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重组RabMAb

重组Anti-GAB2抗体[EP369Y] (ab32365)

Submit a review Q&A (2)References (4)
Western blot - Anti-GAB2 antibody [EP369Y] (ab32365)
  • Anti-GAB2 antibody [EP369Y] (ab32365)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP369Y] to GAB2
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-GAB2抗体[EP369Y]
    参阅全部 GAB2 一抗

  • 描述

    兔单克隆抗体[EP369Y] to GAB2

  • 宿主

    Rabbit

  • 特异性

    This antibody is predicted to detect the splice isoform 2 based on sequence homology.

  • 经测试应用

    适用于: WBmore details
    不适用于: ICC/IF,IHC or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human GAB2 aa 600-700. The exact sequence is proprietary.

  • 阳性对照

    • WB: K562 cell lysates

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab32365于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 74 kDa).
说明
WB
1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 74 kDa).
应用说明
Is unsuitable for ICC/IF,IHC or IP.

靶标

  • 功能

    Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.

  • 序列相似性

    Belongs to the GAB family.
    Contains 1 PH domain.

  • 结构域

    The SH3-binding motifs mediate interaction with SHC1 and GRB2.
    The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.

  • 翻译后修饰

    Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
    Dephosphorylated by PTPN11.

  • 细胞定位

    Cytoplasm. Cell membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • GAB 2 antibody
    • Gab2 antibody
    • GAB2_HUMAN antibody
    • Grb 2 associated binder 2 antibody
    • GRB 2 associated binding protein 2 antibody
    • Grb2 associated binder 2 antibody
    • GRB2 associated binder 2 pp100 antibody
    • GRB2 associated binding protein 2 antibody
    • GRB2-associated binder 2 antibody
    • GRB2-associated-binding protein 2 antibody
    • Growth factor receptor bound protein 2 associated protein 2 antibody
    • Growth factor receptor bound protein 2-associated protein 2 antibody
    • KIAA0571 antibody
    • p97 antibody
    • PH domain containing adaptor molecule p97 antibody
    • pp100 antibody
    see all

图片

  • Western blot - Anti-GAB2 antibody [EP369Y] (ab32365)
    Western blot - Anti-GAB2 antibody [EP369Y] (ab32365)
    All lanes : Anti-GAB2 antibody [EP369Y] (ab32365) at 1/500 dilution

    Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate, prepared in RIPA lysis method
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate, prepared in 1% SDS Hot lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 74 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?



    We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

    Blocking/Diluting Buffer and concentration:
    5% NFDM/TBST

  • Anti-GAB2 antibody [EP369Y] (ab32365)
    Anti-GAB2 antibody [EP369Y] (ab32365)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (4)

发表研究结果有使用 ab32365？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab32365 被引用在 4 文献中.

  • Sun J  et al. Anti-allergic actions of a Chinese patent medicine, huoxiangzhengqi oral liquid, in RBL-2H3 cells and in mice. Pharm Biol 59:672-682 (2021). PubMed: 34078224
  • Mu L  et al. MicroRNA-218 inhibits tumor angiogenesis of human renal cell carcinoma by targeting GAB2. Oncol Rep 44:1961-1970 (2020). PubMed: 32901879
  • Tian LQ  et al. MicroRNA-197 inhibits cell proliferation by targeting GAB2 in glioblastoma. Mol Med Rep N/A:N/A (2016). WB . PubMed: 27035789
  • Pyarajan S  et al. Interleukin-3 (IL-3)-induced c-fos Activation Is Modulated by Gab2-Calcineurin Interaction. J Biol Chem 283:23505-9 (2008). WB . PubMed: 18586679

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Dear Sirs,

I would like to query your technical support regarding the following antibodies:
Ab62802,ab32365, ab78262 and ab92578.
In order to detect changes in GAB2 and phospho-GAB2 levels we ordered several anti GAB2 and anti phospho-GAB2 antibodies from different sources.
After trying several cell lysis conditions to minimize breakdown products, we settled for the cell lysis buffer by Cell signaling technologies (cat #3884 in combination with a protease inhibitor cocktail from Roche). Result where generated using the K562 cell line which is also mentioned in the datasheet of Ab62802,ab32365 and ab92578
Using the competitors antibodies we are able to assure the availability of both GAB2 and phosphorylation site specific forms of phospho-GAB2.
Unfortunately using the anti GAB2 antibodies ab62802,ab32365,ab92578 and ab78262 we are not able to get the desired results.
Ab62802 does not yield a nice and clean band at the expected 80 to 90 KDa, instead we see a smear/aspecific binding to our membrane.
The antibodies ab32365,ab92578 and ab78262 do not yield a band when using either lysates of K562 or IFN triggered Jurkat cells (according to the ab78262 datasheet).
Since the competitors antibodies clearly show the availability of both GAB2 and pGAB2 in the K562 cell line we seriously doubt the effectiveness of the Ab62802,ab32365, ab78262 and ab92578 antibodies (please see attached pdf for a line up of the antibodies used (markings in blue are competitor products).
Our question is, do you have any suggestion to alleviate our problems, we are somewhat at a loss here.
Kind regards,

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Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In order to better understand the problem, I’d like to send you a questionnaire with some questions about the protocol used.

Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results.

According to the image sent the three anti phosphorilated GAB2 antibodies show different patterns, both #1 and #3 do not show any bands and #2 however gives several bands. Are the three antibodies targeting the same phosphorilated site in the protein?

I look forward to receiving your reply. Please do not hesitate to contact us if you need any more advice or information.

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Question


Inquiry: Do you cell a blocking peptide for GAB2 antibody?

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Answer

Hello! The blocking peptides for these antibodies have now been published in our catalog, they are ab133951 and ab133952. Please let me know if you have any further questions.

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