重组Anti-GAB1抗体[EPR375] (ab133486)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR375] to GAB1
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GAB1抗体[EPR375]
参阅全部 GAB1 一抗 -
描述
兔单克隆抗体[EPR375] to GAB1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human GAB1 aa 500-600. The exact sequence is proprietary.
Database link: Q13480 -
阳性对照
- WB: HepG2, K562, 293T, and SH-SY5Y cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR375 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133486于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
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IP |
1/10 - 1/100.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa. |
IP
1/10 - 1/100. |
靶标
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功能
Probably involved in EGF and insulin receptor signaling. -
序列相似性
Belongs to the GAB family.
Contains 1 PH domain. -
翻译后修饰
Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. - Information by UniProt
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数据库链接
- Entrez Gene: 2549 Human
- Omim: 604439 Human
- SwissProt: Q13480 Human
- Unigene: 618456 Human
- Unigene: 80720 Human
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别名
- Gab 1 antibody
- GAB1 antibody
- GAB1_HUMAN antibody
see all
图片
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: GAB1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Hek293 whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab133486 observed at 100 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab133486 was shown to recognize GAB1 in wild-type HAP1 cells as signal was lost at the expected MW in GAB1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GAB1 knockout samples were subjected to SDS-PAGE. Ab133486 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Anti-GAB1 antibody [EPR375] (ab133486) at 1/2000 dilution (purified) + K562 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 77 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
Anti-GAB1 antibody [EPR375] (ab133486) at 1/2000 dilution (purified) + HEK293 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 77 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
All lanes : Anti-GAB1 antibody [EPR375] (ab133486) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 77 kDa -
ab133486 (purified) at 1/80 immunoprecipitating GAB1 in HEK293 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/10,000) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab133486 被引用在 10 文献中.
- Nakata S et al. Epigenetic upregulation of Schlafen11 renders WNT- and SHH-activated medulloblastomas sensitive to cisplatin. Neuro Oncol 25:899-912 (2023). PubMed: 36273330
- Li J et al. Interference of PTK6/GAB1 signaling inhibits cell proliferation, invasion, and migration of cervical cancer cells. Mol Med Rep 26:N/A (2022). PubMed: 35894144
- Natsumeda M et al. GLI3 Is Associated With Neuronal Differentiation in SHH-Activated and WNT-Activated Medulloblastoma. J Neuropathol Exp Neurol 80:129-136 (2021). PubMed: 33249504
- Wu PF et al. Depletion of circRNA circ_CDK14 inhibits osteosarcoma progression by regulating the miR-520a-3p/GAB1 axis. Neoplasma 68:798-809 (2021). PubMed: 34348465
- He Q et al. Circ_0061012 contributes to IL-22-induced proliferation, migration and invasion in keratinocytes through miR-194-5p/GAB1 axis in psoriasis. Biosci Rep 41:N/A (2021). PubMed: 33393621
- Visani M et al. miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and Children. Diagnostics (Basel) 10:N/A (2020). PubMed: 32365560
- Carrie C et al. Exclusive Hyperfractionated Radiation Therapy and Reduced Boost Volume for Standard-Risk Medulloblastoma: Pooled Analysis of the 2 French Multicentric Studies MSFOP98 and MSFOP 2007 and Correlation With Molecular Subgroups. Int J Radiat Oncol Biol Phys 108:1204-1217 (2020). PubMed: 32768563
- Shuangshoti S et al. Simplified Molecular Subtyping of Medulloblastoma for Reduced Cost and Improved Turnaround Time. Appl Immunohistochem Mol Morphol 28:538-543 (2020). PubMed: 31343993
- Kaur K et al. Approach to molecular subgrouping of medulloblastomas: Comparison of NanoString nCounter assay versus combination of immunohistochemistry and fluorescence in-situ hybridization in resource constrained centres. J Neurooncol 143:393-403 (2019). PubMed: 31104222
- Kaur K et al. Integrating Molecular Subclassification of Medulloblastomas into Routine Clinical Practice: A Simplified Approach. Brain Pathol 26:334-43 (2016). PubMed: 26222673