Anti-GABA B Receptor 2/GABBR2抗体(ab52248)
Key features and details
- Rabbit polyclonal to GABA B Receptor 2/GABBR2
- Suitable for: IHC-FoFr, WB
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GABA B Receptor 2/GABBR2抗体
参阅全部 GABA B Receptor 2/GABBR2 一抗 -
描述
兔多克隆抗体to GABA B Receptor 2/GABBR2 -
宿主
Rabbit -
经测试应用
适用于: IHC-FoFr, WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to Human GABA B Receptor 2/GABBR2.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52248于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FoFr | (1) |
Use at an assay dependent concentration.
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| WB |
1/300 - 1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
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| 说明 |
|---|
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IHC-FoFr
Use at an assay dependent concentration. |
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WB
1/300 - 1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa). |
靶标
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功能
Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. -
组织特异性
Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle. -
序列相似性
Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily. -
结构域
Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1. -
细胞定位
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9568 Human
- Entrez Gene: 83633 Rat
- Omim: 607340 Human
- SwissProt: O75899 Human
- SwissProt: O88871 Rat
- Unigene: 198612 Human
- Unigene: 162814 Rat
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别名
- BcDNA:GH07312 antibody
- CG6706 antibody
- CT20836 antibody
see all
图片
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Western blot - Anti-GABA B Receptor 2/GABBR2 antibody (ab52248)All lanes : Anti-GABA B Receptor 2/GABBR2 antibody (ab52248) at 1/300 dilution
Lane 1 : HeLa cell extract; without peptide
Lane 2 : HeLa cell extract; with peptide
Predicted band size: 106 kDa
Observed band size: 106 kDa -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-GABA B Receptor 2/GABBR2 antibody (ab52248)This image is courtesy of Karine Thibault, CNRS, Paris, FranceImmunohistochemistical detection of GABA B Receptor 2/GABBR2 antibody (ab52248) in PFA fixed Rat Dorsal root ganglion sections. ab52248 was used at 1/300 and incubated for 60 hours @ 4°C in PBS-T 0.3%. Secondary antibody: anti IgG Rabbit Conjugation: Alexa Fluor® 488 (1/1000).
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab52248 被引用在 8 文献中.
- Dasdelen MF et al. A Novel Theanine Complex, Mg-L-Theanine Improves Sleep Quality via Regulating Brain Electrochemical Activity. Front Nutr 9:874254 (2022). PubMed: 35449538
- Li K et al. Long Noncoding RNA H19 Induces Neuropathic Pain by Upregulating Cyclin-Dependent Kinase 5-Mediated Phosphorylation of cAMP Response Element Binding Protein. J Pain Res 13:2113-2124 (2020). PubMed: 32903807
- Tabassum S et al. Jujuboside A prevents sleep loss-induced disturbance of hippocampal neuronal excitability and memory impairment in young APP/PS1 mice. Sci Rep 9:4512 (2019). PubMed: 30872728
- Sarveazad A et al. Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury. Stem Cell Res Ther 10:183 (2019). PubMed: 31234929
- Fan J et al. Metformin produces anxiolytic-like effects in rats by facilitating GABAA receptor trafficking to membrane. Br J Pharmacol 176:297-316 (2019). PubMed: 30318707
- Li H et al. Low Cerebral Exposure Cannot Hinder the Neuroprotective Effects of Panax Notoginsenosides. Drug Metab Dispos 46:53-65 (2018). PubMed: 29061584
- Li Y et al. Validation and bioinformatic analysis of propofol-induced differentially expressed microRNAs in primary cultured neural stem cells. Gene 664:90-100 (2018). WB . PubMed: 29679758
- Thibault K et al. Molecular mechanisms underlying the enhanced analgesic effect of oxycodone compared to morphine in chemotherapy-induced neuropathic pain. PLoS One 9:e91297 (2014). WB, IHC-Fr, IHC-FrFl ; Rat . PubMed: 24618941