AB109299

重组Anti-GABARAP抗体[EPR4806]

Name: GABARAP抗体[EPR4806] (ab109299)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109299
Price: 2558 CNY
Availability: InStock
Rating: 2 (3 reviews)

Anti-GABARAP antibody [EPR4806]

RabMAb
Recombinant
了解详情

(3 Reviews)

(3 Publications)

Rabbit Recombinant Monoclonal GABARAP antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.

查看别名

FLC3B, HT004, GABARAP, Gamma-aminobutyric acid receptor-associated protein, GABA(A) receptor-associated protein, MM46

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP antibody [EPR4806] (AB109299)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP antibody [EPR4806] (AB109299)

ab109299 at 1/100 dilution staining GABARAP in Human heart tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP antibody [EPR4806] (AB109299)

ab109299 at 1/100 dilution staining GABARAP in Human brain tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-GABARAP antibody [EPR4806] (AB109299)

All lanes:

Western blot - Anti-GABARAP antibody [EPR4806] (ab109299) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

Flag-GABARAP

Predicted band size: 13 kDa

Observed band size: 17 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-GABARAP antibody [EPR4806] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4806

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton (PubMed : 9892355). Involved in autophagy : while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed : 15169837, PubMed : 20562859, PubMed : 22948227). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed : 31006538). Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction (PubMed : 25684205). Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation (PubMed : 25684205). Involved in apoptosis (PubMed : 15977068).

See full target information GABARAP

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cell division 18:9 PubMed37301821

2023

SIRT4 is an independent prognostic factor in bladder cancer and inhibits bladder cancer growth by suppressing autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yin,Guohao Cai,Huaiwen Wang,Weijia Chen,Shan Liu,Guoyu Huang

Journal of Cancer 12:7422-7435 PubMed35003362

2021

Interaction of CD147 and human epididymis protein 4 promotes invasion and metastasis of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Gao,Xin Nie,Rui Gou,Yue Qi,Juanjuan Liu,Bei Lin

Evidence-based complementary and alternative medic 2020:8878259 PubMed33414841

2020

Yunvjian-Medicated Serum Protects INS-1 Cells against Glucolipotoxicity-Induced Apoptosis through Autophagic Flux Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Caigu He,Xuehua Zheng,Xiuhong Lin,Xinying Chen,Chenyi Shen

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-GABARAP抗体[EPR4806]