Anti-GAB1抗体(ab59362)
Key features and details
- Rabbit polyclonal to GAB1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-GAB1抗体
参阅全部 GAB1 一抗 -
描述
兔多克隆抗体to GAB1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
-
免疫原
Synthesized non phosphopeptide derived from human GAB1 around the phosphorylation site of tyrosine 659 (VDYPVV).
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit antiserum by affinity chromatography, using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59362于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
|
|
| WB |
1/500 - 1/1000. Detects a band of approximately 77 kDa.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
|
WB
1/500 - 1/1000. Detects a band of approximately 77 kDa. |
|
IHC-P
Use at an assay dependent concentration. |
靶标
-
功能
Probably involved in EGF and insulin receptor signaling. -
序列相似性
Belongs to the GAB family.
Contains 1 PH domain. -
翻译后修饰
Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. - Information by UniProt
-
数据库链接
- Entrez Gene: 2549 Human
- Entrez Gene: 14388 Mouse
- Entrez Gene: 361388 Rat
- Omim: 604439 Human
- SwissProt: Q13480 Human
- SwissProt: Q9QYY0 Mouse
- Unigene: 618456 Human
- Unigene: 80720 Human
see all -
别名
- Gab 1 antibody
- GAB1 antibody
- GAB1_HUMAN antibody
see all
图片
-
Western blot - Anti-GAB1 antibody (ab59362)All lanes : Anti-GAB1 antibody (ab59362) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3 cells with no immunizing peptide
Lane 2 : Extracts from NIH/3T3 cells with immunizing peptide
Observed band size: 77 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAB1 antibody (ab59362)ab59362, at a 1/50 dilution, staining human GAB1 in breast carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image). -
Immunocytochemistry/ Immunofluorescence - Anti-GAB1 antibody (ab59362)ICC/IF image of ab59362 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59362, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (6)
ab59362 被引用在 6 文献中.
- Qiu N et al. LncRNA TUG1 alleviates sepsis-induced acute lung injury by targeting miR-34b-5p/GAB1. BMC Pulm Med 20:49 (2020). PubMed: 32087725
- Liu H et al. MiR-194-5p inhibited metastasis and EMT of nephroblastoma cells through targeting Crk. Kaohsiung J Med Sci 36:265-273 (2020). PubMed: 31889432
- Zhao T et al. DNA methylation-regulated QPCT promotes sunitinib resistance by increasing HRAS stability in renal cell carcinoma. Theranostics 9:6175-6190 (2019). PubMed: 31534544
- Hu Y et al. Inhibition of microRNA-29a alleviates hyperoxia-induced bronchopulmonary dysplasia in neonatal mice via upregulation of GAB1. Mol Med 26:3 (2019). PubMed: 31892308
- Hu L & Liu R Expression of Gab1 Is Associated with Poor Prognosis of Patients with Epithelial Ovarian Cancer. Tohoku J Exp Med 239:177-84 (2016). PubMed: 27302321
- Zhang Y et al. Identification of GRB2 and GAB1 coexpression as an unfavorable prognostic factor for hepatocellular carcinoma by a combination of expression profile and network analysis. PLoS One 8:e85170 (2013). IHC . PubMed: 24391994