABARAP antibody [EPR4806] (ab109299)](https://www.abcam.cn/ps/products/109/ab109299/Images/ab109299-4-benefits-of-recombinant-antibodies.png)
Anti-GABARAP antibody [EPR4806] (ab109299)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab109299 被引用在 1 文献中.
- He C et al. Yunvjian-Medicated Serum Protects INS-1 Cells against Glucolipotoxicity-Induced Apoptosis through Autophagic Flux Modulation. Evid Based Complement Alternat Med 2020:8878259 (2020). PubMed: 33414841