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AB133486

Anti-GAB1 抗体 [EPR375]

Anti-GAB1 antibody [EPR375]

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(10 Publications)

Anti-GAB1 antibody [EPR375] (ab133486) is a rabbit monoclonal antibody detecting GAB1 in Western Blot, IP. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

查看别名

GRB2-associated-binding protein 1, GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1, GAB1

5 Images
Western blot - Anti-GAB1 antibody [EPR375] (AB133486)
  • WB

Unknown

Western blot - Anti-GAB1 antibody [EPR375] (AB133486)

All lanes:

Western blot - Anti-GAB1 antibody [EPR375] (ab133486) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 77 kDa

false

Immunoprecipitation - Anti-GAB1 antibody [EPR375] (AB133486)
  • IP

Unknown

Immunoprecipitation - Anti-GAB1 antibody [EPR375] (AB133486)

ab133486 (purified) at 1/80 immunoprecipitating GAB1 in HEK293 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/10,000) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-GAB1 antibody [EPR375] (ab133486)

Predicted band size: 77 kDa

false

Western blot - Anti-GAB1 antibody [EPR375] (AB133486)
  • WB

Lab

Western blot - Anti-GAB1 antibody [EPR375] (AB133486)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : GAB1 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : Hek293 whole cell lysate (20 μg)
Lane 4 : HepG2 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab133486 observed at 100 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab133486 was shown to recognize GAB1 in wild-type HAP1 cells as signal was lost at the expected MW in GAB1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GAB1 knockout samples were subjected to SDS-PAGE. ab133486 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GAB1 antibody [EPR375] (ab133486)

Predicted band size: 77 kDa

false

Western blot - Anti-GAB1 antibody [EPR375] (AB133486)
  • WB

Unknown

Western blot - Anti-GAB1 antibody [EPR375] (AB133486)

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-GAB1 antibody [EPR375] (ab133486) at 1/2000 dilution

All lanes:

K562 cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 77 kDa

false

Western blot - Anti-GAB1 antibody [EPR375] (AB133486)
  • WB

Unknown

Western blot - Anti-GAB1 antibody [EPR375] (AB133486)

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-GAB1 antibody [EPR375] (ab133486) at 1/2000 dilution

All lanes:

HEK293 cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 77 kDa

false

不同偶联物与剂型 (1)

  • Carrier free

    Anti-GAB1 antibody [EPR375] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR375

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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产品详情

What is this antibody validated in?
Anti-GAB1 antibody [EPR375] (ab133486) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human samples.

What is the molecular weight of GAB1?
Anti-GAB1 [EPR375] (ab133486) specifically detects a band for GAB1 (UniProt: Q13480) at a molecular weight of 77kDa.

Trusted by the scientific community
Anti-GAB1 [EPR375] (ab133486) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-GAB1 antibody [EPR375] (ab133486) has been confirmed by Western blot testing in GAB1 Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EPR375] also available for your convenience: ab133486, Carrier free - ab240062, Carrier free - ab281271

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway (PubMed : 29408807).
See full target information GAB1
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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Neuro-oncology 25:899-912 PubMed36273330

2022

Epigenetic upregulation of Schlafen11 renders 
WNT- and SHH-activated medulloblastomas sensitive to cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Nakata,Junko Murai,Masayasu Okada,Haruhiko Takahashi,Tyler H Findlay,Kristen Malebranche,Akhila Parthasarathy,Satoshi Miyashita,Ramil Gabdulkhaev,Ilan Benkimoun,Sabine Druillennec,Sara Chabi,Eleanor Hawkins,Hiroaki Miyahara,Kensuke Tateishi,Shinji Yamashita,Shiori Yamada,Taiki Saito,Jotaro On,Jun Watanabe,Yoshihiro Tsukamoto,Junichi Yoshimura,Makoto Oishi,Toshimichi Nakano,Masaru Imamura,Chihaya Imai,Tetsuya Yamamoto,Hideo Takeshima,Atsuo T Sasaki,Fausto J Rodriguez,Sumihito Nobusawa,Pascale Varlet,Celio Pouponnot,Satoru Osuka,Yves Pommier,Akiyoshi Kakita,Yukihiko Fujii,Eric H Raabe,Charles G Eberhart,Manabu Natsumeda

Molecular medicine reports 26: PubMed35894144

2022

Interference of PTK6/GAB1 signaling inhibits cell proliferation, invasion, and migration of cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Li,Nan Yang,Xiaolei Tian,Linglong Ouyang,Man Jiang,Shufang Zhang

Neoplasma 68:798-809 PubMed34348465

2021

Depletion of circRNA circ_CDK14 inhibits osteosarcoma progression by regulating the miR-520a-3p/GAB1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Peng-Fei Wu,Xiao-Ming Tang,Hai-Lang Sun,Hai-Tao Jiang,Jian Ma,Ying-Hui Kong

Bioscience reports 41: PubMed33393621

2021

Circ_0061012 contributes to IL-22-induced proliferation, migration and invasion in keratinocytes through miR-194-5p/GAB1 axis in psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Qi He,Nian Liu,Feng Hu,Quan Shi,Xianming Pi,Hongxiang Chen,Jiawen Li,Bo Zhang

Journal of neuropathology and experimental neurology 80:129-136 PubMed33249504

2020

GLI3 Is Associated With Neuronal Differentiation in SHH-Activated and WNT-Activated Medulloblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Manabu Natsumeda,Hiroaki Miyahara,Junichi Yoshimura,Satoshi Nakata,Takanori Nozawa,Junko Ito,Yu Kanemaru,Jun Watanabe,Yoshihiro Tsukamoto,Masayasu Okada,Makoto Oishi,Junko Hirato,Takafumi Wataya,Sama Ahsan,Kensuke Tateishi,Tetsuya Yamamoto,Fausto J Rodriguez,Hitoshi Takahashi,Volker Hovestadt,Mario L Suva,Michael D Taylor,Charles G Eberhart,Yukihiko Fujii,Akiyoshi Kakita

International journal of radiation oncology, biolo :1204-1217 PubMed32768563

2020

Exclusive Hyperfractionated Radiation Therapy and Reduced Boost Volume for Standard-Risk Medulloblastoma: Pooled Analysis of the 2 French Multicentric Studies MSFOP98 and MSFOP 2007 and Correlation With Molecular Subgroups.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Carrie,Virginie Kieffer,Dominique Figarella-Branger,Julien Masliah-Planchon,Stéphanie Bolle,Valérie Bernier,Anne Laprie,Stéphane Supiot,Julie Leseur,Jean-Louis Habrand,Claire Alapetite,Christine Kerr,Christelle Dufour,Line Claude,Sophie Chapet,Aymeri Huchet,Pierre-Yves Bondiau,Alexandre Escande,Gilles Truc,Tan Dat Nguyen,Caroline Pasteuris,Céline Vigneron,Xavier Muracciole,Franck Bourdeaut,Romain Appay,Bernard Dubray,Carole Colin,Céline Ferlay,Sophie Dussart,Sylvie Chabaud,Laetitia Padovani

Diagnostics (Basel, Switzerland) 10: PubMed32365560

2020

miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and Children.

Applications

Unspecified application

Species

Unspecified reactive species

Michela Visani,Gianluca Marucci,Dario de Biase,Felice Giangaspero,Francesca Romana Buttarelli,Alba Ariela Brandes,Enrico Franceschi,Giorgia Acquaviva,Alessia Ciarrocchi,Kerry Jane Rhoden,Giovanni Tallini,Annalisa Pession

Applied immunohistochemistry & molecular morphology : AIMM 28:538-543 PubMed31343993

2019

Simplified Molecular Subtyping of Medulloblastoma for Reduced Cost and Improved Turnaround Time.

Applications

Unspecified application

Species

Unspecified reactive species

Somruetai Shuangshoti,Paveen Tadadontip,Piti Techavichit,Paul S Thorner,Shanop Shuangshoti,Chinachote Teerapakpinyo

Journal of neuro-oncology 143:393-403 PubMed31104222

2019

Approach to molecular subgrouping of medulloblastomas: Comparison of NanoString nCounter assay versus combination of immunohistochemistry and fluorescence in-situ hybridization in resource constrained centres.

Applications

Unspecified application

Species

Unspecified reactive species

Kavneet Kaur,Prerana Jha,Pankaj Pathak,Vaishali Suri,Mehar Chand Sharma,Ajay Garg,Ashish Suri,Chitra Sarkar

Brain pathology (Zurich, Switzerland) 26:334-43 PubMed26222673

2015

Integrating Molecular Subclassification of Medulloblastomas into Routine Clinical Practice: A Simplified Approach.

Applications

Unspecified application

Species

Unspecified reactive species

Kavneet Kaur,Aanchal Kakkar,Anupam Kumar,Supriya Mallick,Pramod K Julka,Deepak Gupta,Ashish Suri,Vaishali Suri,Mehar C Sharma,Chitra Sarkar
View all publications
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详情请参阅我们的条款与条件。
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