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重组RabMAb

重组Anti-GABA A Receptor alpha 1抗体[EPR5401(2)] (ab151573)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5401(2)] to GABA A Receptor alpha 1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-GABA A Receptor alpha 1抗体[EPR5401(2)]
    参阅全部 GABA A Receptor alpha 1 一抗

  • 描述

    兔单克隆抗体[EPR5401(2)] to GABA A Receptor alpha 1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human GABA A Receptor alpha 1 aa 1-100. The exact sequence is proprietary.

  • 阳性对照

    • WB: Human fetal brain, Human lung and Human cerebellum lysates; Human brain prepared in 1%SDS Hot lysis method. IHC-P: Human brain, colon, kidney, Ovarian carcinoma, spleen, cerebrum and glioma tissues.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab151573于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

We recommend to try 1% SDS Hot lysis prepare method to get desired WB results.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use 1/50 - 1/100

See IHC antigen retrieval protocols.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

We recommend to try 1% SDS Hot lysis prepare method to get desired WB results.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use 1/50 - 1/100

See IHC antigen retrieval protocols.

靶标

  • 功能

    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

  • 疾病相关

    Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
    Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.

  • 序列相似性

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.

  • 细胞定位

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ECA4 antibody
      • EIEE19 antibody
      • EJM antibody
      • EJM5 antibody
      • Gaba receptor alpha 1 polypeptide antibody
      • GABA(A) receptor antibody
      • GABA(A) receptor subunit alpha 1 antibody
      • GABA(A) receptor subunit alpha-1 antibody
      • GABA(A) receptor, alpha 1 antibody
      • GABRA 1 antibody
      • GABRA1 antibody
      • Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody
      • Gamma aminobutyric acid A receptor alpha 1 antibody
      • Gamma aminobutyric acid receptor subunit alpha 1 antibody
      • Gamma aminobutyric acid type A receptor alpha1 subunit antibody
      • Gamma-aminobutyric acid receptor subunit alpha-1 antibody
      • GBRA1_HUMAN antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling GABA A Receptor alpha 1 with Purified ab151573 at 1:1000 dilution (0.7 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
    • Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 1/1000 dilution

      Lane 1 : Human brain prepared in RIPA lysis method
      Lane 2 : Human brain prepared in 1%SDS Hot lysis method

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 52 kDa
      Observed band size: 52 kDa


      Exposure time: 40 seconds


      Blocking/Diluting buffer and concentration: 5% NFDM/TBST

      We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

    • Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 0.7 µg/ml (purified)

      Lane 1 : Human brain lysates
      Lane 2 : Human lung lysates - negative control

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 52 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.


      Please note: Lysates prepared in 1%SDS Hot lysis method.

      According to the paper (PMID: 29467616), brain is positive and lung is negative tissue.

    • Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human cerebellum lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 52 kDa



      Please note: Lysates prepared in 1%SDS Hot lysis method.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling GABA A Receptor alpha 1 with unpurified ab151573 at 1/50 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

      Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling GABA A Receptor alpha 1 with unpurified ab151573 at 1/50 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

      Immunohistochemical analysis of paraffin embedded normal Human colon tissue using unpurified ab151573 showing -ve staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

      Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using unpurified ab151573 showing -ve staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

      Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using unpurified ab151573 showing -ve staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

      Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using unpurified ab151573 showing -ve staining.

    • Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

    文献 (1)

    发表研究结果有使用 ab151573？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab151573 被引用在 1 文献中.

    • Tabassum S  et al. Jujuboside A prevents sleep loss-induced disturbance of hippocampal neuronal excitability and memory impairment in young APP/PS1 mice. Sci Rep 9:4512 (2019). PubMed: 30872728

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