重组Anti-GABA B Receptor 1抗体[EPR22954-47]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling GABA B Receptor 1 with ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining on human liver. The section was incubated with ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238130).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130)

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling GABA B Receptor 1 with ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebellum. The section was incubated with ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238130).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling GABA B Receptor 1 with ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebellum. The section was incubated with ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238130).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling GABA B Receptor 1 with ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebellum. The section was incubated with ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238130).

• WB

Lab

Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All the lysates were unboiled.

Skeletal muscle is a low-expression tissue. T

he expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 26033241, 10996466).

Exposure time : Lane 1 3 minutes Lanes 2-3 : 50 seconds.

All lanes:

Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human cerebellum tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 108 kDa

Observed band size: 108 kDa,95 kDa

false

• WB

Lab

Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All the lysates were unboiled.

Skeletal muscle is a low-expression tissue.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 26033241, 10996466).

Exposure time : Lanes 1-2 : 75 seconds Lanes 3-4 : 3 minutes.

All lanes:

Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 108 kDa

Observed band size: 108 kDa,95 kDa

false